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Arch Virol ; 165(2): 463-470, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31863266

RESUMO

The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher amongst porcine sequences, where four G genotypes (G3, G4, G5, G11), two P genotypes (P[6], P[13]) and one I5 genotype were detected. Human RVA strains were represented by two G genotypes (G1, G3), two I genotypes (I1, I2), and one P genotype (P[8]). Genetic analysis did not show a relationship between Slovakian porcine and human RVA strains, but phylogenetic grouping of some Slovakian porcine sequences with Hungarian human sequences in both G and P genotypes was observed.


Assuntos
Variação Genética , Infecções por Rotavirus/veterinária , Infecções por Rotavirus/virologia , Rotavirus/classificação , Rotavirus/genética , Doenças dos Suínos/virologia , Animais , Antígenos Virais/genética , Proteínas do Capsídeo/genética , Fezes/virologia , Genótipo , Humanos , Reto/virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rotavirus/isolamento & purificação , Análise de Sequência de DNA , Eslováquia , Sus scrofa , Suínos
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