Novel noninvasive embryo selection algorithm combining time-lapse morphokinetics and oxidative status of the spent embryo culture medium.

OBJECTIVE: To develop a noninvasive embryo selection algorithm consisting of time-lapse morphokinetics and the oxidative status of the spent embryo culture medium determined using the Thermochemiluminescence (TCL) Analyzer. DESIGN: Retrospective cohort. SETTING: Not applicable. PATIENT(S): From women participating in the oocyte donation program, data from 505 samples of spent embryo culture media samples from 292 intracytoplasmic sperm injection cycles. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Morphokinetic parameters assessed during incubation in the time-lapse system Embryoscope. Oxidative parameters (H1sm, H2sm, and H3sm) from the spent culture medium on day 5 of incubation measured using the TCL assay; and a combined assessment algorithm, including morphology, morphokinetics, and the embryo's culture medium oxidative status, developed as a tool for embryo selection, based on implantation success and confirmed ongoing pregnancy. RESULT(S): The levels of the oxidative parameters H1sm, H2sm, and H3sm on day 5 of incubation were statistically significantly higher in transferred and vitrified embryos compared with discarded embryos and in successfully implanted embryos compared with those that did not result in pregnancy. The assessment algorithm resulted in a hierarchical classification with six embryo quality categories (A to F), associated with implantation rates of between 76.5% and 29.2%. CONCLUSION(S): An assessment algorithm combining morphology, morphokinetics and the embryo's culture medium oxidative status may help to improve current embryo selection methods criteria and in vitro fertilization success.

Tratamiento Homeopático de la Verruga Plantar. Caso Clínico Tratado en una Farmacia Comunitaria / Homeopathic Wart Treatment Plant. Clinical Case Treated in a Community Pharmacy

Las verrugas plantares son un tipo de infección causadas por el virus del papiloma humano (VPH). En muchos de los casos son muy dolorosas y difíciles de tratar; aunque generalmente desaparecen con tratamiento en unos meses, en otros casos persisten durante años. Se presenta el caso de un varón de 13 años que acudió a la oficina de farmacia tras el fracaso de un prolongado tratamiento convencional para una verruga plantar en el talón. Existen antecedentes documentados y experiencias favorables en el tratamiento homeopático de las verrugas plantares y de su utilización en farmacia. Se le pautaron Thuja occidentalis 15CH (10 gránulos semanalmente), Dulcamara 9CH (5-0-5), Antimonium crudum 15CH (5-0-5) y Nitricum acidum 15CH (5-0-5) durante 1 mes. Se observó una mejoría clínica importante al cabo de sólo 1 mes exclusivamente con tratamiento homeopático, que puede suponer una posibilidad de tratamiento eficaz para las verrugas plantares, incluso tras el fracaso de otras intervenciones convencionales.(AU)

Plantar warts are a type of infection caused by the human papillomavirus. In many cases they are very painful and difficult to treat, they usually disappear in a few months with the treatment but in several cases they will persist for years. The case of a 13 years old male is presented, who came to the pharmacy after a long and unsuccessful conventional treatment of a plantar wart on the heel of the left foot. The plantar warts have been successfully treated by homeopathic medicines in the bibliography and according to our own experience. The chosen medicines were Thuja occidentalis 15CH (10 granules weekly), Dulcamara 9CH 5 (5-0-5), Antimonium crudum 15CH (5-0-5) and Nitricum acidum 15CH (5-0-5). An important improvement was observed after 1 month of treatment. The homeopathic treatment can be an effective treatment option for plantar warts, including those where other conventional interventions have not succeeded. (AU)