1.
Nihon Naika Gakkai Zasshi
; 102(1): 143-6, 2013 Jan 10.
Artigo
em Japonês
| MEDLINE
| ID: mdl-23777125
2.
Anal Chem
; 83(24): 9197-200, 2011 Dec 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22070407
RESUMO
Because current homogeneous immunoassays show some limitations, particularly low sensitivity, we developed a new immunoassay to overcome these limitations. The approach was based on magnetic nanoparticles with a thermoresponsive polymer layer, a negatively charged polymer, and streptavidin-biotin-based antibody-antigen detection and yielded higher sensitivity than commonly used heterogeneous immunoassays. Because no special equipment is needed, it can be applied to currently available absorbance-based systems for high-throughput assays.