Serum s100b protein levels as a neuroinflammatory biomarker of acutely relapsed paranoid schizophrenia patients.

OBJECTIVE: The s100b inflammatory protein is involved in schizophrenia pathophysiology. We aim at studying the evolution of the s100b serum levels in acutely relapsed paranoid schizophrenia patients at three different time points (admission, discharge and 3 months after hospital discharge 3MAHD). METHODS: Twenty-three paranoid schizophrenia inpatients meeting DSM-IV criteria participated in the research. Twenty-three healthy subjects matched by age, gender and season acted as the control group. Psychopathology was measured with the Positive and Negative Syndrome Scale (PANSS). Serum s100b levels were determined at 12:00 and 24:00 h with an enzyme-linked immunoassay kit. RESULTS: Patients had significant higher serum s100b levels at admission and discharge (12:00 h) than the group of healthy subjects. At admission and discharge, s100b serum levels at 24 h had decreased compared to the 24:00 h s100b levels of the healthy subjects. At 3MAHD patients and healthy subjects had similar levels of serum s100b protein. Positive and negative PANSS scores decreased significantly between admission and discharge. Positive and negative PANSS scores decreased between discharge and 3MAHD, but these changes had no statistical significance. CONCLUSIONS: Our study confirms that the acute inflammatory response produced in acutely relapsed patients is reversed after 3 month of hospital discharge. The variations of serum s100b concentrations when the patients suffer from an acute relapse may be a useful predictor of disease evolution.

Descriptive study of diving injuries in the Canary Islands from 2008 to 2017.

INTRODUCTION: This research reports the epidemiology of diving injuries managed in the Hyperbaric Medicine Unit of the Canary Islands University Hospital. METHODS: Data were extracted from the clinical records of all divers injured and admitted to the unit for treatment of dysbaric diving injuries between 2008 and 2017, inclusive. RESULTS: One-hundred and thirty diving injuries were recorded. Most (71%) occurred in men and 43% were foreigners. Eighteen per cent either had no diving certification or that information was not recorded in the clinical chart. Only a third of the 40% of divers who had some form of on-site first aid treatment received oxygen and oral rehydration. Type 1 decompression sickness (DCS) was diagnosed in 56 divers (43%) and Type 2 in 67 (52%), whilst seven were treated for omitted decompression. At discharge, 122 (94%) were asymptomatic, whilst 5% experienced some residual sensory or other changes. One diver who presented late remained quadriparetic and one, admitted in a state of coma, died. Only 76% of the injured divers had specific diving accident insurance and, of those, 58% were foreign divers. CONCLUSIONS: Over half of the injured divers did not receive any on-site first aid. The majority (94%) of treated injured divers were discharged without sequelae. Based on these data, several public health recommendations for the Canary Islands are made.

Day/Night and Summer/Winter Changes in Serum Total Antioxidant Capacity.

BACKGROUND: Seasonal and circadian changes are two factors described to affect blood levels of some biological molecules. The Total Antioxidant Capacity (TAC) is one global measure of the antioxidant capacity of a system. There is no agreement about the existence of day/night changes in TAC levels as well as there is no information about seasonal changes in TAC levels. OBJECTIVE: The aims of this research are studying if there are summer/winter changes in TAC concentrations or if TAC concentrations have day/night changes. METHOD: Ninety-eight healthy subjects took part in the summer study of whom 64 participated in the winter one. Blood was sampled at 09:00, 12:00 and 00:00 h. TAC was measured by the ABTS radical cation technique. Results are expressed in mmol/L of trolox equivalents. RESULTS: The subjects had significantly higher TAC levels in summer than winter at the three-time point studied. Summer 09:00 TAC concentration was significantly higher than the 12:00 and 00:00 h concentrations (1.34±0.26 vs 0.83±0.19, 0.75±0.18). Summer TAC 12:00 h concentrations were significantly higher than the 00:00 h concentrations (0.83±0.19 vs. 0.75±0.18). Winter 09:00 TAC concentrations were significantly higher than the 12:00 and 00:00 h concentrations (1.24±0.16 vs. 0.73±0.10, 0.67±0.13). There were no significant differences between the 12:00 and 00:00 h TAC concentrations. CONCLUSION: Strong methodological biases may be made if the seasonal and circadian changes in serum TAC concentration are not taken into account when researching in this area.

Chronotype as modulator of morning serum melatonin levels.

