Nitrogen retention potentials of magnesium oxide- and sepiolite-modified biochars and their impacts on bacterial distribution under nitrogen fertilization.

Mitigating the loss and negative impacts of reactive N from fertilized soils remains a global environmental challenge. To optimize N retention by biochar, bamboo and pig manure biochars were modified as MgO- and sepiolite-biochar composites and characterized. Novel soil application of the modified biochars and their raw forms were comparatively evaluated for N-retention in a fertilized soil leached for 90 days in a column experiment. Changes in N-cycling-related enzyme and bacterial structure were also reported after 90 days. Results revealed low leaching losses of NH4+, which reduced over time across all the treatments. However, while sole fertilizer (F) increased the initial and cumulative NO3- leached from the soil, the MgO-bamboo biochar (MgOBF) and sepiolite-bamboo biochar (SBF) treatments reduced leachate NO3- by 22.1 % and 10.5 % compared to raw bamboo biochar (BBF) treatment. However, 15.5 % more NO3- was leached from the MgO-pig manure biochar-treated soil (MgOPF) compared to its raw biochar treatment (PMBF) after 90 days. Dissolved organic N leached was reduced by 9.2 % and 0.5 % in MgOBF and SBF, as well as 15.4 % and 40.5 % in MgOPF and SPF compared to their respective raw forms. The total N of the biochars, adjustment of surface charges, cation exchange capacity, surface area, pore filling effects, and the formation of potential MgN precipitates on the modified-biochar surfaces regulated N leaching/retention. In addition, the modified biochar treatments reduced the hydrolysis of urea and stimulated some nitrate-reduction-related bacteria crucial for NO3- retention. Hence, unlike the raw biochar and MgOPF treatments, MgOBF, SBF, and SPF hold promise in mitigating inorganic-N losses from fertilized soils while improving the soil's chemical properties.

Influence of exposure history on the particle retention capacity and physiological responses of Euonymus japonicus Thunb. var. aurea-marginatus Hort.

Green plants in urban environments experience cyclical particulate matter stress. And this history of exhaust exposure may generate stress memory in plants, which may alter their subsequent response. Studies combining urban pollution characteristics and stress memory are limited. Therefore, we selected E. japonicus var. aurea-marginatus, a common urban greening tree species in the Yangtze River Delta, and conducted an experiment in three periods: the initial pollution period (S1: 28 days), the recovery period (R: 14 days) and the secondary pollution period (S2: 28 days). The experimental design consisted of an elevated pollution treatment (173 µg•cm-3) and an ambient control (34 µg•cm-3) with three replicates. In S2, the net total particle retention and saturated particle retention decreased by 11.5% and 19.3%, respectively, while PM10 and PM2.5 did not change significantly. E. japonicus var. aurea-marginatus exhibited recovery of chlorophyll levels, slower degradation of carotenoid, faster accumulation of ASA, lower accumulation of MDA, reduced activity of SOD under the second pollution period, and the period had a significant effect on the physiological indicators. Collectively, the effect of autoexhaust exposure history on the particle retention capacity of selected plant varied across particle sizes, and stress memory may confer plant resistance to recurrent exhaust pollution via combined regulations of physiological responses. Fine particles which pose a great risk to human health arise predominantly from vehicular traffic and energy production. So, E. japonicus tends to play a stabilising role in particle retention in industrial, traffic and residential areas.