Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes.

AIM: The main purpose of the present study was to test the ß-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus. MATERIALS AND METHODS: Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out based on ßtub and rodA partial gene sequences. RESULTS: A 198 bp DNA fragment was obtained using ßtub gene. PCR amplification of the rodA gene resulted in a 313 bp band. The ßtub and rodA genes PCR products exhibited a 100% homology with the associated sequences in the GenBank. CONCLUSION: In the present study, we used a PCR approach that was able to discriminate A. fumigatus from other related species within the section Fumigati.