RESUMO
5-Hydroxy-L-tryptophan (5HW) has been biosynthetically incorporated in many proteins to facilitate their characterization using fluorescence spectroscopy. An attractive feature of this tryptophan analogue is its absorbance at 310-320 nm, allowing its specific excitation in a Trp background. The red-shift in absorbance upon introduction of a hydroxyl group at the 5-position of Trp or indole was found to be due to a lowering of the (1)Lb transition energy. It was therefore believed that 5HW only features (1)Lb emission. Recently, calculations for 5-hydroxyindole (5HI) in water revealed (1)La is the emitting state, and the same was predicted for 5HW incorporated in proteins. To clarify which state emits in 5HI and 5HW, we present here excitation anisotropy spectra of these probes and of four proteins labeled with 5HW at a surface exposed position. Our data clearly show (1)Lb is the emitting state of 5HI, 5HW, and 5HW in three of the proteins investigated. For one protein mixed emission was observed, and the decay kinetics were found strongly dependent on the emission wavelength. This work provides the first experimental evidence that (1)La can be the emitting state for this Trp analogue incorporated in a protein.
Assuntos
5-Hidroxitriptofano/química , Indóis/química , Proteínas/química , Polarização de Fluorescência , Mutação , Espectrometria de Fluorescência , Triptofano/química , Triptofano/genéticaRESUMO
The mannitol transporter EII(mtl) from Escherichia coli is responsible for the uptake of mannitol over the inner membrane and its concomitant phosphorylation. EII(mtl) is functional as a dimer and its membrane-embedded C domain, IIC(mtl), harbors one high affinity mannitol binding site. To characterize this domain in more detail the microenvironments of thirteen residue positions were explored by 5-fluorotryptophan (5-FTrp) fluorescence spectroscopy. Because of the simpler photophysics of 5-FTrp compared to Trp, one can distinguish between the two 5-FTrp probes present in dimeric IIC(mtl). At many labeled positions, the microenvironment of the 5-FTrps in the two protomers differs. Spectroscopic properties of three mutants labeled at positions 198, 251, and 260 show that two conserved motifs (Asn194-His195 and Gly254-Ile255-His256-Glu257) are located in well-structured parts of IIC(mtl). Mannitol binding has a large impact on the structure around position 198, while only minor changes are induced at positions 251 and 260. Phosphorylation of the cytoplasmic B domain of EII(mtl) is sensed by 5-FTrp at positions 30, 42, 251 and 260. We conclude that many parts of the IIC(mtl) structure are involved in the sugar translocation. The structure of EII(mtl), as investigated in this work, differs from the recently solved structure of a IIC protein transporting diacetylchitobiose, ChbC, and also belonging to the glucose superfamily of EII sugar transporters. In EII(mtl), the sugar binding site is more close to the periplasmic face and the structure of the 2 protomers in the dimer is different, while both protomers in the ChbC dimer are essentially the same.
Assuntos
Proteínas de Escherichia coli/química , Escherichia coli/química , Manitol/química , Proteínas de Transporte de Monossacarídeos/química , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/química , Triptofano/análogos & derivados , Motivos de Aminoácidos , Transporte Biológico Ativo/fisiologia , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Manitol/metabolismo , Proteínas de Transporte de Monossacarídeos/genética , Proteínas de Transporte de Monossacarídeos/metabolismo , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/genética , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/metabolismo , Fosforilação , Ligação Proteica , Estrutura Quaternária de Proteína , Estrutura Terciária de Proteína , Espectrometria de Fluorescência/métodos , Triptofano/químicaRESUMO
The mannitol transporter from Escherichia coli, EII(mtl), belongs to a class of membrane proteins coupling the transport of substrates with their chemical modification. EII(mtl) is functional as a homodimer, and it harbors one high affinity mannitol-binding site in the membrane-embedded C domain (IIC(mtl)). To localize this binding site, 19 single Trp-containing mutants of EII(mtl) were biosynthetically labeled with 5-fluorotryptophan (5-FTrp) and mixed with azi-mannitol, a substrate analog acting as a Förster resonance energy transfer (FRET) acceptor. Typically, for mutants showing FRET, only one 5-FTrp was involved, whereas the 5-FTrp from the other monomer was too distant. This proves that the mannitol-binding site is asymmetrically positioned in dimeric IIC(mtl). Combined with the available two-dimensional projection maps of IIC(mtl), it is concluded that a second resting binding site is present in this transporter. Active transport of mannitol only takes place when EII(mtl) becomes phosphorylated at Cys(384) in the cytoplasmic B domain. Stably phosphorylated EII(mtl) mutants were constructed, and FRET experiments showed that the position of mannitol in IIC(mtl) remains the same. We conclude that during the transport cycle, the phosphorylated B domain has to move to the mannitol-binding site, located in the middle of the membrane, to phosphorylate mannitol.
