Measuring the radiation exposure of Norwegian reindeer under field conditions.

Models and approaches have been developed to predict radiation exposure of wildlife under field conditions. However, there have been few attempts to directly measure radiation exposure of wildlife in the field and confirm the doses predicted by models. This is a potential issue for stakeholder acceptance of modelling-based assessments. Here is presented a comprehensive study comparing the results of different dosimeters fitted to free-ranging reindeer inhabiting an area that received comparatively high radiocaesium deposition from the 1986 Chernobyl accident. The external dose of reindeer was measured using the four dosimeter types in aluminium box mounted on the GPS collar. The measurements were compared with two model predictions: (i) external dose to reindeer across the entire range area of the herd; and (ii) external doses of individual reindeer predicted using GPS tracking data to determine locations. It was found that although significant differences between the estimates of the various dosimeters were found these were small with no practical implication. Also, the mean predicted external doses using the GPS tracking data were not significantly different to estimates from two of the four passive dosimeter results. The average external dose predicted across the herd area was significantly lower than doses recorded by the dosimeters and also estimates using GPS data to determine reindeer location (and hence exposure). For 137Cs the average external dose from the GPS tracking data was about twice that predicted across the herd area, because collared animals favoured the more contaminated area of the study site. This suggests that in some circumstances the assumption of averaging contamination over an assumed home range within assessments may be inadequate though this would need to be balanced against other uncertainties. Natural radiation was the greatest contribution to reindeer exposure and a function of the high altitude.

Radiation effects and ecological processes in a freshwater microcosm.

Ecosystem response to gamma radiation exposure depends on the different species sensitivities and the multitude of direct and indirect pathways by which individual organisms can be affected, including the potential for complex interactions across multiple trophic levels. In this study, multi-species microcosms were used to investigate effects of ionizing radiation in a model freshwater ecosystem, including endpoints at both structural and functional levels and ecological interactions. Microcosms were exposed for 22 days to a gradient of gamma radiation with four dose rates from 0.72 to 19 mGy h-1, which are within the range of those seen at contaminated sites. Results showed significant dose related effects on photosynthetic parameters for all macrophyte species. No significant effects of radiation were observed for the consumers in the microcosms, however trends indicate the potential for longer-term effects. We also witnessed a different response of Daphnia magna and Lemna minor compared to previous single-species studies, illustrating the importance of multispecies studies, which aim to encompass systems more realistic to natural ecosystems. Microcosms allowed us to isolate specific relationships between interacting species in an ecosystem and test the effects, both direct and indirect, of radiation on them. In addition, the ecological pathways and processes, and the experimental design itself, was central to understanding the results we witnessed. This type of study is important for radioecology research that has been very much limited to high dose rates and single species studies. This approach to radioecology has been strongly promoted in recent decades and, to our knowledge, this is the first microcosm study performed at dose rates similar to those at contaminated field sites.

Population genetic analysis reveals barriers and corridors for gene flow within and among riparian populations of a rare plant.

Landscape features and life-history traits affect gene flow, migration and drift to impact on spatial genetic structure of species. Understanding this is important for managing genetic diversity of threatened species. This study assessed the spatial genetic structure of the rare riparian Grevillea sp. Cooljarloo (Proteaceae), which is restricted to a 20 km2 region impacted by mining in the northern sandplains of the Southwest Australian Floristic Region, an international biodiversity hotspot. Within creek lines and floodplains, the distribution is largely continuous. Models of dispersal within riparian systems were assessed by spatial genetic analyses including population level partitioning of genetic variation and individual Bayesian clustering. High levels of genetic variation and weak isolation by distance within creek line and floodplain populations suggest large effective population sizes and strong connectivity, with little evidence for unidirectional gene flow as might be expected from hydrochory. Regional clustering of creek line populations and strong divergence among creek line populations suggest substantially lower levels of gene flow among creek lines than within creek lines. There was however a surprising amount of genetic admixture in floodplain populations, which could be explained by irregular flooding and/or movements by highly mobile nectar-feeding bird pollinators. Our results highlight that for conservation of rare riparian species, avoiding an impact to hydrodynamic processes, such as water tables and flooding dynamics, may be just as critical as avoiding direct impacts on the number of plants.

Long-term genetic monitoring reveals contrasting changes in the genetic composition of newly established populations of the intertidal snail Bembicium vittatum.

Newly established populations are susceptible to founder events that reduce genetic variation. This may be counterbalanced by gene flow after populations become established or founders coming from genetically different populations. However, initial gains in genetic diversity may be short-lived if there is limited mixing between lineages and subsequent inbreeding, or if one lineage sweeps to fixation through selection or genetic drift. Here, we report on the genetic changes taking place within two newly established populations of intertidal snail over a 15-year period (∼ 10 generations). Each translocation was set up using multiple, genetically distinct source populations. Our data show that higher levels of variation in the translocated populations compared to the source populations were maintained over time for both nuclear (microsatellite) and mitochondrial genes. Small changes in allele and haplotype frequencies were observed in the source populations and in one of the translocated populations, but marked changes were evident in the other, where there was a dramatic shift towards the genetic make-up of one of the source populations. These genetic changes occurred despite relatively large numbers of founders (200-374 adults) and no evidence of the population experiencing a severe reduction in effective population size. Our study shows that the genetic composition of newly established populations can vary greatly over time and that genetic outcomes can be highly variable, and significantly different from initial expectations, even when they are established using high numbers of individuals and involve source populations from the same geographic regions.