COVID-19 and Emotional Variables in a Sample of Chileans.

INTRODUCTION: During the Coronavirus (COVID-19) pandemic, a set of daily stressors are being experienced, all this affects people's mental health, leading them to have a set of emotional disturbances. Little is known about how people's age can influence their emotional well-being in the face of prolonged stress generate by the pandemic. OBJECTIVE: To clarify the presence of emotional aspects such as emotional expressiveness and the frequency of positive and negative affections in people with different age in times of crisis. METHODS: The final sample included 297 Chileans between 22 and 68 years old (M = 38.51, SD = 13.85), recruited through an online survey with the appropriate written informed consent. The study was carried out when the pandemic was spreading in Chile. RESULTS: The findings revealed age differences in emotional expressivity and the type of affections experienced. The expression of emotions was more affected by negative affections, the age and the gender of the people. While the avoidance of this emotional expression, by age and affections both positive and negative. Age was a significant predictor of emotional expressiveness. DISCUSSION: Findings suggests that the associations between both variables, varied according to the age group of the people. Furthermore, this finding proposes that although older people are facing the persistent and serious threat of COVID-19, they show better emotional functioning. Which would help to better understand the interaction of both positive and negative life experiences in times of crisis.

[Assessment of the concentrations of carbonylated proteins and carbonyl reductase enzyme in mexican women with breast cancer: A pilot study]. / Determinación de proteínas carboniladas y enzima carbonil reductasa en mujeres mexicanas con cáncer de mama: estudio piloto.

Oxidative stress could promote the development of cancer and implicate carbonylated proteins in the carcinogenic process. The goal of this study was to assess the concentrations of carbonylated proteins and carbonyl reductase enzyme in women with breast cancer and determine whether these markers were possible indicators of tissue damage caused by the disease. A total of 120 healthy women and 123 women with a diagnosis of breast cancer were included. The concentration of carbonylated proteins in plasma and the concentration of carbonyl reductase enzyme in leukocytes were determined using the ELISA assay. There was a 3.76-fold increase in the amount of carbonylated proteins in the plasma from the patient group compared with healthy control group (5±3.27 vs. 1.33±2.31 nmol carbonyls/mg protein; p<0.05). Additionally, a 60% increase in the carbonyl reductase enzyme was observed in the patient group compared with the healthy control group (3.27±0.124 vs. 2.04±0.11 ng/mg protein; p<0.05). A positive correlation (r=0.95; p<0.001) was found between both measurements. These results suggest the presence of tissue damage produced by cancer; therefore, these parameters could be used to indicate tissue damage in cancer patients.

In vitro effect of sodium fluoride on malondialdehyde concentration and on superoxide dismutase, catalase, and glutathione peroxidase in human erythrocytes.

The aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress. For this purpose, human erythrocytes were incubated with NaF (0, 7, 28, 56, and 100 µg/mL) or NaF (100 µg/mL) + vitamin E (1, 2.5, 5 and 10 µg/mL). The malondialdehyde (MDA) concentration on the surface of the erythrocytes was determined, as were the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GlPx). Our results demonstrated that erythrocytes incubated with increasing NaF concentrations had an increased MDA concentration, along with decreased activity of antioxidant enzymes. The presence of vitamin E partially reversed the toxic effects of NaF on erythrocytes. These findings suggest that NaF induces oxidative stress in erythrocytes in vitro, and this stress is partially reversed by the presence of vitamin E.

[May-Thurner syndrome as a cause of neurovascular compression of the pudendal nerve]. / Síndrome de May-Thurner como causa de compresión neurovascular del nervio pudendo.

May-Thurner syndrome is a clinical condition that results from narrowing of the left common iliac vein lumen due to pressure from the right common iliac artery as it crosses anterior to it. We describe an atypical case of May-Thurner syndrome in a 23-year-old woman that presented only continuous pain in pudendal zone without vascular symptoms. The Doppler ultrasound, nuclear magnetic resonance and others complementary analyses show the presence of a pelvic venous congestion syndrome and we hypothesized that this condition produced a neuropathic compression of the pudendal nerve in Alcock's canal. Patient was treated with the technique of pudendal nerve blockade by trans-gluteal via. An important reduction in pain of pudendal zone was showed.

[Detection of human papillomavirus types 16 and 18 in semen samples from patients in an assisted reproduction program]. / Deteccién del virus del papiloma humano tipos 16 y 18 en muestras de semen de pacientes de un programa de reproducción asistida.

BACKGROUND: The infection with human papillomavirus (HPV) represents a serious health problem in the world. This is because are associated with cervical cancer development in when HPV of high-risk type 16 and 18 are involved. A genital infection by these virus types are normally asymptomatic in the male and an infection to sexual partner is possible. OBJECTIVE: The objective of the present is the detection of HPV-16 and HPV-18 in semen samples from patients included in an assisted reproduction protocol. MATERIAL AND METHOD: Semen samples were obtained from 149 patients that are included in an assisted reproduction protocol in our institution. Semen was examined according with WHO criteria and detection of HPV-16 and HPV-18 was realized with real time PCR protocol with commercial kits. Also, conventional histology techniques were used to assess spermatozoo morphology and leukocyte count. RESULTS: 149 semen samples were analyzed from patients with an age average 37.27 (22-56 age). The 56.18% present oligozoospermic and take into account all patients, 59.73% was positive for HPV-16 (29.56%), HPV-18 (16.11%) or both (14.09%). The infection with HPV-16 was more frequent in both oligozoospermic and normozoospermic patients. In this latter, a minus abnormal spermatic cells and leukocytes were found in regard to oligozoospermic patients. CONCLUSIONS: There is an important percentage off human asymptomatic male subjects that present in your semen a positive result for high risk papillomavirus. This is very important for the sexual partner because this represent a problem for public health that most be in attention.