Radiographic and histologic assessment of osseointegration for surface-treated titanium dental implants: An experimental study in dogs.

Background: Modifying the dental implant's surface texture enhances its biological response to the bone and implant‒tissue interface, resulting in the successful support of forces. This study assessed the impact of sandblasting, sandblasting plus acid etching, Er,Cr:YSGG laser, and propolis implant surface treatments and occlusal load on the osseointegration of titanium dental implants in dogs. Methods: Seventy-two titanium dental implants with a diameter of 4 mm and a length of 10 mm were divided into four groups according to implant surface modifications (n=18 for each group): group A: sandblasting plus acid etching, group B: sandblasting with Al2 O3, group C: Er,Cr:YSGG laser, group D: propolis coating. Twenty-four local breed male dogs were used. Premolar teeth (P1, P2, and P3) were extracted on the left side of the mandible, and after 12 weeks of bone healing, implants were unilaterally installed. The osseointegration at three study times from implant installation (14, 90, and 180 days) was evaluated. The dog jaws were scanned using an intraoral scanner for the virtual design of screw-retained three-unit crowns after 90 days of osseointegration. Final radiographs were taken before the animals were sacrificed at 14, 90, and 180 days, and the histological analysis was performed. Results: Radiographic analysis showed new bone formation (NBF) along and in contact with the implant surface of the treated groups. The histological analysis after 14 days in groups A and B revealed a uniform and ongoing pattern of bone growth and many osteoblasts with few osteocytes within lacunae in new bone trabeculae. Group C showed an increase in the number of osteoblasts lining thin bone trabeculae. Group D showed a generative power concerning bone. At 90 days, there was increased bone ingrowth, and the new bone matured in all the treated implant groups. At 180 days, dense mature bone apposition was in direct contact with delayed-loaded implant surfaces. Conclusion: A radiographic examination revealed that surface modification significantly impacted osseointegration, with a strong bond between the implant surface and the surrounding bone. The histological sections at the 14-day interval revealed obvious bone remodeling activity, especially in sandblasting plus acid etching and sandblasting-modified implant surface groups. At the 90-day interval, bone ingrowth had increased, and the new bone became mature, especially in sandblasting and propolis surface modification groups. After 180 days of the delayed-loaded implant osseointegration, differences were observed between different implant-treated groups with a remarkable remodeling of the bone, especially in the propolis coating group.

Effects of restraint stress and surface treatments on the stability of titanium dental implant osseointegration in dogs: An in vivo comparative study.

Objectives: This study was aimed at assessing the effects of restraint stress and sandblasting; sandblasting with acid etching; Er-Cr: YSGG laser treatment; and propolis coating of implant surfaces on the implant stability quotient (ISQ) of grade 4 titanium dental implant osseointegration in model dogs. Methods: A total of forty-eight CPTi dental implants were divided into four groups according to surface treatment: group A: sandblasting with acid etching; group B: sandblasting with Al2O3; group C: Er-Cr: YSGG laser; and group D: propolis coating. Sixteen male dogs of local breed,1-1.5 years of age, weighing 22 ± 3 kg, were divided into two main groups (n-8 dogs each): a non-stressed group (group I) and stressed group (group II). Each of these groups is further divided into four implant groups per surface treatment, A, B, C, and D (two dogs per implant group), each dog has three implants. The ISQ was tested at 0 (baseline), 14, and 90 days with a noninvasive EasyCheck® device. Serum cortisol in the stressed and non-stressed groups was analyzed at 0, 15, 30, 45, 60, 75, and 90 days with a canine cortisol ELISA kit. The data were statistically analyzed with one-way analysis of variance and Tukey's post hoc test at a 0.05 level of significance. Results: For implant stability quotient (ISQ), sandblasting in the non-stressed (group I) had the highest mean value (88.0) at 90-days, whereas sandblasting with acid etching at 14-days had the lowest mean value (82.6). Sandblasting in the stressed (group II) had the highest mean value (88.3) at 90-days, whereas the laser surface treatment had the lowest mean value (72.00) at 14-days. Serum cortisol (ng/µl) at 90 days (143.10 and 195.33 for non-stressed and stressed groups respectively), was significantly higher than other time intervals (P < 0.05). Conclusions: The ISQ was dependent on surface treatment, and was higher with sandblasting than the other treatments in the stressed and non-stressed groups at 90 days. For all surface-treated implants, the stressed group had significantly higher serum cortisol levels than the non-stressed group.

