Reproducibilidad de la prueba de sobrecarga hídrica / Reproducibility of the water drinking test

OBJETIVOS: Investigar la reproducibilidad de la prueba de sobrecarga hídrica para determinar picos y fluctuación de presión intraocular. Se ha sugerido que la concordancia de la prueba de sobrecarga hídrica con la curva horaria es baja, sin embargo, solo se ha comparado con la curva horaria modificada de 10 horas, prescindiendo del 70% de los picos de presión ocular al no obtener las tomas nocturnas de presión intraocular. MÉTODOS: Estudio prospectivo, analítico y comparativo, en el que se determinó estadísticamente la correlación, la concordancia, así como la sensibilidad y la especificidad de la prueba de sobrecarga hídrica comparándola con la curva horaria diurna. RESULTADOS: La correlación de la prueba de sobrecarga hídrica fue alta (r = 0,93, IC 95% 0,79-0,96, p < 0,01), mientras que la concordancia de la prueba fue moderada (pc = 0,93, IC 95% 0,87-0,95, p < 0,01). La concordancia de valores entre +- 2 mm Hg (variabilidad intraobservador) fue del 89,5% IC 95%, 88-99%. DISCUSIÓN: En nuestro estudio se encontró una concordancia moderada contrastando con la pobre concordancia encontrada en otros estudios entre la prueba de sobrecarga hídrica y la curva horaria, posiblemente generada por la ausencia de tomas nocturnas de presión intraocular. CONCLUSIÓN: Estos hallazgos nos sugieren que la sobrecarga hídrica se puede utilizar para determinar picos de presión intraocular en quien se sospecha así como para determinar presión intraocular basal dada la concordancia encontrada en la variabilidad intraobservador

OBJECTIVES: To investigate the reproducibility of the water drinking test in determining intraocular pressure peaks and fluctuation. It has been suggested that there is limited agreement between the water drinking test and diurnal tension curve. This may be because it has only been compared with a 10-hour modified diurnal tension curve, missing 70% of IOP peaks that occurred during night. METHODS: This was a prospective, analytical and comparative study that assesses the correlation, agreement, sensitivity and specificity of the water drinking test. RESULTS: The correlation between the water drinking test and diurnal tension curve was significant and strong (r = 0.93, Confidence interval 95% between 0.79 and 0.96, p < 01). A moderate agreement was observed between these measurements (pc = 0.93, Confidence interval 95% between 0.87 and 0.95, p < .01). The agreement was within ± 2 mmHg in 89% of the tests. DISCUSSION: Our study found a moderate agreement between the water drinking test and diurnal tension curve, in contrast with the poor agreement found in other studies, possibly due to the absence of nocturnal IOP peaks. CONCLUSIONS: These findings suggest that the water drinking test could be used to determine IOP peaks, as well as for determining baseline IOP

Reproducibility of the water drinking test.

OBJECTIVES: To investigate the reproducibility of the water drinking test in determining intraocular pressure peaks and fluctuation. It has been suggested that there is limited agreement between the water drinking test and diurnal tension curve. This may be because it has only been compared with a 10-hour modified diurnal tension curve, missing 70% of IOP peaks that occurred during night. METHODS: This was a prospective, analytical and comparative study that assesses the correlation, agreement, sensitivity and specificity of the water drinking test. RESULTS: The correlation between the water drinking test and diurnal tension curve was significant and strong (r=0.93, Confidence interval 95% between 0.79 and 0.96, p<01). A moderate agreement was observed between these measurements (pc=0.93, Confidence interval 95% between 0.87 and 0.95, p<.01). The agreement was within±2mmHg in 89% of the tests. DISCUSSION: Our study found a moderate agreement between the water drinking test and diurnal tension curve, in contrast with the poor agreement found in other studies, possibly due to the absence of nocturnal IOP peaks. CONCLUSIONS: These findings suggest that the water drinking test could be used to determine IOP peaks, as well as for determining baseline IOP.

Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm.

Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.