Prevalence of cervicitis in dairy cows and its effect on reproduction.

The objective of this study was to determine whether cervicitis in dairy cows is an independent disease or occurs concomitantly with inflammation of the uterus, and to clarify possible effects of cervicitis on reproductive performance. Dairy cows (n = 416) from 33 dairy farms were examined by rectal palpation and vaginoscopy between 42 and 50 days postpartum. Inclusion criteria for this study were absence of abnormal vaginal discharge and abnormalities of the uterus (fluctuation) at rectal palpation. Cervicitis was diagnosed when the second cervical fold was swollen and prolapsed with (C2) or without (C1) reddening. Cytobrush samples from the uterus (n = 370) and the cervix (n = 402) were collected, and the percentage of neutrophils in the uterus (PMNU) and the cervix as indicators of inflammation (threshold: ≥5%) was determined. In addition, endometrial biopsies for histology were collected, 300 of which were suitable for evaluation. Cervicitis (C1/C2) was diagnosed in 253 of 416 (60.8%) of cows. Of these, the prolapsed cervical mucosa was hyperemic (C2) in 29.1% of cases. Of 370 available uterine cytology samples, 221 cows had a clinical cervicitis; however, 170 (76.9%) had PMNU less than 5%. Of 300 uterine histologic examinations, 82 (27.3%) did not reveal any abnormalities; the remaining cows either had uterine inflammation and/or degenerative uterine changes such as endometriosis and angiosclerosis. Furthermore, of 300 biopsied animals, 184 revealed a cervicitis (C1/C2); however, 30.4% of these animals had no histopathologic uterine findings. For further analysis, only animals either without histopathologic findings and normal uterine cytology or with solely endometritis (defined as PMNU ≥ 5% and/or positive histopathology of the uterine tissue) were evaluated (n = 157). Of these, 95 cows had cervicitis. Unexpectedly, 63 of 95 (66.3%) cows had cervicitis without endometritis. With regard to reproductive performance, days to first service were not affected by cervicitis. Number of days open in animals with cervicitis but without endometritis tended to be lower than in cows with cervicitis plus endometritis (P = 0.092). Also, number of days open relative to percentage of neutrophils greater than 5% was lower when the cervical compared to the uterine mucosa was affected (P < 0.05). Total conception and pregnancy rates of animals 200 days into lactation decreased significantly in cows with severe cervical inflammation (C2). In conclusion, the results of this study suggested that cervicitis occurs independent of endometritis, and a higher degree of cervicitis is associated with poorer reproductive performance.

[Synchronization of ovulation (OVSYNCH) in high producing dairy cattle herds. Part II: Milk yield, diseases and cost-benefit-analysis]. / Ovulationssynchronisation (OVSYNCH) in hochleistenden Milchviehherden: II. Milchleistung, Erkrankungen und Kosten-Nutzen-Analyse.

A method for synchronization of ovulation (OVSYNCH) was evaluated for its practical use in seven North German dairy cattle herds. The original procedure of PURSLEY et al. (1995) was applied, i. e. cows were treated with Gonadotropin Releasing Hormone (GnRH) at a random stage of the estrous cycle, followed by an injection of a prostaglandin F2alpha (PGF2alpha)-analog seven days later and another injection of GnRH two days after PGF2alpha. Cows were inseminated 24 h after the last injection by artificial insemination. Control animals were assigned by corresponding lactation number and month of lactation. Cows from the OVSYNCH group returned to the conventional reproductive management after the OVSYNCH treatment, which was the same management as that for the control group. The parameters of fertility and correlations between body condition score (BCS) as well as concentrations of serum progesterone and the success of OVSYNCH were presented in the first report. For the OVSYNCH group, the 100-day-duction during the time period from calving until a pregnancy was established differed markedly from healthy cows in a variety of parameters. Healthy cows had a greater FSC (65.22%) than sick cows (35.90%) (P < 0.05). Furthermore, mean days open were less in healthy cows than in sick cows (85.48 +/- 30.56 days vs 110.85 +/- 46.40 days) (P < 0.05). Cows showing a disease had a higher number of lactation (4.00 +/- 2.26 vs. 2.80 +/- 1.35; P < 0.01) and a decreased BCS (2.72 +/- 0.23 vs. 2.91 +/- 0.23; P < 0.05). Sick and healthy cows from the OVSYNCH group did not differ with regard to 100-day-milk-yield (milk kg, relative and absolute milk fat and protein content). The cost-benefit-analysis revealed higher total cost, and cost per pregnancy (16,408.07 euros and 278.10 euros, respectively) in the control group than in the OVSYNCH group (11,015.69 euros and 196.71 euros, respectively).

