Effects of supplementing pregnant heifers with methionine or melatonin on the anatomy and other characteristics of their lateral hind claws.

Three groups of four primiparous Holstein-Friesian heifers were fed throughout pregnancy either a control diet or that diet supplemented with either 5 to 6 g per day of rumen-protected intestinally available methionine or 25 mg melatonin. They were euthanased three days after calving. The dietary supplements had no effect on the impression hardness or the concentrations of cysteine and methionine in samples of claw horn collected from a range of sites, or on the areas of erosion in the sole and heel. Significant differences were recorded for the hardness of the horn in the order wall >sole >heel. These differences were associated with higher concentrations of cysteine and lower concentrations of methionine in samples of horn from the dorsal wall than in samples from the prebulbar region of the sole. There were no significant differences attributable to the dietary supplements in the soft tissue anatomy of the solear dermis and epidermis.

Effects of time of year and reproductive state on the proliferation and keratinisation of bovine hoof cells.

Cell proliferation and protein synthesis (keratinisation) were measured in vitro in hoof biopsy samples taken from two groups of seven heifers, the first calving in the winter and the second in the summer. Both parameters were significantly higher in summer than in winter irrespective of the heifers' reproductive state. The mean (se) measure of the rate of protein synthesis was 199 (27) dpm/microg DNA/hour in summer and 4 (1) dpm/microg DNA/hour in winter, and the equivalent values for cell proliferation (measured as DNA synthesis) were 375 (56) dpm/microg DNA/hour and 17 (4) dpm/microg DNA/hour. Changes around parturition depended on the time of the year. In the winter-calving heifers, the rates of proliferation and keratinisation increased significantly after calving from 22.3 (7.2) to 70.4 (16.6) and from 2.1 (0.7) to 12.4 (2.8) dpm/microg DNA/hour, respectively. In the summer-calving heifers, proliferation decreased from 388.2 (91.0) to 66.7 (9.6) dpm/microg DNA/hour but the rate of keratinisation did not change. Lesion scores and locomotion scores deteriorated after parturition, especially in the winter-calving group. The hooves were harder in summer than winter but their hardness was not affected by the heifers' reproductive state.