RESUMO
Toads of the Bufo peltocephalus Group (Anura: Bufonidae) occur throughout the Greater Antilles (Cuba to the Virgin Islands), a geographic region of relatively high endemicity. Previous morphological and immunological studies suggested that the West Indian toads are a monophyletic lineage derived from Neotropical Bufo but were unable to clarify relationships within the group. We examined the evolutionary relationships and biogeography of this group of frogs by collecting approximately 2 kb of mitochondrial DNA sequence data from eight West Indian species and selected non-West Indian species from the New World and the Old World. Our analyses support the monophyly of native West Indian toads and a New World origin for the group. Relationships among the West Indian species are less certain, but a Cuban lineage is defined in most analyses.
Assuntos
Bufonidae/genética , DNA Mitocondrial/genética , Filogenia , Animais , Bufonidae/classificação , Grupo dos Citocromos b/genética , DNA Mitocondrial/química , Evolução Molecular , Geografia , Dados de Sequência Molecular , RNA Ribossômico/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNAAssuntos
Evolução Biológica , Animais , DNA Mitocondrial/genética , Peixes , Humanos , Filogenia , RNA/genética , VertebradosRESUMO
The quantitative immunological technique of microcomplement fixation was used to examine serum albumin evolution among members of the order Crocodylia. The cross-reactivity of the albumin antisera and antigens employed in this study had been examined previously using the qualitative technique of immunodiffusion. The phylogenetic conclusions derived from these two data sets are highly congruent, including support of the families Alligatoridae and Crocodylidae, with the placement of Gavialis as the sister taxon of Tomistoma. Both methods provide similar information on the relative amounts of amino acid sequence divergence between albumin molecules; however, the data obtained from microcomplement fixation comparisons are more discriminating than those derived from immunodiffusion. The estimated divergence times within the Crocodylia derived from the fossil record are examined in light of divergence times predicted by the microcomplement fixation-based albumin clock. The traditional phylogenetic placement of Gavialis outside the remaining extant crocodilians is inconsistent with all molecular data sets and we suggest that a careful reexamination of both the extant and the fossil morphological data is warranted.
Assuntos
Jacarés e Crocodilos/genética , Jacarés e Crocodilos/imunologia , Evolução Biológica , Jacarés e Crocodilos/classificação , Animais , Variação Antigênica , Testes de Fixação de Complemento , Imunodifusão , Técnicas Imunológicas , Filogenia , Albumina Sérica/genética , Albumina Sérica/imunologia , Especificidade da EspécieRESUMO
The geological association of the Greater Antilles with North and South America in the late Cretaceous led to the hypothesis that the present Antillean biota reflects those ancient land connections. Molecular data from diverse West Indian amphibians and reptiles and their mainland relatives support a more recent derivation of the Antillean vertebrate fauna by overwater dispersal. The catastrophic bolide impact in the Caribbean region at the close of the Cretaceous provides a proximate cause for the absence of an ancient West Indian biota.
