RESUMO
Inadequate myocardial protection continues to be encountered despite improved methods of cardioplegia delivery. Although myocardial temperature is commonly monitored to assess the adequacy of cardioplegia delivery, its relationship to the metabolic status of the myocardium has not been investigated. We prospectively reviewed patients who underwent valvular heart surgery with blood (n = 47) or crystalloid (n = 48) cardioplegia and continuous measurement of intraoperative myocardial tissue pH and temperature. We previously demonstrated a high correlation (r = 0.99) between extracellular myocardial pH, levels of intracellular hydrogen ion concentration, and a lowering of tissue ATP during coronary occlusion. Clinically, optimal metabolic protection was defined as the absence of myocardial tissue acidosis during the period of aortic occlusion as quantified by a temperature-corrected integrated mean pH of 6.8 or greater, which has been shown to be predictive of a favorable postoperative outcome. Age, bypass time, myocardial temperature, myocardial tissue pH at the onset of aortic occlusion, cross-clamp time, and volume of cardioplegia were not significantly different between blood and crystalloid groups. Linear regression analysis demonstrated no significant correlation between mean myocardial tissue pH and the corresponding mean myocardial temperature in either group during aortic occlusion. There was also no correlation between the mean myocardial tissue pH and volume of cardioplegia delivered in both groups. These data demonstrate wide intercardiac and intracardiac variability in the degree of regional tissue acidosis encountered during of hypothermic cardioplegia. Cardioplegia delivery guided by measurement of myocardial temperature or by standardized protocol did not prevent the occurrence of tissue acidosis and thus, did not ensure optimal metabolic protection of the heart. In 95 patients undergoing valvular heart surgery with cold blood or crystalloid cardioplegia, there was no correlation between myocardial tissue pH and mycardial temperature or between myocardial tissue pH and volume of cardioplegia administered. Temperature is a poor indicator of the metabolic state of the myocardium.
Assuntos
Ponte de Artéria Coronária , Doenças das Valvas Cardíacas/cirurgia , Hipotermia Induzida , Monitorização Intraoperatória , Equilíbrio Ácido-Base/fisiologia , Idoso , Soluções Cardioplégicas/administração & dosagem , Ponte Cardiopulmonar , Feminino , Doenças das Valvas Cardíacas/fisiopatologia , Hemodinâmica/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Miocárdio/metabolismo , PrognósticoRESUMO
OBJECTIVES: Improving methods of donor heart preservation may permit prolonged storage and remote procurement of cardiac allografts. We hypothesized that continuous, sanguineous perfusion of the donor heart in the beating, working state may prolong myocardial preservation. METHODS: We developed a portable perfusion apparatus for use in donor heart preservation. Contractile, metabolic, and vasomotor functions were monitored simultaneously in an isolated swine heart. The metabolic state was monitored by myocardial tissue pH. Vasomotor function was assessed in isolated coronary ring chambers. Hearts were randomized into 3 groups: group I (n = 5), cardioplegic arrest, 12-hour storage at 4 degrees C with modified Belzer solution, and 2-hour sanguineous reperfusion in the working state; group II (n = 6), 12-hour continuous perfusion in the beating working state, 30 minutes of arrest (to simulate re-implantation time), and 2 hours of reperfusion, as above; group III (n = 7), coronary ring control hearts. RESULTS: At 2 hours of reperfusion, left ventricular developed pressure in group II was higher than in group I (mean +/- standard deviation: 90 +/- 6 mm Hg, 53 +/- 15 mm Hg, P = .005). Significantly less myocardial edema was observed in group II than in group I (73% +/- 4%, 80% +/- 1% water content, P = .01). Significantly less myocardial acidosis was noted in group II than in group I during preservation (pH 7.3 +/- 0.01, 6.1 +/- 0.03, P < .001) and reperfusion (pH 7.3 +/- 0.008, 6.8 +/- 0.05, P < .001). Coronary endothelial vasomotor function was better preserved in group II than in group I as evidenced by dose-response relaxation of coronary rings to 10(-8) mol/L bradykinin (37%, 55% delta baseline, P = .01). CONCLUSION: This new method extends the current preservation limit and avoids time-dependent ischemic injury, thereby allowing for distant procurement of donor organs.
