Occurrence of fungal microbial contamination in drinking water of megacity of Karachi (Pakistan) and their physico-chemical control.

The water quality in Karachi (Pakistan) is uncertain due to the occurrence of fungi and other microorganisms. A total of twenty-five water samples were collected from public places, educational institutes, hospitals, water supply systems and surface water of the canal of Karachi (Pakistan). The different fungal species including Acremonium sp., Alternaria alternata, Aspergillus flavus, A. fumigatus, A. sulphureus, Cladosporium sp., Fusarium sp., Clonostachys (Gliocladium) sp., Macrophomina phaseolina, Mucor racemosus, Paecilomyces sp. Penicillium chrysogenum, P. citrinum, P. commune, P. expansum, Rhizoctonia sp. and Stachybotrys sp. were isolated from these drinking water samples. However, the bacteria, microalgae and some other microorganisms were present in low concentrations. The reason for fungi infection and production of mycotoxicity depends upon various factors and the availability of their nutrients in filtration plants. The major threats to human health are fungal mycotoxicity which is responsible for carcinogenic and other lethal diseases. Mostly, the genus Aspergillus was dominated and isolated with a maximum of 88-98% of occurrence in the different samples of drinking water by the direct plate-spread method. For the control of fungi, various Physico-chemical coagulation treatments were used, but Potassium alum, clay pot, and hot water treatment disinfected effectively 69-70% removal of the fungi and its spore or mycelia from the water. In addition, it is concluded that drinking water purifications such as chlorination, filtration and lime did not eliminate thermophilic fungal spores or mycelia including Penicillium, Paecilomyces and Mucor from the water.

Health risk assessment of heavy metals in exposed workers of municipal waste recycling facility in Iran.

Exposure to heavy metals (HMs) present in the particulate matter from municipal solid waste during pretreatment and recycling processes may pose a serious health risk to workers. This was the first study on the exposure of municipal solid waste (MSW) recycling workers to toxic metals. The concentrations of HMs (Cd, Pb, As, Co, Cr, Ni, Cu, Fe, Mn, and Zn) during personal exposure to PM2.5 among municipal waste recycling facility workers in Kashan City, Iran, were investigated from January 15 to March 15, 2023. The research was performed in the three main stages of the waste recycling process: dismantling, sorting, and collecting. PM2.5 samples were collected using a personal environmental monitor (PEM) attached to a sampling pump. The non-carcinogenic and carcinogenic health risk values and related uncertainty for waste recyclers from HMs inhalational exposure were calculated using USEPA methodology and Monte Carlo simulations. The results showed that the dismantlers exhibited the highest exposure concentrations of PM2.5 (mean 2148 ± 1257 µg m-3), followed by sorters (mean 1864 ± 965 µg m-3), and collectors (mean 1782 ± 876 µg m-3). Health risk assessment indicated that 95th percentile contents of Ni, As, Co, and Zn were responsible for the non-carcinogenic risk (HQ) values exceeding the acceptable level of 1. The contents of As, Ni, and Cr in PM2.5 caused a non-acceptable carcinogenic risk for waste recyclers due to inhalational exposure, as the carcinogenic risk (CR) values exceeded the acceptable threshold of 1 × 10-6. Monte Carlo simulation results revealed that the mean and median CR values from inhalational exposure to carcinogenic HMs exceeded the acceptable level of 1 × 10-6 for municipal waste recyclers. As results of this study indicated the high-risk to hazardous metals for waste recyclers due to occupational exposure in the MSW recycling sites, it is suggested to consider workers' exposure as the public health concern.

Pesticide Safety Awareness among Rural Farmers in Dadinkowa, Gombe State, Nigeria.

