RESUMO
Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time ( approximately 2 min) to perform. These results suggest that the rapid stain may be more cost-effective and efficient for use in clinical laboratories.
Assuntos
Fluorescência , Mycobacterium/isolamento & purificação , Coloração e Rotulagem/métodos , Tuberculose/diagnóstico , Benzofenoneídio/farmacologia , Corantes Fluorescentes/farmacologia , Humanos , Sensibilidade e Especificidade , Fatores de TempoRESUMO
Mycobacterium sp. recovery and time to detection were compared in the MGIT 960 and BacT/ALERT MB automated broth culture systems. The MGIT 960 demonstrated shorter time to detection (13.5 versus 25.2 days) and greater sensitivity (100% versus 66.6%) for recovery of the Mycobacterium tuberculosis complex than the BacT/ALERT MB system.
Assuntos
Técnicas Bacteriológicas/métodos , Mycobacterium tuberculosis/isolamento & purificação , Tuberculose/diagnóstico , Automação , Humanos , Sensibilidade e Especificidade , Fatores de Tempo , Tuberculose/microbiologiaRESUMO
Two methods were compared for decreasing bacterial contamination in the BacT/Alert mycobacterial culture detection system. Two concentrations, 0.5 ml (standard amount) and 1.0 ml, of mycobacterial antibiotic supplement were evaluated. Contamination rates were 14% and 6% for the standard and the doubled concentrations, respectively. This difference was statistically significant (P < 0.005).