Assuntos
Transplante de Coração/fisiologia , Contração Miocárdica/fisiologia , Preservação de Órgãos , Animais , Circulação Coronária/fisiologia , Metabolismo Energético/fisiologia , Parada Cardíaca Induzida , Masculino , Traumatismo por Reperfusão Miocárdica/fisiopatologia , Preservação de Órgãos/instrumentação , Perfusão , Sobrevivência de Tecidos/fisiologiaRESUMO
BACKGROUND: Platelet dysfunction and increased fibrinolysis are the most important etiologic factors in the hemostatic defect observed following the institution of cardiopulmonary bypass. This study examined the effects of heparin per se, administered before the institution of cardiopulmonary bypass, on platelet function and fibrinolysis. METHODS: Sampling was performed in 55 patients undergoing cardiac operations before and 5 minutes after the routine administration of heparin, before the institution of cardiopulmonary bypass. RESULTS: Heparin administration resulted in a significant prolongation of the bleeding time (from 6.3 +/- 2.1 to 12.6 +/- 4.9 minutes; p < 0.00001), a significant reduction in the level of shed blood thromboxane B2 (from 1,152 +/- 669 to 538 +/- 187 pg/0.1 mL; p = 0.00002), and an increase in the plasma levels of plasmin (from 11.8 +/- 9.7 to 125.4 +/- 34.8 U/L; p < 0.0001) and D-dimer (from 571.3 +/- 297.1 to 698.5 +/- 358.6 micrograms/mL; p = 0.05). There were no significant differences before and after heparin administration in the plasma levels of fibrinogen, plasminogen, tissue plasminogen activator, antiplasmin, antithrombin III, and von Willebrand factor. CONCLUSIONS: Heparin, independent of cardiopulmonary bypass, causes both platelet dysfunction and increased fibrinolysis. The use of an alternative anticoagulant or a lower dose of heparin in conjunction with heparin-coated surfaces might improve the hemostatic balance during open heart operations.
Assuntos
Anticoagulantes/efeitos adversos , Plaquetas/efeitos dos fármacos , Ponte Cardiopulmonar , Fibrinólise/efeitos dos fármacos , Heparina/efeitos adversos , Idoso , Testes de Coagulação Sanguínea , Feminino , Hemostasia/efeitos dos fármacos , Humanos , Masculino , Pessoa de Meia-Idade , Cuidados Pré-OperatóriosRESUMO
The ability to differentiate intraoperatively between myocardial stunning, which is reversible, and irreversible myocardial infarction has major implications because it provides a rational approach to the use or withholding of ventricular assist devices in patients with severe postcardiotomy ventricular dysfunction. Two illustrative cases are presented.
Assuntos
Procedimentos Cirúrgicos Cardíacos , Ecocardiografia Transesofagiana , Infarto do Miocárdio/diagnóstico , Miocárdio Atordoado/diagnóstico , Idoso , Evolução Fatal , Humanos , Concentração de Íons de Hidrogênio , Período Intraoperatório , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/diagnóstico por imagem , Infarto do Miocárdio/fisiopatologia , Miocárdio Atordoado/diagnóstico por imagem , Miocárdio Atordoado/fisiopatologia , Miocárdio/química , Função Ventricular EsquerdaRESUMO
In order to study the metabolic consequences of myocardial stunning, repeated coronary occlusions were performed in dogs. The production of CO2, adenosine triphosphate (ATP), phosphocreatine (PCr), and inorganic phosphate (Pi) by myocardial cells was assessed, along with extracellular and intracellular pH. Our results indicate that regional coronary artery occlusion reduces the ability of the myocardium to produce H+ and CO2 and to replenish ATP post ischemia. These alterations, then, represent the hallmark of metabolic viability during periods of ischemic insult. Decreases in PCr and Pi were completely eliminated during reperfusion and, therefore, are ot reflective of myocardial stunning. When normothermic cardiopulmonary bypass (CPB) is instituted and the coronary artery is occluded three times with reperfusion between each occlusion, alterations in myocardial H+ and high energy phosphates are identical to those observed using only repetitive coronary occlusion. Systemic hypothermia during CPB does not protect against myocardial stunning; however, it is anticipated that interventions that prevent the reduction in H+ and ATP levels may overcome the effects of myocardial stunning that occur during cardiac surgery.
