RESUMO
AIMS: To establish the role of cardiolipin (CL) of the membrane in response to the presence of tetradecyltrimethylammonium in Pseudomonas putida A (ATCC 12633). METHODS AND RESULTS: Two ORFs of Ps. putida A (ATCC 12633), which in Ps. putida KT2440 encode the putative CL synthase genes cls and cls2, were cloned, sequenced and mutated. Only the double mutant lacking cls and cls2 showed a reduction of the CL content, 83% lower than the amount produced by the wild-type. Accompanying this change was a 40% decrease in the content of unsaturated fatty acid. Consequently, the membrane of the mutant was more rigid than the one of the parental strain, as observed using fluorescence polarization techniques. The mutant strain showed reduced viability in the presence of tetradecyltrimethylammonium. The incorporation of exogenous CL into its membrane relieved sensitivity to the cationic detergent. CONCLUSIONS: Pseudomonas Putida cells with low levels of CL die in the presence of tetradecyltrimethylammonium, because they cannot counter the fluidizing effect of the cationic surfactant. SIGNIFICANCE AND IMPACT OF THE STUDY: The modification in the membrane phospholipids composition allows knowing the adaptation strategy of Ps. putida when these bacteria are exposed to cationic surfactant.
Assuntos
Antibacterianos/farmacologia , Pseudomonas putida/efeitos dos fármacos , Tensoativos/farmacologia , Compostos de Trimetil Amônio/farmacologia , Cardiolipinas/análise , Cardiolipinas/metabolismo , Clonagem Molecular , Polarização de Fluorescência , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Fases de Leitura Aberta , Fosfolipídeos/metabolismo , Pseudomonas putida/química , Pseudomonas putida/genética , Pseudomonas putida/metabolismo , Transferases (Outros Grupos de Fosfato Substituídos)/genéticaRESUMO
Twenty-six patients, aged 21 to 63, hospitalized for depression, and 26 control individuals were asked to make a "funny" and a "sad" drawing, which were scored on 22 variables describing their formal aspects and contents. Results show that drawings of individuals with depression differ from those of control participants on a wide range of variables, sometimes very significantly. Prediction of participants' groups based on eight variables obtained by discriminant analysis was correct in 98% of cases. Results support the usefulness of asking for the two kinds of drawings and suggest that depression is better detected through formal aspects of drawings than through their contents.
Assuntos
Arteterapia , Transtorno Depressivo/psicologia , Emoções , Adulto , Transtorno Depressivo/diagnóstico , Diagnóstico Diferencial , Expressão Facial , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
A patient with fully expressed, sporadic ectrodactyly, ectodermal dysplasia, and cleft lip and/or palate (EEC) syndrome with renal involvement is presented; descriptions of four similar patients were found in the literature. Based on these patients, we suggest that the renal involvement is a form of dysplasia secondary to fetal urinary tract obstruction. We emphasize the importance of searching for a urinary tract anomaly in patients with this uncommon syndrome.
Assuntos
Anormalidades Múltiplas , Fissura Palatina , Displasia Ectodérmica , Deformidades Congênitas do Pé , Deformidades Congênitas da Mão , Sistema Urinário/anormalidades , Humanos , Recém-Nascido , Rim/patologia , Masculino , SíndromeRESUMO
An enameler with dysphagia was found to have extrinsic compression of the esophagus by enlarged mediastinal lymph nodes. Scalene lymph node biopsy revealed silicosis, and tissue cultures grew Mycobacterium intracellulare. We believe our patient is the first reported to have dysphagia due to silicotic adenopathy complicated by an atypical mycobacteriosis.