[Synchronization of ovulation (OVSYNCH) in high-producing dairy cattle herds. I. Fertility parameters, body condition score and plama progesterone contration]. / Ovulationssynchronisation (OVSYNCH) in hochleistenden Milchviehherden: I. Fruchtbarkeitsparameter, Body Condition Score und Plasmaprogesteronkonzentration.

A method for synchronization of ovulation (OVSYNCH) was evaluated for its practical use in seven North German dairy cattle herds. The original procedure of PURSLEY et al. (1995) was applied, i.e. cows were injected Gonadotropin Releasing Hormone (GnRH) at a random stage of the estrous cycle, followed by an injection of a prostaglandin F2 alpha (PGF2 alpha)-analogon seven days later and another injection of GnRH two days after PGF2 alpha. Cows were inseminated 24 h after the last injection by artificial insemination. Control animals were assigned by corresponding lactation number and stage of lactation (month of partus). Cows from the OVSYNCH group returned to the conventional reproductive management after the OVSYNCH treatment, which was the same management as that for the control group. The average milk yield in 1997 and 1998 was 8128.6 +/- 637.0 kg and 8688.3 +/- 665.0 kg per year, respectively. First service conception rate (FSC) in the OVSYNCH group (n = 187) (39.77%) was lower than in the controls (n = 175) (54.34%; P < 0.01). In 1997, FSC for the OVSYNCH group did not differ from controls (48.94% vs. 55.45%; P > 0.05), whereas in 1998, FSC for the OVSYNCH group (29.27%) was significantly lower than in the control group (29.27% vs. 52.78%) and the OVSYNCH group in 1997 (each time P < 0.01). First service conception rate of first lactation cows in the OVSYNCH group was significantly lower than in the control group (37.84% vs. 71.05%; P < 0.01), whereas no difference was found for the other lactation groups. The mean interval calving to first service was shortened in the OVSYNCH group by about 14 d as compared with the controls (P < 0.01). However, mean days open only differed by 2.92 d (P > 0.05). Proportion of cows in each class of lactation number (1., 2. and > 2. lactation number) with days open < or = 115 d in the OVSYNCH group were similar to controls (P > 0.05). There was a great influence of body condition on the success of OVSYNCH. Cows with a body condition score (BCS) of 3.00 had a significant better FSC than cows with a BCS < 3.00 (P < 0.05). First service conception rate was lower for cows with a BCS > 3.25 and < 2.75 (cows in good and poor condition taken together) than for cows with a BCS of 3.00 (P < 0.05). A positive effect of OVSYNCH was found in acyclic cows as determined by progesterone concentration in blood plasma. Nine out of eleven cows reacted with the resumption of ovarian activity. Two cows became pregnant after first insemination.

[Examination of the appropriateness of anamnestic and clinical parameters for the prediction of the course of pregnancy under field conditions]. / Zur Uberprüfung geeigneter anamnestischer und klinischer Parameter für eine Abfohlprognose unter Praxisbedingungen.

