RESUMO
BACKGROUND: The cryopreservation of the sperm of the depik fish, Rasbora tawarensis, has previously been developed. However, the quality of the sperm post cryopreservation was not satisfactory and might be improved through the application of antioxidants. OBJECTIVE: To determine the most suitable antioxidant for the cryopreservation of the depik fish spermatozoa. MATERIALS AND METHODS: A completely randomized design with a non-factorial experiment was used and the tested antioxidants were glutathione, beta-carotene, ascorbic acid, and butylated hydroxytoluene (BHT) at 6 % concentrations. All treatments had three replications. The sperms were collected from 10 male fishes and diluted with Ringer solution in a ratio of 1: 20 (v/v, sperm: Ringer solution). Then 5% DMSO and 5 % egg yolk were added to the diluted sperms. Furthermore, 6 % of the tested antioxidants were added to the diluents, and then, cryopreservation was carried out in liquid nitrogen for 14 days. RESULTS: The ANOVA test showed that the application of antioxidants significantly affected the sperm motility, fertility, and hatching rates of the eggs (P < 0.05). Furthermore, the antioxidants also protected the sperm cells during cryopreservation, with glutathione being the best antioxidant. CONCLUSION: The application of antioxidants during the cryopreservation of depik fish sperm had a significant effect on motility, fertility and hatchability of eggs post-cryo. Furthermore, glutathione was the most suitable antioxidant. doi.org/10.54680/fr23110110312.
Assuntos
Cipriniformes , Preservação do Sêmen , Animais , Masculino , Antioxidantes/farmacologia , Solução de Ringer , Criopreservação/veterinária , Sêmen , Motilidade dos Espermatozoides , Preservação do Sêmen/veterinária , Espermatozoides , Glutationa/farmacologia , Crioprotetores/farmacologiaRESUMO
The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and 3 replications. The Seurukan fish sperm was diluted in extender with a ratio of 1:20 (sperm: extender), then glutathione was added at a concentration of 0, 10, 20, 30, 40 and 50â¯â¯mgâ¯L-1. Next, the sperm thawed at 39-40⯰C for 3â¯min and mixed with 100 eggs which were randomly selected. The success of the fertilized egg was observed 6â¯h after fertilization, while the hatching rate was examined 60â¯h after fertilization. The ANOVA test showed that the addition of glutathione significant affected the sperms motility, fertility and hatching rate of seurukan fish Osteochilus vittatus eggs (Pâ¯<â¯0.05). By segregation the fresh sperm, a higher sperm motility rate was recorded with an addition of 30â¯mgL-1 of glutathione (63.00⯱â¯5.89), however, this value was not significantly different from using concentration of 10 and 20â¯â¯mgâ¯L-1. A higher fertilization rate was produced using glutathione concentration of 50â¯â¯mgâ¯L-1 (51.33⯱â¯17.01); however, it was also not significantly different from the concentration of 20, 30 and 40â¯mgL-1. In addition, a higher hatching rate was also recorded using glutathione concentration of 50â¯mgâ¯L-1 (40.33⯱â¯12.89), this value was not significantly different from using 40â¯mgL-1 glutathione. Hence, a conclusion was drawn that the optimum concentration of glutathione is 40â¯mgâ¯L-1 of diluent.