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1.
J Virol ; 80(11): 5494-8, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16699029

RESUMO

Upon entering a cell, alphaherpesvirus capsids are transported toward the minus ends of microtubules and ultimately deposit virus DNA within the host nucleus. The virus proteins that mediate this centripetal transport are unknown but are expected to be either viral tegument proteins, which are a group of capsid-associated proteins, or a surface component of the capsid itself. Starting with derivatives of pseudorabies virus that encode a fluorescent protein fused to a structural component of the virus, we have made a collection of 12 mutant viruses that lack either the VP26 capsid protein or an individual tegument protein. Using live-cell fluorescence microscopy, we tracked individual virus particles in axons following infection of primary sensory neurons. Quantitative analysis of the VP26-null virus indicates that this protein plays no observable role in capsid transport. Furthermore, viruses lacking tegument proteins that are nonessential for virus propagation in cell culture were also competent for axonal transport. These results indicate that a protein essential for viral propagation mediates transport of the capsid to the nucleus.


Assuntos
Proteínas do Capsídeo/metabolismo , Proteínas de Transporte/metabolismo , Herpesviridae/fisiologia , Proteínas Nucleares/química , Animais , Transporte Biológico , Proteínas do Capsídeo/química , Proteínas de Transporte/química , Linhagem Celular , Núcleo Celular/metabolismo , Microtúbulos/metabolismo , Suínos
2.
J Virol ; 80(1): 201-9, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16352544

RESUMO

Transport of capsids in cells is critical to alphaherpesvirus infection and pathogenesis; however, viral factors required for transport have yet to be identified. Here we provide a detailed examination of capsid dynamics during the egress phase of infection in Vero cells infected with pseudorabies virus. We demonstrate that the VP1/2 tegument protein is required for processive microtubule-based transport of capsids in the cytoplasm. A second tegument protein that binds to VP1/2, UL37, was necessary for wild-type transport but was not essential for this process. Both proteins were also required for efficient nuclear egress of capsids to the cytoplasm.


Assuntos
Proteínas do Capsídeo/metabolismo , Herpesvirus Suídeo 1/fisiologia , Proteínas Virais de Fusão/fisiologia , Proteínas Estruturais Virais/fisiologia , Montagem de Vírus/fisiologia , Animais , Chlorocebus aethiops , Transporte Proteico , Células Vero , Vírion/fisiologia
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