INTRODUCTION: Because of the longer growing season and warmer climate, weeds and insect pests spread are on the rise, thereby increasing the demand for pesticide use and consequently harmful emissions that further exacerbate climate change. Unsafe occupational exposure to pesticide residue is associated with a lack of product knowledge and safety awareness among farmers in low- and middle-income countries (LMICs). MATERIALS AND METHODS: A cross-sectional descriptive study design was adopted for this study in which a face-to-face administered questionnaire was used to collect data from 285 respondents who were selected using convenient snowball sampling technique. Knowledge, awareness, and practices related to pesticide storage, handling, application, and containers disposal among the farmers were measured. Categorical variables were analysed and presented using descriptive statistics in the form of frequency count and percentage, while numeric items were summarized using mean and standard deviation. RESULTS: Dichlorvos and Perfekthion 2.5 EC listed in the WHO Group I pesticide classification were among the most frequently used pesticides. Symptoms of pesticide intoxication reported include headaches (56.1%), dizziness (56.5%), skin irritation (53.3%), and fatigue (45.6%), respectively. Farmers' behaviour during pesticide application include blowing clogged nozzle with mouth (42.7%), talking while spraying (59.8%), and mixing pesticide with bare hands (31.1%). Furthermore, 38.5% of them use pesticide containers for other domestic purposes. CONCLUSIONS: Socioeconomic factors, i.e., educational level, age, and years of farm practice, influenced farmers safety behaviour. Based on these findings, an approach that will help strengthen capacity building programmes and the enhancement of knowledge-based initiatives around the adoption of non-synthetic pest-control methods should be encouraged.

Indoor Air Quality Monitoring and Characterization of Airborne Workstations Pollutants within Detergent Production Plant.

The indoor air quality (IAQ) of five workstations within a detergent production unit was monitored. Particulate matter (PM) was measured using a gravitational settlement method, and later characterized. To ascertain the quality of indoor air within the workstations, which could directly or indirectly affect the health and performance of the workers, a physical inspection of the plant premises was undertaken. The mean value of the following air-quality parameters; particulate matter(PM2.5), particulate matter (PM10), formaldehyde (HCHO), volatile organic compounds (VOCs), carbon dioxide (CO2), temperature (T) and percent relative humidity (%RH) were obtained within the range of 24.5-48.5 µg/m3, 26.75-61.75 µg/m3, 0.0-0.012 mg/m3, 0.09-1.35 mg/m3, 1137-1265 ppm, 25.65-28.15 °C and 20.13-23.8%, respectively. Of the particulate matter components characterized, sodium oxide (Na2O)-25.30 mg/m3, aluminum oxide (Al2O3)-22.93 mg/m3, silicon dioxide (SiO2)-34.17 mg/m3, sulfur trioxide (SO3)-41.57 mg/m3, calcium oxide (CaO)-10.94 mg/m3 and iron III oxide (Fe2O3)-19.23 mg/m3, were of significance. These results, compared with international standards for industrial indoor air quality, suggest that indoor air contamination emanating from the chemicals used in production workstations is traced to the design of the plant structures and the activities carried out within the workstations.

Interventions to Reduce Pesticide Exposure from the Agricultural Sector in Africa: A Workshop Report.

Despite the fact that several cases of unsafe pesticide use among farmers in different parts of Africa have been documented, there is limited evidence regarding which specific interventions are effective in reducing pesticide exposure and associated risks to human health and ecology. The overall goal of the African Pesticide Intervention Project (APsent) study is to better understand ongoing research and public health activities related to interventions in Africa through the implementation of suitable target-specific situations or use contexts. A systematic review of the scientific literature on pesticide intervention studies with a focus on Africa was conducted. This was followed by a qualitative survey among stakeholders involved in pesticide research or management in the African region to learn about barriers to and promoters of successful interventions. The project was concluded with an international workshop in November 2021, where a broad range of topics relevant to occupational and environmental health risks were discussed such as acute poisoning, street pesticides, switching to alternatives, or disposal of empty pesticide containers. Key areas of improvement identified were training on pesticide usage techniques, research on the effectiveness of interventions targeted at exposure reduction and/or behavioral changes, awareness raising, implementation of adequate policies, and enforcement of regulations and processes.

Cross-sectional survey of knowledge and attitudes of healthcare workers and community members toward the Ebola virus disease and antimicrobial resistance pathogens outbreaks in Nigeria.

