[Studies on the chemical constituents of Epimedium brevicornum].

OBJECTIVE: To study the chemical constituents of Epimedium breviconum. METHODS: Compounds were purificated with various chromatographic techniques such as silica vacuum liquid chromatography and Sephadex LH-20 column chromatography. Their structures were elucidated by physico-chemical methods and spectral analysis. RESULTS: Ten compounds were obtained and indentified as (7R, 8S) 4,9-dihydroxyl-3,3'-dimethyoxyl-7, 8-dihydrobenzofunan-1'-propanolneolignan-9'-O-alpha-L-rhamnopyranoside (1), (7R, 8S, 8' R) 4, 4', 8', 9-tetrahydroxyl-3, 3'-dimethyoxyl-7, 9'-monoepoxylignan (2), (+)-cycloolivil (3), (3, 5, 7, 4')-tetrahydroxyl-8-isopentene group flavonoids-3-O-alpha-L-rhamnopyranoside (4), beta-sitosterol (5), p-hydroxybenzaldehyde (6), succinic acid (7) and p-hydroxyphenethyl (8). CONCLUSIONS: Compound 1 is isolated from this genus for the first time. Compounds 2,3,5 -8 are isolated from the plant for the first time.

[Determination of 5-hydroxymethyl furfuraldehyde in different processed products of polygonatum].

OBJECTIVE: By finding the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Polygonatum for different time, this provides basis for the technology of processing Polygonatum and quality standards of its prepared pieces. METHODS: The testing solution was acquired by ultrasonic extraction, HPLC was used to determine the contents. The chromatographic conditions: Agilent HC-C18 column (5 microm, 250 mm x 4.6 mm). The mobile phase: methanol-water (15:85) at the flow rate of 1 ml/min. The determination wave length was 284 nm. RESULTS: All kinds of processed Polygonatum had 5-Hydroxymethyl furfuraldethyde in different degrees, contents in Polygonatum kept relatively stable before 30 hours, but contents rose rapidly after 30 hours, contents decreased as continuing heating. CONCLUSION: The result can provide useful references to quality control and standard porcessing condition of Polygonatum.