RESUMO
SRI (sensory rhodopsinâ I) can discriminate multiple colors for the attractant and repellent phototaxis. Studies aimed at revealing the color-dependent mechanism show that SRI is a challenging system not only in photobiology but also in photochemistry. During the photoreaction of SRI, an M-intermediate (attractant) transforms into a P-intermediate (repellent) by absorbing blue light. Consequently, SRI then cycles back to the G-state. The photoreactions were monitored with the (13)Câ NMR signals of [20-(13)C]retnal-SrSRI using inâ situ photo-irradiation solid-state NMR spectroscopy. The M-intermediate was trapped at -40 °C by illumination at 520â nm. It was transformed into the P-intermediate by subsequent illumination at 365â nm. These results reveal that the G-state could be directly transformed to the P-intermediate by illumination at 365â nm. Thus, the stationary trapped M- and P-intermediates are responsible for positive and negative phototaxis, respectively.
Assuntos
Espectroscopia de Ressonância Magnética , Rodopsinas Sensoriais/química , Isótopos de Carbono/química , Luz , Retina/metabolismo , TemperaturaRESUMO
A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The (13)C NMR signals from [20-(13)C]retinal-ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.