In this paper, fertility relevant parameters which are routinely examined in practice were evaluated on the basis of their correlation to one another and their influence on the foaling rates and incidence of early embryonic death in 175 brood mares of the Hanover breeding stock. The aim was to evaluate the suitability of these parameters for inclusion in a scheme for increasing the precision of predicting the subsequent course of pregnancy, as compared to such prognoses based exclusively on the histological evaluation of the endometrium. After establishing the breeding history and completing a clinical examination, two swab specimens were obtained for bacteriological culture and cytologic smears and one biopsy specimen was taken from the endometrium for histological examination. The most statistically significant effect on the foaling rate (analysed by logistic regression with two variables) have biopsy grade (p = 0.0087) and mare age (p = 0.0259). A mare between two and nine years of age with a biopsy grade of 1 has a 71% probability of carrying a pregnancy to term. This probability decreases to 43% for a mare 15 years and older with the same biopsy grade. Mare age had a statistically significant effect on biopsy grade (p = 0.005) and on the frequency and degree of endometrosis (p = 0.001). The mare group was significantly correlated (p = 0.012) with the foaling rate (maiden mares 70.6%, foaling mares 60%, problem mares 36.1%). There is a difference of 24% between the foaling rates of foaling and problem mares, although the age and biopsy grade distributions are almost identical in the two groups. The number of barren years had a statistically significant effect (p = 0.0235) on the foaling rate. Mares that bore a foal in the previous breeding period had a foaling rate of 50.7% in the year of examination; mares that had been barren for one year hat a subsequent foaling rate of 40.6%. Mares that had been barren for two years or more had a foaling rate of only 22.7%. The overall impression resulting from the clinical examination was significantly correlated with the foaling rate (p = 0.018%). Mares without abnormal findings had a foaling rate of 54.2%, mares with poor vulval conformation a rate of 46.7%. Mares with a history of pregnancy loss in the current breeding period had a foaling rate of 25% and mares with clinical evidence of endometritis had one of 22.2%.

[Consequences of a surgical correction of an insufficient closure of the vulva on genital flora and conception rate in mares]. / Auswirkungen der chirurgischen Korrektur eines mangelhaften Schamschlusses auf die Genitalflora und die Konzeptionsrate bei Stuten.

In this thesis the influence of pneumo-vagina on the microbiological colonization of the genital tract and their manifestation in cytological smears was examined. For mares with poor vulval conformation a comparison of the bacterial growth before and after plastic surgery of the vulva and vestibulum was carried out, as well as the registration of conception rates after operation and insemination. The biggest reduction of the bacterial content in the reproductive tract was found between vestibulum and cranial section of the vagina. The increased number of contaminant bacteria in the cranial section of the vagina during abnormal vulval conformation could be reduced significantly by the plastic surgery of the vulva and vestibulum (p < 0.05). Assuming that an 18-20 days pregnant uterus is free from a colonization with virulent bacteria, a high significant reduction of the increased number of contaminant bacteria in the uterus during vulval incompetence was found after surgical correction (p < 0.01). In mares with poor conformation of the vulva, which had not been operated, the contamination of the reproductive tract had increased at the time of checkup in comparison to the first swab specimens, taken 18-20 days before. The share of mares with a negative bacteriological and positive cytological result was in case of vulval incompetence twice as large as if they had a proper vulval conformation. A decrease of these latent infections was found after plastic surgery of the vulva and vestibulum. From the operated mares a total of 72.9% became pregnant.

[The genital microflora in the stallion--microbiological study of presecretion samples from 1972 to 1991]. / Zur genitalen Keimflora beim Hengst--Mikrobiologische Untersuchung von Vorsekretproben in der Zeit von 1972 bis 1991.

From 1972 to 1991, presecretion on an average of 183 stallions per year had been examined. Facultatively pathogenic germs were ascertained in 13.5% of the samples. However, the portion showed considerable annual fluctuation, as did the percentage of the different kinds of germs. In most of the cases, only minimal growth had been observed in culture. Except for the total percentage, these results are contradictory to those we found during the examination of cervical swabs of mares. In order to ascertain the importance of the respective results and to render possible a correct interpretation, the constancy of the facultatively pathogenic germs was investigated additionally. It was distinctly dependent on the quantity and--to a lesser extent--on the kind of the germs as well. According to the present results, minimal growth in culture throughout only indicates a temporary colonization of the genitals. These results--except for the indication of CEMO--may be neglected, unless otherwise indicated by clinical findings or by anamnesis.