New technique for rapid purification, entrapment, and recovery of enterotoxin A from a liquid chamber by polyacrylamide gel electrophoresis.

Electrophoreis of enterotoxin into a middle liquid section of a polyacrylamide gel column enhances recovery for serological assay.

Heat inactivation of enterotoxin A from Staphylococcus aureus in veronal buffer.

Serological tests were used to determine the slope of the thermal inactivation curve of crude enterotoxin A in Veronal buffer (pH 7.2), and the resulting z value was 27.8 C. (50 F). Serological assays also showed that the heat inactivation at each time-temperature depended on the original concentration of enterotoxin A. The usefulness of the Oudin tube serological test for determining end points of inactivation of naturally produced enterotoxin A (not concentrated) is discussed. We concluded that this test cannot be used to determine end points of heat inactivation for enterotoxin A in the minute quantities naturally produced in foods.