Recent advances in carbon quantum dots for gene delivery: A comprehensive review.

Gene therapy is a revolutionary technology in healthcare that provides novel therapeutic options and has immense potential in addressing genetic illnesses, malignancies, and viral infections. Nevertheless, other obstacles still need to be addressed regarding safety, ethical implications, and technological enhancement. Nanotechnology and gene therapy fields have shown significant promise in transforming medical treatments by improving accuracy, effectiveness, and personalization. This review assesses the possible uses of gene therapy, its obstacles, and future research areas, specifically emphasizing the creative combination of gene therapy and nanotechnology. Nanotechnology is essential for gene delivery as it allows for the development of nano-scale carriers, such as carbon quantum dots (CQDs), which may effectively transport therapeutic genes into specific cells. CQDs exhibit distinctive physicochemical characteristics such as small size, excellent stability, and minimal toxicity, which render them highly favorable for gene therapy applications. The objective of this study is to review and describe the current advancements in the utilization of CQDs for gene delivery. Additionally, it intends to assess existing research, explore novel applications, and identify future opportunities and obstacles. This study offers a thorough summary of the current state and future possibilities of using CQDs for gene delivery. Combining recent research findings highlights the potential of CQDs to revolutionize gene therapy and its delivery methods.

Microextraction with smartphone detection of thiocyanate in saliva of tobacco smokers using paper-based analytical method.

This study presents a novel, cost-effective approach involving spectrophotometric and smartphone paper-based (SPB) methods and a distinctive salting-out air-assisted dispersive microextraction procedure to quantify thiocyanate in saliva samples. The method relies on the inhibitory effect of thiocyanate on quinoneimine dye formation during the Emerson reaction with sodium hypochlorite. Spectrophotometry quantifies the extracted dye by monitoring quinoneimine color intensity reduction at 525 nm. In the SPB method, extracted dye is applied to a paper strip, a smartphone captures the colored paper, and an application analyzes red, green, and blue components. All analyte determination and extraction variables were explored. Both methods exhibit good linearity (10-100 µg/L) with a coefficient of determination of 0.9991 and a limit of detection of 7.5 µg/L for the spectrophotometric method, and a coefficient of determination of 0.9988 and a limit of detection of 8.8 µg/L for the SPB method. The calculated values for the enrichment factor and extraction recovery of the developed extraction methodology were 46% and 93%, respectively. The methods detect thiocyanate in saliva samples, producing results comparable to a validated method.

Evaluating the health risks of heavy metal pollution in dust storms in the city of Erbil in the Kurdistan region of Iraq.

This study examined the heavy metal content in dust storm samples from Erbil, Iraq, along with four other locations. Using ICP-MS, Cd, Ni, Cr, Hg, Pb, Zn, Mn, Cu, Co, Fe and As were determined. The health risks due to exposure to these metals through ingestion, inhalation and dermal contact were assessed for both adults and children. Non-carcinogenic risks were evaluated using the hazard quotient (HQ) and hazard index (HI). Children faced a cumulative risk with HQ > 0.2 for As and Cr and HI > 1. The carcinogenic risk was measured using the carcinogenic risk factor (CRF), which fell below 10-6, indicating low cancer risk. However, children had a higher cancer risk (10-4 to 10-6) for As. The pollution indices revealed varying pollution levels from unpolluted to moderately polluted in the studied areas. Overall, this study highlights potential health risks associated with heavy metal exposure during dust storms, particularly for children, and emphasises the importance of addressing these concerns to safeguard public health.

New spectrophotometric and smartphone-based colorimetric methods for determination of atenolol in pharmaceutical formulations.

Novel spectrophotometric and smartphone-based colorimetric methods were developed and validated for the estimation of atenolol (ATE) in pharmaceutical formulations. The measurement procedure is based on the de-diazotization reaction, in which ATE is able to inhibit the diazotized sulfanilic acid from reacting with 8-hydroxy quinoline (8-HQ) in a basic medium. As a result, the formation of red-orange color azo-dye is hindered, and the color intensity is decreased proportionally to concentration of ATE. In spectrophotometric method the azo-dye color fate was monitored at 495 nm. While in smartphone-based colorimetric (SBC) method the captured image in the design processed by RGB App and transferred to the absorbance. The reactant concentrations were optimized using a central composite design (CCD) and response surface method. The methods exhibit good linearity in the 8.0 to 60.0 µg mL-1 range with no significant effect of interferences. The spectrophotometric method yields a linear equation with a slope of 0.0187 (R2 = 0.9993), a limit of detection (LOD) of 1.28 µg mL-1, and a limit of quantification (LOQ) of 4.28 µg mL-1. On the other hand, the smartphone-based colorimetric (SBC) method demonstrates a linear equation with a slope of 0.0127 (R2 = 0.9965), an LOD of 2.13 µg mL-1, and an LOQ of 7.09 µg mL-1. Analyzing ATE in pharmaceutical tablets was utilized to validate the applicability of the developed methods, and the results were statistically compared with those obtained by the HPLC method using the t-test and F-test.

Newly synthesized Fe-doped CDs for colorimetric and fluorometric nanozyme-based levodopa sensing.

A simple single-pot hydrothermal method was used to fabricate a Fe, N, and S co-doped carbon dots (Fe-CDs) nanozyme using ferric chloride and sunset yellow as precursors. The fabricated Fe-CDs exhibited intense green fluorescence at 460 nm with excitation-independent properties and a high quantum yield of 40.23%. This nanozyme mimics peroxidase by catalyzing the oxidation of tetramethylbenzidine (TMB) by H2 O2 to yield a blue-coloured TMBox product at 652 nm. Dual detection methods were established for determining levodopa (l-dopa) by taking advantage of the high nanozyme activity and the distinct fluorescence aspect. Both determination methods are based on the oxidation of l-dopa by H2 O2 in the presence of Fe-CDs and fading of the blue colour of the TMBox . The colorimetric method monitors the amount of colour fading of TMBox . In the fluorometric method, the formed blue TMBox absorbs the emission light of the Fe-CDs; when l-dopa is present, this effect decreases and the intensity of the emission light increases. The nanozyme-based detection procedures exhibit good linearity in the ranges 2.17 × 10-3 to 34.78 × 10-3 mM [limit of detection (LOD) = 0.84 × 10-3 mM] and 0.85 × 103 to 16.95 × 103 nM (LOD = 0.102 × 103 nM) for colorimetric and fluorometric methods, respectively.