Comparative environmental and economic life cycle assessment of phytoremediation of dredged sediment using Arundo Donax, integrated with biomass to bioenergy valorization chain.

The economic and environmental life cycle assessment (LCA) was integrated into a laboratory-based experiment to evaluate the feasibility and sustainability of phytoremediation of chloride-rich marine dredged sediment, using perennial reed Arundo Donax along with biomass valorization. As a prerequisite for life cycle assessments, a baseline mathematical model was developed to estimate the yields of biomass to bioenergy valorization chain including the estimation of biomass yield per m3 sediment, bioenergy yields from valorization schemes, expected green electricity yield, and the phytoremediation time frame. This mathematical model was applied to develop a parametric life cycle inventory for two scenarios of sediment phytoremediation separately or integrated with biomass valorization, for LCA and further sensitivity and uncertainty analysis. Comparative LCA unveiled that the cost and environmental impacts of annual phytoremediation of 1m3 sediment alone or integrated with biomass valorization are much inferior to the corresponding sediment landfill as the inevitable alternative approach for sediment management. With the chloride bioaccumulation capacity of 9940 mg per kg dry biomass of A. donax, the phytoremediation of sediment with chloride concentration higher than 1650 mg/kg may not be achievable in a realistic time frame. Due to the importance of considering sediment depth and the effectiveness of the plant rooting system in estimating the performance of phytoremediation and the time frame, the volume of sediment (1m3) is a more appropriate functional unit than the surface area (ha) for LCA studies of phytoremediation. In addition, considering the volume of sediment as a functional unit retains comparability to other valorization scenarios such as sediment incorporation in cementitious matrices and management scenarios such as landfill, which are generally expressed on a volume or mass basis. Integrating biomass-derived bioenergy production into phytoremediation could offer local and global benefits in terms of economy and environment mainly due to carbon sequestration and avoiding fossil-based fuels.

Environmental, economic and experimental assessment of the valorization of dredged sediment through sand substitution in concrete.

The integrated life cycle assessment (LCA), life cycle cost assessment (LCC) and laboratory-based experimental assessment were applied to provide insight for early stage decision-making on the valorization of the dredged sediments. The objective was to find a viable and sustainable solution for the valorization of the dredged sediment in concrete, holding up a certain level of standard concrete performance without compromising in terms of economy and environment. For the sensitivity analysis, parametric life cycle inventories were developed to assess the sensitivity of environmental and economic costs to the rate of sand substitution by sediment, as well as the variations in the concrete components. The workability of fresh concrete and the compressive strength of hardened concrete at 28 days were assigned as the quality indicators to evaluate the influence of sand substitution by sediment on the concrete performance. The compressive strength evaluation in the laboratory demonstrated that a maximum rate of sand substitution in concrete up to 40 % by predominantly sandy sediment could sustain the concrete strength class. However, LCA and LCC negated the rate of sand substitution by sediment higher than 20 %. The integrated environmental, economic, and experimental assessments demonstrated that the substitution of sand by predominantly fine sediment downgrades the strength class of concrete, even in the low rate of incorporation (10 %) and increases the environmental and economic costs. Inferred from the results, the maximum rate of sustainable sand substitution by sediment in concrete could be optimized through a compromise between the expected mechanical strength and workability of the concrete, the economic and environmental impacts of the superplasticiser and the sediment transport. Overall, integrating environmental and economic cost assessments into the laboratory-based assessment of the valorization scenarios would determine the threshold for the sustainable rate of incorporation of sediment in valorization scenarios.

The Marine Bacterium Shewanella woodyi Produces C8-HSL to Regulate Bioluminescence.

Quorum sensing (QS), a cell-to-cell communication system involved in the synchronization of bacterial behavior in a cell-density-dependent manner has been shown to control phenotypes such as luminescence, virulence, and biofilm formation. The marine strain, Shewanella woodyi MS32 has been identified as a luminous bacterium. Very little information is known on this bacterium, in particular if its luminescence and biofilm formation are controlled by QS. In this study, we have demonstrated that S. woodyi MS32 emits luminescence in planktonic and sessile conditions. The putative QS regulatory genes homologous to luxI and luxR identified in the S. woodyi MS32 genome, named swoI and swoR, are divergently transcribed and are not genetically linked to the lux operon in contrast with its closest parent Shewanella hanedai and with Aliivibrio fischeri. Interestingly, the phylogenetic analysis based on the SwoI and SwoR sequences shows that a separate horizontal gene transfer (HGT) occurred for the regulatory genes and for the lux operon. Functional analyses demonstrate that the swoI and swoR mutants were non-luminescent. Expression of lux genes was impaired in the QS regulatory mutants. N-octanoyl-L-homoserine lactone (C8-HSL) identified using liquid chromatography mass spectrometry in the wild-type strain (but not in ΔswoI) can induce S. woodyi luminescence. No significant difference has been detected between the wild-type and mutants on adhesion and biofilm formation in the conditions tested. Therefore, we have demonstrated that the luxCDABEG genes of S. woodyi MS32 are involved in luminescence emission and that the swoR/swoI genes, originated from a separate HGT, regulate luminescence through C8-HSL production.