BACKGROUND: The search for biological markers of individual characteristics has produced scanty results. Melatonin (MLT), the main hormonal product of the pineal gland, has been used as a biological marker of neuroticism, introversion-extroversion and morningness-eveningness. Morningness-eveningness indicates preferences associated with morning or evening activities. The goal of this research is to study if serum MLT levels are related to morningness-eveningness preference. METHODS: Twenty-three morning type and twenty-one evening type healthy volunteers took part in the study. Morningness-eveningness was evaluated with the Composite Scale of Morningness. Blood was drawn at 09:00, 12:00 and 00:00 h. MLT levels were measured with an ELISA. RESULTS: At 09:00 h evening type subjects had significantly higher serum MLT levels than morning type subjects (8.4±3.6 pg./ml. vs. 4.6±3.2 pg./ml., p<0.02). CONCLUSIONS: Morning serum MLT may be used as a biological peripheral marker of morningness-eveningness preference. Our results emphasise the convenience of expanding MLT studies until 09:00 h when differences between morning type and evening type subjects may still be found.

El cronotipo como modulador de los niveles séricos diurnos de melatonina / Chronotype as modulator of morning serum melatonin levels

Introducción. La búsqueda de marcadores biológicos que se relacionen con características específicas de las personas no ha producido grandes resultados. Los niveles sanguíneos de la melatonina (MLT), principal producto hormonal de la glándula pineal, han sido utilizados como marcador biológico del neuroticismo, la introversión-extroversión y la matutinidad vespertinidad. El concepto de matutinidad hace referencia a la preferencia de las personas para realizar actividades por las mañanas, mientras que la vespertinidad hace referencia a la preferencia para realizar actividades por la noche. El objetivo de este trabajo consiste en estudiar si los niveles séricos de MLT se relacionan con la matutinidad o vespertinidad. Metodología. La muestra está compuesta por 44 voluntarios sanos, de los cuales 23 son del tipo matutino y 21 del tipo vespertino. La matutinidad-vespertinidad fue valorada con la Escala Compuesta de Matutinidad. Se analizaron 3 muestras de sangre, extraídas a las 09:00, 12:00 y 00:00 h. Los niveles de MLT fueron determinados mediante un ELISA. Resultados. A las 09:00 h, los sujetos vespertinos tenían niveles de MLT significativamente más altos que los sujetos matutinos (8,4±3,6 pg/ml vs 4,6±3,2 pg/ml, p<0,02).Conclusiones. Los niveles séricos de MLT a las 09:00 h. pueden ser usados como un marcador biológico periférico de vespertinidad-matutinidad. Nuestros resultados enfatizan la conveniencia de alargar los estudios de MLT al menos hasta las09:00 h, cuando aun se pueden encontrar diferencias en los niveles séricos de MLT entre los tipos matutinos y vespertinos (AU)

Background. The search for biological markers of individual characteristics has produced scanty results. Melatonin (MLT), the main hormonal product of the pinealgl and, has been used as a biological marker of neuroticism, introversion-extroversion and morningness-eveningness. Morningness-eveningness indicates preferences associated with morning or evening activities. The goal of this research is to study if serum MLT levels are related to morningness eveningness preference. Methods. Twenty-three morning type and twenty-one evening type healthy volunteers took part in the study. Morningness-eveningness was evaluated with the Composite Scale of Morningness. Blood was drawn at 09:00, 12:00 and 00:00 h. MLT levels were measured with an ELISA. Results. At 09:00 h evening type subjects had significantly higher serum MLT levels than morning type subjects (8.4±3.6 pg./ml. vs. 4.6±3.2 pg./ml., p<0.02). Conclusions. Morning serum MLT may be used as a biological peripheral marker of morningness-eveningness preference. Our results emphasise the convenience of expanding MLT studies until 09:00 h when differences between morning type and evening type subjects may still be found (AU)

Summer/winter changes in serum S100B protein concentration as a source of research variance.

BACKGROUND: S100B is a calcium binding protein that can be measured in cerebral and extra cerebral biological tissues and fluids. Circadian and seasonal variations have been described in several biological molecules such as melatonin, cortisol and testosterone. Healthy subjects do not have a circadian rhythm of S100B. There is no information on seasonal variations of S100B levels. The aim of this research is to study whether healthy subjects present summer/winter changes in serum S100B protein concentrations. METHODS: Ninety-eight subjects were studied in summer, of those, 64 participated in the winter evaluation. Blood was drawn by venipuncture at 09:00 h, 12:00 h and 00:00 h in summer and winter. Serum was separated from blood by centrifugation and stored at -70° until analysis. Serum S100B concentrations were measured by ELISA. RESULTS: Serum S100B concentrations were significantly higher in summer than winter (09:00 h: 43.4 ± 24.6 ng/ml vs. 29.3 ± 22.7 ng/ml, p < 0.001; 12:00 h: 42.8 ± 25.0 ng/ml vs. 23.0 ± 22.1 ng/ml, p < 0.001; 00:00 h: 44.5 ± 23.2 ng/ml vs. 28.5 ± 24.6 ng/ml, p < 0.001). Age, gender, body mass index and time points when blood was extracted did not affect serum S100B concentrations neither in summer nor in winter. CONCLUSIONS: Our results point to the fact that there is an important difference in serum S100B concentrations between summer and winter. It is strongly advisable to consider this summer/winter difference in serum S100B concentrations when researching into this area.