INTRODUCTION: the 2014 Ebola virus disease (EVD) outbreak in Nigeria has further raised the awareness of health-care workers (HCWs) and community members (MCs) on the threat posed by infectious diseases and the need for improvement on infection control practices. However, awareness of dangers of increasing incidences of antimicrobial resistance (AMR) in hospitals and communities remained low. METHODS: a cross-sectional survey of awareness of 195 HCWs and 265 MCs toward EVD and AMR was conducted through a structured questionnaire. RESULTS: majority of HCWs (95.4%) and MCs (82.8%) still have knowledge of EVD´s danger and give reasons like its unique way of killing and unavailability of drugs for their awareness. Only 17.2% of MCs are aware of AMR as a problem, and only 3.4% of MCs and 10.3% of HCWs agreed that AMR is more dangerous than EVD. On the contrary, 76.4% of doctors, 95.1% nurses, 67.9% laboratory scientists, 66.7% pharmacists, 77.4% students and 100% of civil servants, drivers and religious leaders believed that EVD is more horrific and spread faster. They both attributed the rapid awareness of EVD in Nigeria, despite being new at the time of the outbreak, to the seriousness with which stakeholders and the media fought EVD, the gesture AMR is yet to receive. Though both HCWs and MCs agreed that prevention, not treatment is the best option to tackle Ebola like-diseases, but surprisingly, about 37% and 65% of HCWs and MCs respectively, still believe that traditional medicines can be used to treat Ebola related illnesses. CONCLUSION: AMR awareness remains low among MCs and some HCWs when compared with EVD. It is recommended that efforts put in place during EVD outbreak by all stakeholders and the media need to be doubled to increase the knowledge of both HCWs and MCs toward AMR.

Assessment of Workplace Safety Climate among Healthcare Workers during the COVID-19 Pandemic in Low and Middle Income Countries: A Case Study of Nigeria.

The COVID-19 pandemic has presented several organizations with the opportunity to review their operational strategies, as well as the existing safety climate within their establishments. The healthcare sector is not an exception, especially those in Low and Middle Income Countries (LMICs), where most safety systems are not robust when compared with developed countries. The study aim is to assess the occupational safety climate among healthcare workers (HCWs) in LMICs using Nigeria as a case study. A cross-sectional study was adopted to measure safety climate perception among professionals working in healthcare establishment during the COVID-19 pandemic using a validated Nordic Safety Climate Questionnaire (NOSACQ-50). At the end of the survey period, 83% (433) of the responses were adjudged to have met the threshold criteria and were used to inform the study outcome. Worker safety commitment within the healthcare facilities (M = 3.01, SD = 0.42) was statistically significantly higher than management safety priority, commitment, and competence (M = 2.91, SD = 0.46), t(130.52), p < 0.001. A significant effect of the management role was found in regards to management safety priority, commitment, and competence (F(1, 406) = 3.99, p = 0.046, η2 = 0.010). On the contrary, the managerial position does not have a significant effect on worker safety commitment (F(1, 417) = 0.59, p = 0.440, η2 = 0.001). The outcome from the study showed that, where there is active promotion of a positive safety climate in healthcare sectors in LMICs, employees are more likely to engage in positive safety behaviour. To help address the identified gaps, there is the need for more effort to be made towards promoting an effective and positive safety climate across the establishment, including management and healthcare worker commitments.

Assessment of Indoor Air Quality and Users Perception of a Renovated Office Building in Manchester.

