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1.
Science ; 377(6602): eabo0924, 2022 07 08.
Artigo em Inglês | MEDLINE | ID: mdl-35737810

RESUMO

The human cerebral cortex houses 1000 times more neurons than that of the cerebral cortex of a mouse, but the possible differences in synaptic circuits between these species are still poorly understood. We used three-dimensional electron microscopy of mouse, macaque, and human cortical samples to study their cell type composition and synaptic circuit architecture. The 2.5-fold increase in interneurons in humans compared with mice was compensated by a change in axonal connection probabilities and therefore did not yield a commensurate increase in inhibitory-versus-excitatory synaptic input balance on human pyramidal cells. Rather, increased inhibition created an expanded interneuron-to-interneuron network, driven by an expansion of interneuron-targeting interneuron types and an increase in their synaptic selectivity for interneuron innervation. These constitute key neuronal network alterations in the human cortex.


Assuntos
Córtex Cerebral , Conectoma , Animais , Córtex Cerebral/ultraestrutura , Humanos , Interneurônios/ultraestrutura , Macaca , Camundongos , Células Piramidais/ultraestrutura
2.
Science ; 371(6528)2021 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-33273061

RESUMO

Brain circuits in the neocortex develop from diverse types of neurons that migrate and form synapses. Here we quantify the circuit patterns of synaptogenesis for inhibitory interneurons in the developing mouse somatosensory cortex. We studied synaptic innervation of cell bodies, apical dendrites, and axon initial segments using three-dimensional electron microscopy focusing on the first 4 weeks postnatally (postnatal days P5 to P28). We found that innervation of apical dendrites occurs early and specifically: Target preference is already almost at adult levels at P5. Axons innervating cell bodies, on the other hand, gradually acquire specificity from P5 to P9, likely via synaptic overabundance followed by antispecific synapse removal. Chandelier axons show first target preference by P14 but develop full target specificity almost completely by P28, which is consistent with a combination of axon outgrowth and off-target synapse removal. This connectomic developmental profile reveals how inhibitory axons in the mouse cortex establish brain circuitry during development.


Assuntos
Conectoma , Neurônios GABAérgicos/fisiologia , Interneurônios/fisiologia , Rede Nervosa/crescimento & desenvolvimento , Córtex Somatossensorial/crescimento & desenvolvimento , Sinapses/fisiologia , Animais , Axônios/ultraestrutura , Conjuntos de Dados como Assunto , Dendritos/ultraestrutura , Neurônios GABAérgicos/ultraestrutura , Imageamento Tridimensional/métodos , Interneurônios/ultraestrutura , Camundongos , Microscopia Eletrônica/métodos , Rede Nervosa/ultraestrutura , Córtex Somatossensorial/ultraestrutura , Sinapses/ultraestrutura
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