Antitumor Effects of 10058-F4 and Curcumin in Combination Therapy for Pancreatic Cancer In Vitro and In Vivo.

Background: Pancreatic cancer (PC) stands out as one of the most lethal cancers. Due to late diagnosis, only a fraction of patients can be resected. Although it still has significant adverse effects and poor results, the treatment is connected with better overall survival than the prior treatment. Thus, new alternative therapy for advanced PC is needed. Materials/Methods. The impact of 10058-F4 and curcumin combination therapy on apoptosis and cell growth in SW1990 pancreatic cancer cells were determined in vitro using the CCK-8 assay and flow cytometry of Annexin V-FITC/PI, and the in vivo antitumor effect was determined utilizing SW1990-bearing pancreatic tumor mouse models induced by subcutaneous implantation. Results: At concentrations of (10 mol/L+2 mol/L), 10058-F4+curcumin obtained the highest rate of SW1990 cell death, and they had a beneficial effect on SW1990 pancreatic tumor-bearing animals. Furthermore, c-Myc, Akt phosphorylation, and the expression of apoptosis-related molecular were reduced, and the combination therapy modified the expression of apoptosis-related molecular. Conclusions: In vitro and in vivo, the combination of 10058-F4 plus curcumin has antipancreatic cancer actions that are substantially effective.

Effect of ultrasound-guided intercostobrachial nerve blockade in patients with post-mastectomy pain syndrome / 临床麻醉学杂志

Objective To investigate and evaluate the feasibility and analgesic effect of ultrasound guided intercostobrachial nerve (ICBN) blockade in patients with persistent pain after radical mastectomy with ICBN preservation.Methods In a total of 53 following-up female cases,aged 18-55 years,ASA Ⅰ or Ⅱ,receiving radical mastectomy with ICBN preservation during January,2014 to February,2016,19 patients complained persistent pain in the lateral chest,axilla and/or upper arm more than 3 months after the surgery were enrolled.ICBN blockade was performed using 0.5% ropivacine 10 mL injected in the potential space between the superior of pectoralis minor muscle and the inferior of serratus anterior muscle in the second intercostal space guided by ultrasound.Visual analogue scale (VAS) was applied to assess the pain intensity at rest,on movement,and with 100 kPa pressure before blockage and 30 min thereafter,and then the pain intensity (SPI) was calculated.All nerve blockade-related adverse events including local anesthetic-related allergy,central nervous system and cardiovascular toxicity were recorded.Seven-day follow-ups were required in all patients to assess the pain intensity.Results All 19 cases completed ICBN blockade guided by ultrasound,of which 17 cases completed the seven-day follow-ups.The mean difference in SPI was-9.19 VAS points (95%CI-11.24--7.14,P<0.01).Compared with each mean VAS and SPI before block,all that after block at day 1-6 were significantly decreased (P<0.01 or P<0.05).There were 14(82.4%),13(76.5%),7(41.2%) and 3(17.6%)cases that SPI was decreased over 5 points after block at day 1-4.No nerve blockade-related adverse event was observed.Conclusion Ultrasound guided ICBN blockade can relieve persistent post-mastectomy pain safely and effectively.

The ocular optic fiber used in the endoscopic sinus surgery of dacryocystorhinostomy in the treatment of chronic dacryocystitis and recurrent dacryocystitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore a method for locating the area of lacrimal sac in dacryocystorhinostomy under endoscopy.@*METHOD@#Sixty-eight patients were performed dacryocystorhinostomy under endoscopy. Take light spot of ocular optic fiber as the lacrimal sac projection to the lateral wall of the nasal cavity position.@*RESULT@#With the guiding of ocular optic fiber, lacrimal sac can be located accurately. The operating time of dacryocystorhinostomy under endoscopy was shortened significantly, and the operation procedure was simplified. All patients were followed up for 2 years, only 2 recurrent cases were found. The success rate reach to 97.06% (66/68).@*CONCLUSION@#Ocular optical fiber used in locating the lacrimal sac in dacryocystorhinostomy under endoscopy is simple and feasible, and can be widely used.

A clinical study of dacryocystorhinostomy per nasal endoscope guided by endo-ophthalmic optical fiber / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To get a method of dacryocyst allocation for dacryocystorhinostomy per nasal endoscope.@*METHOD@#The dacryocyst of 7 chronic dacryocystitis cases(8 eyes) were located through nasal cavity guided by endo-ophthalmic optical fiber.@*RESULT@#All the 7 patients got successful operations, the bleeding was 10-20 ml during the operation , postoperative recovery was good, no infection occurred. Five patients received operations twice, all of them were detained anaesthetic tubes for 3 to 6 months, dacryocyst rinsing were conducted once per month after extubation. No obstructions and complications occurred after one year follow up.@*CONCLUSION@#Endo ophthalmic optical fiber can be used to locate dacryocyst in dacryocystorhinostomy per nasal endoscope.

The Influence of Furosemide on the Currents of the Delayed Rectification Potassium Channels and Sodium Channels of Mice's Spiral Ganglion Neurons / 听力学及言语疾病杂志

Objective To study the influence of furosemide on the currents of the delayed rectification potassium channel and sodium channel of mice's spiral ganglion neurons.Methods Postnatal mice(P1～P6) spiral ganglion neurons were obtained by mechanical dissociation and enzymolysis.Delayed rectification potassium channels' currents and sodium channels' currents were recorded with whole-cell patch clamp techniques.Observed was the influence of furosemide on potassium channels and sodium channels.Results When furosemide was added around the spiral ganglion neurons,the delayed rectification potassium currents were inhibited to around +200～+300 pA,and sodium currents were inhibited to about 30% of peak current.Furosemide was washed away after working steadily for 1 minute,5 and 10 minutes.The delayed rectification potassium currents could recover to 98%,64%,and 25% of the peak currents before and sodium currents could recover to 96%,76% and 54% of the peak currents accordingly.Conclusion The currents of delayed rectification potassium channels and sodium channels could be inhibited by furosemide to different degrees.The longer furosemide was used,the greater damage could occur in the ion channels.

Clinical Study on Wrist-Ankle Acupuncture for Treatment of 30 Cases of Peripheral Neuritis Complicated by Diabetes / 中医杂志

Objective:To study on mechanisms of wrist-ankle acupuncture for prevention and treatment of peripheral neuritis complicated by diabetes.Methods:Ninety cases of peripheral neuritis complicated by diabetes were treated respectively by wrist-ankle acupuncture and body acupuncture,with western medicine routine treatment used as control group,30 cases in each group.The therapeutic effects and laboratory results were compared.Results:Therapeutic effects in wrist-ankle acupuncture group and body-acupuncture group were significantly superior to that in control group,with no significant difference between wrist-ankle acupuncture and body-acupuncture group.Conclusion:Wrist-ankle acupuncture can improve blood glueose and metabolism blood lipids,decrease blood viscosity and recover function of peripheral nerve cells and it has a definite effect on diabetic peripheral neuritis.