Fimbriae and determination of host species specificity of Bordetella bronchiseptica.

A monoclonal antibody, designated CF8 and prepared against fimbrial protein enrichments of Bordetella bronchiseptica 110H, was determined by immunogold electron microscopy to bind to some but not all fimbrial filaments on intact bacterial cells. Comparison of the reactivity of this antibody with that of monoclonal antibody BPF2, which is specific for Bordetella pertussis serotype 2 fimbriae, indicated that CF8 recognizes an epitope similar to that recognized by BPF2. By Western blot (immunoblot), it was determined that monoclonal antibody CF8 does not react with proteins denatured by treatment with sodium dodecyl sulfate and beta-mercaptoethanol and by boiling for 5 min but that it does recognize fimbrial proteins in their native, nondenatured state. This antibody was used to compare fimbriae between strains of B. bronchiseptica isolated from different species. Strains from pigs, dogs, guinea pigs, and four other species were compared by an enzyme immunoassay. Strains isolated from pigs were found to express significantly more CF8-reactive and B. pertussis serotype 2 cross-reactive fimbriae than strains isolated from guinea pigs. Strains from dogs were more variable in reactivity than those from pigs or guinea pigs. The reactivity with antifimbrial monoclonal antibody CF8 did not correlate with enzyme electromorphotype but did correlate with the host species, suggesting a role for fimbriae in the determination of host species specificity of B. bronchiseptica.

Evaluation of extracorporeal perfusion catheters using a standardized measurement technique--the M-number.

Cannulas used for extracorporeal circulating devices are made in many shapes and sizes. Side hole placement, length, and wall thickness can make the pressure-flow characteristics of catheters with similar French sizes quite different in their actual performance. A single value, the M-number, has been developed that describes the pressure-flow characteristics of an individual cannula. This number can be used to compare catheters and assess their potential utility for given bypass requirements. The M-numbers for commonly used catheters that are placed by extrathoracic dissections are determined. Clinical situations are presented that demonstrate applications of the M-number.