Impact of Er,Cr:YSGG Laser, Sandblast, and Acid Etching Surface Modification on Surface Topography of Biodental Titanium Implants.

Introduction: Several techniques have been used to modify the surface of commercially pure titanium (CPTi) implants to improve osseointegration using lasers, sandblasts, sandblasts with acid etching, and other modalities. For implant-osseointegration, surface features like chemical composition of a surface, topography, and surface energy are essential. The present comparative study aimed to compare the impact of Er,Cr:YSGG laser, sandblasting, and acid etching implant surface modifications on the surface topography, roughness, and element chemical composition of the Ti dental implant. Methods: Thirty CPTi dental implants were divided into three groups according to the surface modification (n=10 for each group): Group A: Sandblasting with acid etching (SLActive), group B: Sandblasting, and group C: Er,Cr:YSGG laser surface modifications. The modified surfaces were analyzed using scanning electron microscopy (SEM), profilometer, and energy dispersive x-ray spectrometry (EDS). Results: One-way analysis of variance (ANOVA) showed that there were significant differences in the mean values of average roughness (Ra) of the three groups (P<0.05). Tukey's post hoc test showed that the average roughness (Ra) of laser-surface modification (group C) of the implant had the highest mean value (2.30 µm) among the different groups, while sandblasted surface modification (group B) of the implant had the lowest mean value (1.39 µm). The SLActive (group A) sandblast with acid etching had a mean value of 1.63 µm. SEM analysis showed that significantly modified surface topographies and different element concentrations were found within all modified groups. Conclusion: The Er,Cr:YSGG laser irradiation increased the implant surface roughness value after implant surface modification, compared to sandblasts and sandblasts with acid etching application. The observations for the SEM-EDS analysis revealed several elements with different concentrations, which were affected by the physical-chemical characteristics of the surface modification techniques. The SEM analysis showed a significant modification in implant surface topographies of the tested groups.

Shear Bond Strength of Two Repair Systems to Zirconia Ceramic by Different Surface Treatments.

Introduction: Intraoral repair has been suggested as a treatment option to repair the chipping or fracture of veneered zirconia; the success of the procedure is mainly determined by the bonding between zirconia and composite resin. The present study aimed to investigate and compare the shear bond strength (SBS) of two intraoral repair systems to zirconia ceramic treated with a laser or air-abrasion surface modification. Methods: Ninety tube-shaped samples (diameter of 10 mm and height of 4 mm) were divided into three main groups: Group I (zirconia 100%, n=30); Group II (veneer ceramic 100% n=30); Group III (zirconia with a veneer ceramic n=30). Each main group was subdivided into two subgroups (n=15): Subgroup A: samples repaired with Ceramic Repair N; Subgroup B: samples repaired with the Cimara Repair System. The subgroup samples were further subdivided based on the treated surface (n=5 samples): Control (no surface modification), Er,Cr:YSGG laser surface modification, and air-abrasion surface modification. The SBS was employed using a universal testing machine. The mode of failure was observed using a stereomicroscope. Results: Significant differences were observed in the mean SBS values between the different surface modifications (P˂0.05). Tukey's post hoc test showed that the air-abrasion surface modification of the veneer ceramic repaired with the Ceramic repair N system had the highest mean value (13.74 MPa) among the different groups, while no surface modification of zirconia repaired with the Cimara repair system had the lowest mean value (2.84 MPa). The control group (no surface modification) had the lowest mean value among all the treated groups. Conclusion: The SBS is surface modification-dependent, and higher SBS is obtained by air-abrasion than Er, Cr:YSGG laser surface modifications with the selected parameters. The Ceramic repair N system had significantly higher SBS for all surface-treated substrates than the Cimara repair system.