RESUMO
AIM: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. METHODS AND RESULTS: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17-aadA5, dfrA1-aadA1, dfrA12-orf5-aadA2 and bla(OXA-30)-aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. CONCLUSIONS: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC.
Assuntos
Farmacorresistência Bacteriana Múltipla/genética , Infecções por Escherichia coli/epidemiologia , Integrons/genética , Infecções Urinárias/epidemiologia , Escherichia coli Uropatogênica/efeitos dos fármacos , Antibacterianos/farmacologia , DNA Bacteriano/genética , Desoxirribonucleases de Sítio Específico do Tipo II , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/genética , Humanos , Líbano/epidemiologia , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Prevalência , Análise de Sequência de DNA , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica/genética , Escherichia coli Uropatogênica/isolamento & purificaçãoRESUMO
Staphylococcus aureus is an important human pathogen and is a growing public health concern. In this study, 130 S. aureus, 93 methicillin-resistant S. aureus (MRSA) and 37 methicillin-sensitive S. aureus (MSSA), clinical isolates recovered from Lebanon were typed by protein A gene (spa) sequencing and multi-locus sequence typing (MLST). Forty-eight different spa types were identified and clustered into 30 different groups. MLST revealed 10 sequence types (STs) among the isolates. There were eight major MRSA clones defined as isolates with the same ST and the same SCCmec type. The majority of the PVL-positive isolates (53%) were ST80-MRSA-IVc. Systematic surveillance of both hospital and community isolates in Lebanon together with measures designed to limit the spread are required.
Assuntos
Técnicas de Tipagem Bacteriana , Impressões Digitais de DNA , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/classificação , Staphylococcus aureus/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Toxinas Bacterianas/genética , Criança , Pré-Escolar , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , Exotoxinas/genética , Feminino , Genótipo , Humanos , Lactente , Recém-Nascido , Líbano/epidemiologia , Leucocidinas/genética , Masculino , Resistência a Meticilina , Pessoa de Meia-Idade , Epidemiologia Molecular , Análise de Sequência de DNA , Proteína Estafilocócica A/genética , Staphylococcus aureus/isolamento & purificação , Adulto JovemRESUMO
Escherichia coli is the most common causative agent of recurrent, uncomplicated urinary tract infections. Triplex pCR and the disc diffusion method were used to determine and correlate among the phylogenetic group, virulence determinants and antimicrobial resistance in 100 uropathogens. Group B2 represented 58% of the tested isolates with no isolates belonging to group B1. Isolates with the highest percentage of susceptibility to all antimicrobial agents used were within the B2 phylogenetic groups. 38% of the tested population were resistant to trimethoprim/sulfamethoxazole, 29% to ciprofloxacin and only 8% to nitrofurantoin. The majority of the isolates resistant to trimethoprim/sulfamethoxazole were from group B2 (52.7%), with 45% being positive to the three tested virulence determinants (efu, fbp and picU). These findings suggest that although virulence and antimicrobial resistance are mutually exclusive in Escherichia coli clinical isolates, the relationship between virulence and resistance to antimicrobial agents can vary according to the particular resistance phenotype.
Assuntos
Anti-Infecciosos Urinários/farmacologia , Farmacorresistência Bacteriana/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Fatores de Virulência/genética , Humanos , Líbano , Testes de Sensibilidade Microbiana , Filogenia , Reação em Cadeia da Polimerase , Virulência/genéticaRESUMO
Antibiotic-resistant bacteria were common in samples collected from an intermittent water distribution system in Lebanon. Multiply-resistant isolates were also present and most commonly to amoxycillin, cephalexin and sulfamethoxazole/trimethoprim. The aminoglycosides (amikacin, gentamicin and kanamycin) were the most effective, with almost all tested strains showing susceptibility to these antimicrobial agents. Both the Biolog GN MicroPlates and the API 20E strips can be used for the identification of coliform bacteria isolated from potable water, but the outcome of the identification should be viewed with caution. 51% of isolates were assigned similar identities by both the Biolog MicroPlates and the API 20E strips. The similarity at the species level was lower (33%) compared to that at the genus level (67%). The identification of Escherichia coli strains, which represented 30% of all tested organisms, showed 95% similarity in the assigned genus and species using both identification schemes.
