RESUMO
Although a considerable amount of work has been carried out in the last ten years in developing methods for the separation of steroids by HPLC, it is still not widespread for the reasons discussed above. There is however no doubt that further developments in HPLC technology, in increasing sensitivity and/or specificity of detection systems, perhaps with microbore columns, may lead to an increase in the use of this powerful analytical procedure as an additional separation method to improve specificity of assay. Solution of the problem of simple interfacing of HPLC systems with mass spectrometers (discussed in another chapter by Games) should further increase the application of HPLC. HPLC is of particular value in providing a means of separating unstable compounds prior to assay by relatively nonspecific quantitation methods. Most steroids do not fall into this category, but the steroid vitamin D and its metabolites do and HPLC has proved in this area to be invaluable (see chapter by Jones & DeLuca). There are a multiplicity of different HPLC systems for the separation of steroids, varying in column type (and manufacturer), solvent composition and method of elution, temperature of elution, etc., and only a few attempts have been made to rationalise these data. It would therefore seem that a fruitful area of future study would be the investigation of computerised systems for the selection and optimisation of HPLC systems for particular steroid separations.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Esteroides/análise , Corticosteroides/análise , Corticosteroides/biossíntese , Androgênios/análise , Androgênios/biossíntese , Fenômenos Químicos , Química , Colesterol/análise , Colesterol/metabolismo , Estrogênios/análise , Estrogênios/biossíntese , Humanos , Progesterona/análise , Progesterona/biossíntese , Receptores de Esteroides/análise , Esteroides/metabolismoRESUMO
After a brief discussion of the merits and limitations of high-pressure liquid chromatography (HPLC) relative to other chromatographic methods, special problems in the application to steroids are discussed. Publications on HPLC of steroids are then discussed under the headings of individual classes, arranged generally in the order of increasing polarity.
Assuntos
Esteroides/análise , Córtex Suprarrenal/análise , Alcaloides/análise , Androstanos/análise , Animais , Ácidos e Sais Biliares/análise , Glicosídeos Cardíacos/análise , Cromatografia Líquida de Alta Pressão/métodos , Estrogênios/análise , Pregnanos/análise , Sapogeninas/análise , Esteróis/análise , Vitamina D/análiseRESUMO
High-pressure liquid chromatography was used to separate the following steroidal alkaloids: tomatidine, solanidine, solasodine, rubijervine, veratramine and jervine. The method was used to prepare crystalline solanidine from a crude mixture of aglycones obtained from Solanum chacoense, and to separate radioactive solanidine from extracts of potato plants fed with [4-C]cholesterol.
Assuntos
Alcaloides/análise , Cromatografia Líquida de Alta Pressão , Esteroides/análise , Métodos , Piperidinas/análise , Alcaloides de Solanáceas , Espirostanos/análise , Tomatina/análogos & derivados , Alcaloides de Veratrum/análiseAssuntos
Hormônios , Plantas/metabolismo , Esteroides , Androstanos/metabolismo , Fenômenos Químicos , Química , Colecalciferol/metabolismo , Colesterol/análogos & derivados , Colesterol/metabolismo , Ecdisona/metabolismo , Ecdisterona/metabolismo , Ergocalciferóis/metabolismo , Estradiol/metabolismo , Estrona/metabolismo , Fungos/metabolismo , Hormônios/metabolismo , Hormônios de Inseto/metabolismo , Fotossíntese , Fitosteróis/metabolismo , Sitosteroides/metabolismo , Esteroides/antagonistas & inibidores , Esteroides/metabolismo , Testosterona/metabolismoAssuntos
Plantas/metabolismo , Esteroides/metabolismo , Alcaloides/metabolismo , Androstenodiona/metabolismo , Glicosídeos Cardíacos/metabolismo , Digitoxina/metabolismo , Estradiol/metabolismo , Estrona/metabolismo , Hormônios Esteroides Gonadais/metabolismo , Pregnanodionas/metabolismo , Pregnanos/metabolismo , Pregnenolona/metabolismo , Progesterona/metabolismo , Saponinas/metabolismo , Especificidade da Espécie , Estrofantidina/metabolismo , Testosterona/metabolismoRESUMO
A single dose of mevalonic acid-2-(14)C was administered simultaneously to 6 Haplopappus heterophyllus plants. They were harvested at intervals ranging from 3 days to 6 months. Four groups of biosynthetically related sterols were found to be radioactive in each plant, and the changes in radioactivity with time were studied. The most striking finding was a radioactive phenolic material present only in the 6-month plant, which had flowered.
RESUMO
Pharbitis nil seedlings rapidly metabolized (-)-kaurene-17-(14)C administered to the cotyledons. Less than 20% of the radioactivity was recovered by extraction of the cotyledons on the following day. Of this the major metabolite was an unidentified acidic material which did not correspond chromatographically to any of the known gibberellins.Significant differences were observed between the radioactivity found in the buds of a group of seedlings subjected to photoperiodic floral induction and that in a noninduced group. In both cases almost all of this radioactivity was in the neutral fraction, but thin-layer chromatography of these fractions revealed that the induced group contained 2 or more labelled components which were either absent or present in lower concentrations in the noninduced group.