Assuntos
Cardiomiopatias/etiologia , Cardiomiopatias/metabolismo , Ponte Cardiopulmonar/efeitos adversos , Contração Miocárdica/fisiologia , Miocárdio/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Dióxido de Carbono/metabolismo , Cardiomiopatias/fisiopatologia , Cães , Hipotermia Induzida/efeitos adversos , Reperfusão Miocárdica/efeitos adversos , Fosfatos/metabolismo , Fosfocreatina/metabolismoRESUMO
Left ventricular isovolumic stress development and metabolic parameters were studied in 18-24-month-old spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto (WKY) rat controls using the isolated, isovolumic (balloon in left ventricle) buffer-perfused rat heart preparation. After WKY rats and all SHRs were compared, SHRs were divided into two groups: those animals with (SHR-F) and without (SHR-NF) evidence of heart failure. Hearts were perfused at 100 mm Hg using a constant pressure system at a temperature of 37 degrees C. In the baseline state, peak systolic pressure was greatest in the SHR-NF group and lowest in the SHR-F group. Peak midwall stress was greatest in the WKY group and, again, lowest in the SHR-F group. Oxygen consumption was lowest in the SHR-F group. When the oxygen cost of stress development was estimated by normalizing myocardial oxygen consumption by peak developed midwall stress, values were lowest in the WKY, greater in the SHR-NF, and greatest in the SHR-F group. Lactate production did not occur in the baseline state in any of the groups. Functional and metabolic responses to graded hypoxia, induced by changing the gas mixture of the perfusate from 95% to 50%, 25%, and 0% oxygen at perfusion pressures of 100 and 130 mm Hg, were studied. Increasing perfusion pressure generally resulted in small increases in peak systolic pressure and myocardial oxygen consumption but did not substantially reverse the contractile or metabolic deficit present in the SHR-F group.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Cardiomegalia/metabolismo , Insuficiência Cardíaca/metabolismo , Hipertensão/metabolismo , Consumo de Oxigênio , Animais , Cardiomegalia/fisiopatologia , Insuficiência Cardíaca/fisiopatologia , Hemodinâmica , Hipertensão/fisiopatologia , Hipóxia/metabolismo , Masculino , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Estresse MecânicoRESUMO
Rat dorsal skin flaps predictably underwent full-thickness necrosis by 48 hours when hematoma had been placed beneath the flap. Microangiographic studies demonstrated failure of filling of the distal vasculature of these flaps. This was in marked contrast to the complete reestablishment of circulation in control flaps, overlying equal volumes of serum. The circulation of failing flaps overlying hematoma was restored with isoxsuprine given parenterally 1 hour preoperatively and every 4 hours for 24 hours postoperatively. This pharmacologic regimen gave consistent reestablishment of flap perfusion and flap survival. The toxic component of hematoma acts on the circulation to a skin flap. This may be at the same level of the vasculature as the vasodilating action of isoxsuprine.
Assuntos
Angiografia , Sobrevivência de Enxerto/efeitos dos fármacos , Isoxsuprina/farmacologia , Retalhos Cirúrgicos , Animais , Hematoma/diagnóstico por imagem , Hematoma/patologia , Masculino , Microrradiografia , Necrose , Ratos , Pele/irrigação sanguínea , Pele/patologia , Transplante Isogênico , Cicatrização/efeitos dos fármacosRESUMO
The present study was designed to test the hypothesis that povidone-iodine would inhibit the recovery of tensile strength in a healing wound. Clean, incised dorsal wounds in rats were soaked in 1% povidone-iodine solution for 15 minutes; control wounds were soaked in lactated Ringer's solution before closure. Tensile strength measurements and histologic studies were conducted at 1, 2, and 6 weeks. No statistical differences in the rate of gain of tensile strength or histological appearance were noted between control and experimental wounds. It is concluded that brief irrigation of clean incised wounds with povidone-iodine solution does not affect factors important in the recovery of tensile strength during would healing, such as fibroplasia and collagen cross-linking.