Assuntos
Resistência a Múltiplos Medicamentos , Enterobacteriaceae/efeitos dos fármacos , Enterobacteriaceae/patogenicidade , Microbiologia da Água , Abastecimento de Água , Resistência Microbiana a Medicamentos , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Escherichia coli/patogenicidade , Incidência , Testes de Sensibilidade MicrobianaRESUMO
A controlled study was conducted in Lebanon over a period of 12 months to determine bacterial regrowth in a small network supplying the Beirut suburb of Naccache that had a population of about 3,000. The residential area, which is fed by gravity, is supplied twice a week with chlorinated water from two artesian wells of a confined aquifer. A significant correlation was detected between the turbidity and the levels of heterotrophic plate count bacteria (HPC) in the samples from the distribution network as well as from the artesian wells. However, a negative significant correlation was found between the temperature and the HPC count in the samples collected from the source. A statistically significant increase in counts, possibly due to regrowth, was repeatedly established between two sampling points lying on a straight distribution line but 1 km apart. Faecal coliforms were detected in the source water but none in the network except during a pipe breakage incident with confirmed Escherichia coli reaching 40 CFU/100 mL. However, coliforms such as Citrobacter freundii, Enterobacter agglomerans, E. cloacae and E. skazakii were repeatedly isolated from the network, mainly due to inadequate chlorination. A second controlled study was conducted to determine the effect of storage on the microbial quality of household storage tanks (500 L), which were of two main types - galvanized cast iron and black polyethylene. The mean bacterial count increased significantly after 7 d storage in both tank types. A significant difference was found in the mean HPC/mL between the winter and the summer. Highest counts were found April-June although the maximum temperature was reported later in the summer. A positive correlation was established between the HPC/mL and pH, temperature and storage time.
Assuntos
Bactérias , Microbiologia da Água , Abastecimento de Água , Líbano , Dinâmica Populacional , Controle de Qualidade , Fatores de Tempo , População Urbana , Purificação da ÁguaRESUMO
Recovery of Campylobacter was attempted from 281 consecutive non selected out-patients diarrheic stools, 150 individual ceca collected from meat chicken breeder farms and 31 slaughtered marketed chicken obtained from shops in Lebanon. Campylobacter isolates were recovered from 2 (0.7%) human stool specimens, 34 (22.7%) chicken ceca and 3 (9.7%) raw chicken carcasses. Speciation of these isolates revealed 2 C. jejuni from humans diarrheic stools, 16 C. coli, 10 C. jejuni, 3 C. fetus, 2 C. fennelliae (Helicobacter fennelliae, new taxon), 2 C. upsaliensis, 1 C. cryaerophila (Archobacter cryaerophilus, new taxon) from chicken ceca and 2 C. coli and 1 C. fennelliae (H. fennelliae) from raw chicken carcasses. Antimicrobial susceptibility testing of the different isolates against 9 antimicrobial agents was performed using the E-test. Overall, most isolates showed high to moderate susceptibility to gentamicin (97%), amoxicillin/clavulanate (95%), clindamycin (77%), chloramphenicol (77%), and ampicillin (69%). Lower susceptibility were observed against tetracycline (49%), erythromycin (47%), ciprofloxacin (39%), and norfloxacin (36%). This overall susceptibility profile generally applied to C. coli and C. jejuni, as well, although C. coli mostly showed higher susceptibility than C. jejuni. beta-lactamase production was detected in 59% of all the isolates, being higher in C. coli (72%) than C. jejuni (33%). Whole cell protein profile analysis of 18 C. coli and 12 C. jejuni by SDS-PAGE revealed 6 different patterns. In both species, major variations existed in the region between mol wt 45-60 and all protein profiles were dominated by the presence of 5 major bands of mol wt: 61 (doublet), 45, 31 and an approximate 24. Differences in banding patterns within and between both species indicated diversity and heterogeneity of strains. This study shows that despite high prevalence and diversity of strains in chicken, Campylobacter in Lebanon is rare in human diarrheic stools compared to Salmonella (3.2%) and Shigella (1.4%).