Building renovations can adversely affect building occupants through the release of biological contaminants, gases and particulates. In this study, the research aim was to monitor the air quality of a renovated building and assess the impact of sick building syndrome (SBS) on the occupants. Post occupancy monitoring of the building was carried out after two months occupancy for the following environmental parameters: airborne microflora using an air sampler (SAS super 180) and a hand-held monitoring device (Graywolf advance sense IQ-610) to measure total volatile organic compounds (TVOC), CO2, CO and temperature and relative humidity in each office environment. In addition, an online (Qualtrics) structured questionnaire was used to assess occupants' perceptions of the indoor environment. Results of the airborne flora showed 833 cfu/m3 recovered on a Malt Extract Agar (MEA) plate in the morning and 1213 cfu/m3 in the afternoon. A similar result was noticed on a Plate Count Agar (PCA) plate during the morning period (731 cfu/m3) and afternoon (1358 cfu/m3). Results of TVOC monitored over one week showed that the first two days of monitoring had a high reading that peaked at 10,837 ppb and that the CO2 concentration during that period was 1163 ppm. Online questionnaire analysis indicates that a majority of the staff who took part in the survey experienced some form of health abnormality, including headache, shortness of breath, itchy eyes/ears, loss of concentration and so on, especially in the first few weeks of returning to the office. The results from the study indicate that a large proportion (41%) of the respondents experienced thermal discomfort as a result of varying room temperature during their working hours. A high number of female participants experienced some form of SBS as compared to their male counterparts. The study findings show a direct relationship between high airborne mold counts, TVOC and adverse staff health perception of the building. The study raised a number of opportunities for estate managers to improve building performance based on occupants' preferences.

Selective biodegradation of recalcitrant black chicken feathers by a newly isolated thermotolerant bacterium Pseudochrobactrum sp. IY-BUK1 for enhanced production of keratinase and protein-rich hydrolysates.

Black chicken feathers generated in large amount from poultry and slaughter houses are highly recalcitrant to microbial degradation due to their tough structural nature. A novel keratinolytic bacterium that possessed high affinity for black feather was isolated from chicken manure and identified as Pseudochrobactrum sp. IY-BUK1. Keratinase and feather soluble protein were effectively produced by the free living cells of the bacterium in media containing only black feathers and a mixture of equal amount of black-, brown- and white-coloured feathers. Complete degradation of 5 g/L of black feathers was completed in 3 days following optimisation of physico-chemical conditions. However, the bacterium selectively completed the degradation of black feather in a medium containing mixture of feathers in 144 h leaving behind approximately 33% and 45% of brown and white feathers in the medium respectively. Gellan gum-immobilised cells of strain IY-BUK1 enhanced the keratinase production by about 150% and were used repeatedly for ten cycles to degrade 5 g/L of black feather in a semi continuous fermentation of 18 h per cycle with enhanced and stable production of soluble protein. The study demonstrated the potential use of Pseudochrobactrum sp. IY-BUK1 not only in biodegradation of highly recalcitrant black feathers, but also in producing keratinase enzymes and valuable soluble proteins for possible industrial usage.

Assessment of Occupational Safety and Hygiene Perception among Afro-Caribbean Hair Salon Operators in Manchester, United Kingdom.

Because of exposure to a number of potential health hazards within the work environment, hairstylists experience occupational diseases that include occupational asthma, skin conditions and musculoskeletal diseases. The paucity of studies assessing occupational safety and hygiene management among Afro-Caribbean hair salon operators in the UK promoted the study. QualtricsTM was used to assess the participants' perception of exposure to hair products and their personal safety and hygiene knowledge, attitudes, awareness, and risk perceptions at work. In five salons, indoor air quality was monitored over one working week for selected environmental pollutants: temperature, humidity, CO, CO2 and Total Volatile Organic Compounds (TVOCs) using a GrayWolf Direct Sense Indoor Air Quality-IAQ (IQ-610). The use of unflued gas heating to raise the indoor temperature was common among the salons' operators which explains the high carbon monoxide readings recorded. Itchy eyes and nose (44.4%) shoulder, neck and back pain (39.2%) were frequently reported. Age-stratified analysis of reported occupational ailments showed participants within an age bracket of 31-35 reported allergies (24%) and itchy eyes and nose (19.1%) as the most common of occupational ailments. Respiratory, skin and musculoskeletal symptoms ranked as major occupational ill-health experiences among the study population. The study outcome demonstrated that the type of activity and the hair products used play an important role in the level of pollutants in the working environment. The substitution of the more harmful hair products with safer alternatives is needed, as is the encouragement of health surveillance.