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J Bioenerg Biomembr ; 43(6): 757-64, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22108703

RESUMO

Permeability transition was examined in heart mitochondria isolated from neonate rats. We found that these mitochondria were more susceptible to Ca(2+)-induced membrane leakiness than mitochondria from adult rats. In K(+) containing medium, at 25 °C, mitochondria were unable to accumulate Ca(2+). Conversely, in Na(+) containing medium, mitochondria accumulated effectively Ca(2+). At 15 °C mitochondria accumulated Ca(2+) regardless of the presence of K(+). Kinetics of Ca(2+) accumulation showed a similar Vmax as that of adult mitochondria. Lipid milieu of inner membrane contained more unsaturated fatty acids than adult mitochondria. Aconitase inhibition and high thiobarbituric acid-reactive substances (TBARS) indicate that oxidative stress caused mitochondrial damage. In addition, proteomics analysis showed that there is a considerable diminution of succinate dehydrogenase C and subunit 4 of cytochrome oxidase in neonate mitochondria. Our proposal is that dysfunction of the respiratory chain makes neonate mitochondria more susceptible to damage by oxidative stress.


Assuntos
Cálcio/farmacologia , Mitocôndrias Cardíacas/metabolismo , Membranas Mitocondriais/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Animais , Transporte de Elétrons/efeitos dos fármacos , Permeabilidade/efeitos dos fármacos , Potássio/metabolismo , Ratos
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