How to objectively determine the color of beer?

Beer color is an important sensory attribute, the first one that the consumer observes. There are two standard methods accepted for determining the color of these products, one related to the European Brewery Convention (EBC) and the other is the Standard Reference Method (SRM). Both methods are based on absorbance, but in case of the more and more popular fruit beers these methods give false result since these products appear in varied colors and have different spectra than regular beers. In this study 39 different types of beers were investigated, including fruit beers and beer based mixed drinks to compare their color in CIE 1976 L*a*b* color space, absorption-based colors and transmission spectra. ΔE*ab values of products with less than 5% EBC difference ranged from 4.5 to 17.4. There were magnitude differences in the transmission spectra of these products, fruit beers showed different tendencies due to the added fruit or fruit juice. The highest ΔE*ab value belonged to two traditional Weissbiers. Absorption-based methods are not able in many cases to differentiate between products which have nearly the same EBC or SRM color but visually are different. A multi-wavelength method would be reasonable to be developed for more objective and accurate beer color determination.

Study of antioxidant activity during the malting and brewing process.

In this study the evolution of antioxidant activity was investigated during malting of different barley cultivars, and during the production of different types of beers on laboratory scale and in pilot brewery. Samples were taken at technologically important points of productions. Malts were produced from 3 spring and 3 winter barley cultivars. Two types of beers were brewed under laboratory conditions, and two in a pilot brewery. For the determination of antioxidant activity five commonly used assays were applied such as ABTS Radical Scavenging Activity, Cupric Reducing Antioxidant Capacity, DPPH Radical Scavenging Activity, Ferric Reducing Antioxidant Power and Total Polyphenol Content. Prior to malting it was observed that there are orders of magnitude differences between the antioxidant activities of the barley varieties. During malting, the biggest increase was noticed during steeping. Spring and winter cultivars showed similar trends during steeping and germination, but kilning had different effect on antioxidant activity of the varieties. The antioxidant activity of malts was always higher than the corresponding barleys. During the brewing process antioxidants were released to the highest extent during the early stages of mashing. Adequate sparging and hop boiling could further improve the antioxidant potential of the wort. Furthermore, differences between the equipment used for wort separation and hop boiling under laboratory conditions and in the pilot brewery had effect on antioxidant activity. In the course of malting and brewing by selecting the appropriate raw materials and technological parameters, the conditions for the release and retention of antioxidants can be optimized.

A comparative assessment of antioxidant properties, total phenolic content of einkorn, wheat, barley and their malts.

Two einkorn wheat, one barley, three optional winter cultivation wheat and five winter cultivation wheat samples harvested in Hungary in 2011, and their malts were evaluated for their DPPH radical and ABTS radical cation scavenging activity, ferric reduction capacity (FRAP) and total phenolic content (TPC). All einkorn and barley samples exhibited significant antioxidant activities determined by DPPH and ABTS radical scavenging activities. The einkorn samples show higher polyphenol content than the other wheat samples. In all cases the barley sample had the highest antioxidant potential and polyphenol content. The einkorn malts had high DPPH and ABTS radical cation scavenging activities, but the phenolic content was lower against wheat samples. There was significant difference between the antioxidant potential of optional and winter cultivation wheat samples except on ABTS scavenging activities. Einkorn wheat is potentially a new raw material to produce organic beer that might have beneficial effects with its increased antioxidant potential.