RESUMO
23S ribosomal RNa (rRNA) gene sequences of Streptococcus uberis and Strep. parauberis were determined by direct polymerase chain reaction (PCR) sequencing. Oligonucleotide probes specific for Strep. uberis and Strep. parauberis were designed from variable regions of the 23S rRNA gene sequence data. Molecular hybridizations with PCR-amplified rRNA gene targets provided a precise and reliable means of differentiating Strep. uberis and Strep. parauberis from each other and from other streptococcal species.
Assuntos
DNA Ribossômico/genética , Sondas de Oligonucleotídeos , RNA Bacteriano/genética , RNA Ribossômico 23S/genética , Streptococcus/classificação , Técnicas de Tipagem Bacteriana , Sequência de Bases , DNA Bacteriano/genética , Genes Bacterianos , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Reação em Cadeia da Polimerase , Streptococcus/genéticaRESUMO
The large-subunit ribosomal RNA gene sequences of Leuconostoc carnosum, Leuc. mesenteroides, Leuc. oenos, Leuc. paramesenteroides and Lactobacillus confusus were determined by direct sequencing of enzymatically-amplified DNA. Comparative analysis of the large-subunit rDNA sequences revealed that the leuconostocs form three distinct lines of descent: Leuconostoc sensu stricto, Leuc. paramesenteroides group of organisms, and Leuc. oenos. The distance matrix tree constructed from the large-subunit sequence data was in excellent agreement with that derived from small-subunit sequences, and demonstrates the 'synchrony' of the two chronometers. The results of the present and earlier sequence analyses indicate the taxonomy of the genus Leuconostoc is in need of revision, and that the Leuc. paramesenteroides group of organisms and the species Leuc. oenos represent distinct phylogenetic units, separate from Leuconostoc sensu stricto.
Assuntos
DNA Ribossômico/genética , Leuconostoc/classificação , Filogenia , RNA Ribossômico 23S/genética , Sequência de Bases , DNA Bacteriano/genética , Genes Bacterianos , Leuconostoc/genética , Dados de Sequência Molecular , RNA Bacteriano/genética , Homologia de Sequência do Ácido NucleicoRESUMO
Genes encoding 16S rRNA were sequenced from 16 species of Staphylococcus. Sequence analysis highlighted a potential Staph. aureus-specific region and a complementary oligonucleotide probe was synthesized and its specificity tested. Northern blotting indicated molecular specificity, and dot blots to RNA from Staph. aureus, Staph. capitis, Staph. caprae, Staph. carnosus, Staph. caseolyticus, Staph. cohnii, Staph. epidermidis, Staph. gallinarum, Staph. haemolyticus, Staph. hominis, Staph. hyicus, Staph. saprophyticus, Staph. sciuri, Staph. simulans, Staph. warneri and Staph. xylosus indicated species-specificity.
