The influence of manure-based organic fertilisers on the oviposition behaviour of Anopheles arabiensis.

The rice agroecosystem provides suitable breeding habitat for many malaria vector species, and  rice-adjacent communities are consequently exposed to a greater malaria transmission risk than non-rice-associated communities. As part of efforts to expand rice production in Africa, sustainable and climate-adapted practices such as the System of Rice Intensification (SRI) are being promoted. SRI encourages the use of organic fertilisers (OFs) such as cow and chicken dung, as opposed to inorganic industrially produced fertilisers, due to their lower resource cost, apparent benefit to the rice agroecosystem and as a means to reduce the greenhouse gas emissions associated with the production of industrial fertilisers. However, the impact of OFs on mosquito fauna is not well documented and may have knock-on consequences on malaria transmission risk. Here, we demonstrate, using dual choice egg count assays, that both cow and chicken dung modulate the oviposition behaviour of Anopheles arabiensis, a major malaria vector in Sub-Saharan Africa. A significantly reduced proportion of eggs were laid in water treated with either cow or chicken dung compared to untreated water, with higher dung concentrations resulting in further reduced proportions. When presented in competition, significantly fewer eggs were laid in water treated with chicken dung than with cow dung. Moreover, there was no evidence of egg retention in any experiment, including in no-choice experiments where only dung-containing dishes were available. These results suggest both cow and chicken dung may act as oviposition deterrents to malaria vector species and that the application of manure-based OFs in rice agriculture may modulate the oviposition behaviour of An. gambiae s.l. within agroecosystems. Quantification of the ammonia present in dung-infused water showed higher concentrations were present in the chicken dung infusion, which may be one contributing factor to the difference in observed deterrence between the two dung types. Deterrence of mosquito oviposition in OF-treated farms may potentially affect the overall production of malaria vectors within rice fields and their contribution to local malaria transmission.

Manure and mosquitoes: life history traits of two malaria vector species enhanced by larval exposure to cow dung, whilst chicken dung has a strong negative effect.

BACKGROUND: Malaria vectors have a strong ecological association with rice agroecosystems, which can provide abundant aquatic habitats for larval development. Climate-adapted rice cultivation practices, such as the System of Rice Intensification (SRI), are gaining popularity in malaria-endemic countries seeking to expand rice production; however, the potential impact of these practices on vector populations has not been well characterised. In particular, SRI encourages the use of organic fertilisers (OFs), such as animal manures, as low-cost and environmentally friendly alternatives to industrially produced inorganic fertilisers. We therefore set out to understand the effects of two common manure-based OFs on the life history traits of two major African malaria vectors, Anopheles arabiensis and Anopheles gambiae sensu stricto (s.s.). METHODS: Larvae of An. arabiensis and An. gambiae s.s. were reared from first instar to emergence in water containing either cow or chicken dung at one of four concentrations (0.25, 0.5, 0.75, and 1.0 g/100 ml), or in a clean water control. Their life history traits were recorded, including survival, development rate, adult production, and adult wing length. RESULTS: Exposure to cow dung significantly increased the development rate of An. gambiae s.s. independent of concentration, but did not affect the overall survival and adult production of either species. Chicken dung, however, significantly reduced survival and adult production in both species, with a greater effect as concentration increased. Interestingly, An. arabiensis exhibited a relative tolerance to the lowest chicken dung concentration, in that survival was unaffected and adult production was not reduced to the same extent as in An. gambiae s.s. The effects of chicken dung on development rate were less clear in both species owing to high larval mortality overall, though there was some indication that it may reduce development rate. Adult wing lengths in males and females increased with higher concentrations of both cow and chicken dung. CONCLUSIONS: Our findings suggest that manure-based OFs significantly alter the life history traits of An. gambiae s.s. and An. arabiensis. In both species, exposure to cow dung may improve fitness, whereas exposure to chicken dung may reduce it. These findings have implications for understanding vector population dynamics in rice agroecosystems and may inform the use of OFs in SRI, and rice agriculture more widely, to avoid their adverse effects in enhancing vector fitness.