Assuntos
Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Proteínas de Transporte de Monossacarídeos/química , Proteínas de Transporte de Monossacarídeos/metabolismo , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/química , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/metabolismo , Sítios de Ligação/genética , Sítios de Ligação/fisiologia , Proteínas de Escherichia coli/genética , Transferência Ressonante de Energia de Fluorescência , Manitol/análogos & derivados , Manitol/metabolismo , Modelos Biológicos , Proteínas de Transporte de Monossacarídeos/genética , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/genética , Fosforilação , Ligação Proteica/genética , Ligação Proteica/fisiologia , Multimerização Proteica/genética , Multimerização Proteica/fisiologia , Espectrometria de FluorescênciaRESUMO
In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol translocation process. To spectroscopically distinguish between the Trp positions in each subunit of dimeric EII(mtl), 5-FTrp was biosynthetically incorporated because of its much simpler photophysics compared to those of Trp. The steady-state and time-resolved fluorescence methodologies used point out that all four positions are in structured environments, both in the absence and in the presence of a saturating concentration of mannitol. The fluorescence decay of all 5-FTrp-containing mutants was highly homogeneous, suggesting similar microenvironments for both probes per dimer. However, Stern-Volmer quenching experiments using potassium iodide indicate different solvent accessibilities for the two probes at positions 97 and 133. A 5 A two-dimensional (2D) projection map of the membrane-embedded IIC(mtl) dimer showing 2-fold symmetry is available. The results of this work are in better agreement with a 7 A projection map from a single 2D crystal on which no symmetry was imposed.
Assuntos
Proteínas de Escherichia coli/química , Escherichia coli/enzimologia , Proteínas de Transporte de Monossacarídeos/química , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/química , Triptofano/análogos & derivados , Triptofano/química , Sequência de Aminoácidos , Citoplasma/química , Citoplasma/metabolismo , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Manitol/química , Manitol/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Proteínas de Transporte de Monossacarídeos/genética , Proteínas de Transporte de Monossacarídeos/metabolismo , Mutação , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/genética , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/metabolismo , Conformação Proteica , Espectrometria de Fluorescência , Relação Estrutura-Atividade , Triptofano/metabolismoAssuntos
Proteínas de Bactérias/química , Triptofano/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Metionina/genética , Metionina/metabolismo , Mutação/genética , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/metabolismo , Rhodospirillum rubrum/genética , Rhodospirillum rubrum/metabolismo , Espectrometria de Fluorescência , Triptofano/genética , Triptofano/metabolismoRESUMO
This work reports an explanation for the unusual monoexponential fluorescence decay of 5-fluorotryptophan (5FTrp) in single-Trp mutant proteins [Broos, J.; Maddalena, F.; Hesp, B. H. J. Am. Chem. Soc. 2004, 126, 22-23] and substantially clarifies the origin of the ubiquitous nonexponential fluorescence decay of tryptophan in proteins. Our results strongly suggest that the extent of nonexponential fluorescence decay is governed primarily by the efficiency of electron transfer (ET) quenching by a nearby amide group in the peptide bond. Fluoro substitution increases the ionization potential (IP) of indole, thereby suppressing the ET rate, leading to a longer average lifetime and therefore a more homogeneous decay. We report experimental IPs for a number of substituted indoles including 5-fluoroindole, 5-fluoro-3-methylindole, and 6-fluoroindole, along with accurate ab initio calculations of the IPs for these and 20 related molecules. The results predict the IP of 5-fluorotryptophan to be 0.19 eV higher than that of tryptophan. 5-Fluoro substitution does not measurably alter the excitation-induced change in permanent dipole moment nor does it change the fluorescent state from 1La to 1Lb. In combination with electronic structure information this argues that the increased IP and the decreased excitation energy of the 1La state, together 0.3 eV, are solely responsible for the strong reduction of electron transfer quenching. 6-Fluoro substitution is predicted to increase the IP by a mere 0.09 eV. In agreement with our conclusions, the fluorescence decay curves of 6-fluorotryptophan-containing proteins are well fit using only two decay times compared to three required for Trp.
Assuntos
Indóis/química , Proteínas/química , Triptofano/análogos & derivados , Triptofano/química , Eletroquímica , Polarização de Fluorescência , Espectrometria de Fluorescência , Espectrofotometria UltravioletaRESUMO
Tryptophan, when in a protein, typically shows multiexponential fluorescence decay kinetics. Complex kinetics prevents a straightforward interpretation of time-resolved fluorescence protein data, particularly in anisotropy studies or if the effect of a dynamic quencher or a resonance energy transfer (RET) acceptor is investigated. Here, time-resolved fluorescence data are presented of an isosteric tryptophan analogue, 5-fluorotryptophan, which when biosynthetically incorporated in proteins shows monoexponential decay kinetics. Data are presented indicating that the presence of a fluoro atom at the 5-position suppresses the electron transfer rate from the excited indole moiety to the peptide bond. This process has been related to the multiexponential fluorescence decay of tryptophan in proteins. The monoexponential decay of 5-fluorotryptophan makes it possible to measure simultaneously multiple distances between 5-fluorotryptophan and a RET acceptor. We demonstrate that for an oligomeric protein, consisting of two single-tryptophan-containing subunits, the individual distances between 5-fluorotryptophan and the single substrate binding site can be resolved using a substrate harboring a RET acceptor.