Evaluation of nickel-zinc interactions by means of bioassays with amphibian embryos.

The nickel hazard was evaluated by means of a 7-day toxicity test with Bufo arenarum embryos. The LC(50) values for this metal from 24 to 168 h diminished from about 26 to 1.8 mg Ni(2+)/L, respectively, but from 96 h onward, the LC(50) varied very slightly. Although a noticeable difference among the LC(50) and LC(10) or LC(90) was observed at 24 h of exposure, these parameters tended to a similar value at 168 h of exposure while the confidence intervals of LC(50) overlapped all other confidence interval values. These results, plotted as toxicity profile curves, are useful for determining time and concentration thresholds for Ni. Nickel-zinc interactions on B. arenarum embryos were evaluated by means of simultaneous treatments with both cations (Ni: 5-35 mg Ni(2+)/L; Zn: 0.5-130 mg Zn(2+)/L). As a general pattern, low Zn concentrations (0.5 mg Zn(2+)/L) did not have a clear-cut effect on Ni toxicity, higher Zn concentrations (2-20 mg Zn(2+)/L) enhanced Ni toxicity, and concentrations of 30 mg Zn(2+)/L and higher had a beneficial effect in most cases. The metal interaction studies provide a scientific basis for the establishment of water quality criteria for wildlife protection purposes.

Ecotoxicity in the Reconquista River, province of Buenos Aires, Argentina: a preliminary study.

The Reconquista River in Argentina is considered a "supercritical" river basin due to environmental degradation. Within its valley of 1.547 km2, there are more than 3 million inhabitants and 12,000 industries. Using early-life-stage toxicity tests with Bufo arenarum embryos (the most sensitive of three native species), we determined the water quality at six sampling stations of the river valley and expressed the results as acute and chronic toxicity units. Along most of the river, the toxicity was higher than the allowable level of whole industrial effluent toxicity recommended by U.S. EPA. In a tributary stream, Arroyo Moron, the water was about 10 times more toxic than the criteria maximum concentration (CMC) recommended by U.S. EPA for industrial effluents. Similar degradation of the water quality was found taking as a reference value an upstream sampling station. In all places where the water quality was worse than the CMC, no macroorganisms were found, and in one of these places, a large number of dead fishes was observed. Our study points out that amphibian early-life-stage toxicity tests could be appropriate for assessing water contamination and water quality, which is essential for diagnosis, protection of environmental services, monitoring, and restoration purposes.