FT-IR-based fingerprint combined with unsupervised chemometric analysis revealed particle sizes and aqueous-ethanol ratio alter the chemical composition and nutraceutical value of Daucus carota.

This study reported the effects of particle size of dry powder and the optimum solvent extraction on the nutraceutical value of carrot by observing its FT-IR fingerprint, TPC, TFC, and antioxidant activity. The dried-powdered carrot was ultrasound-assisted extracted using EtOH, water, and EtOH-water. The TFC, TPC, and antioxidant activity were analysed by a colorimetric method using a spectrophotometry UV-Vis. The chemotaxonomy of samples was analysed using FT-IR combined with chemometrics analysis. The TFC, TPC, and antioxidant capacity were significantly different for each sample with the highest TFC, TPC, and antioxidant obtained on particle size 149 µm with EtOH-water (50:50) as the most prominent solvent (19.51 mgQE/g, 9.90 mgGAE/g, IC50: 16.7 ± 0.89 µg/mL). FT-IR profiling of samples also illustrates a minor different pattern of the spectrum, indicating there is a difference in their chemical composition. The particle size and EtOH-water ratio influence the chemical composition and antioxidant activity of carrots.

The Bemisia tabaci species complex: additions from different parts of the world.

Bemisia tabaci is one of the most threatening pests in many crops. We sequenced part of the mitochondrial cytochrome oxidase I gene from fifty whitefly populations collected in Indonesia, Thailand, India and China. Nineteen unique sequences (haplotypes) of the cytochrome oxidase I were identified in these populations. They were combined with sequences available in databases, resulting in a total of 407 haplotypes and analyzed together with nine outgroup accessions. A phylogenetic tree was calculated using the maximum likelihood method. The tree showed that all groups that were found in previous studies were also present in our study. Additionally, seven new groups were identified based on the new haplotypes. Most B. tabaci haplotypes grouped based on their geographical origin. Two groups were found to have a worldwide distribution. Our results indicate that our knowledge on the species complex around B. tabaci is still far from complete.

Identification and QTL mapping of whitefly resistance components in Solanum galapagense.

Solanum galapagense is closely related to the cultivated tomato and can show a very good resistance towards whitefly. A segregating population resulting from a cross between the cultivated tomato and a whitefly resistant S. galapagense was created and used for mapping whitefly resistance and related traits, which made it possible to study the genetic basis of the resistance. Quantitative trait loci (QTL) for adult survival co-localized with type IV trichome characteristics (presence, density, gland longevity and gland size). A major QTL (Wf-1) was found for adult survival and trichome characters on Chromosome 2. This QTL explained 54.1 % of the variation in adult survival and 81.5 % of the occurrence of type IV trichomes. A minor QTL (Wf-2) for adult survival and trichome characters was identified on Chromosome 9. The major QTL was confirmed in F3 populations. Comprehensive metabolomics, based on GCMS profiling, revealed that 16 metabolites segregating in the F2 mapping population were associated with Wf-1 and/or Wf-2. Analysis of the 10 most resistant and susceptible F2 genotypes by LCMS showed that several acyl sugars were present in significantly higher concentration in the whitefly resistant genotypes, suggesting a role for these components in the resistance as well. Our results show that whitefly resistance in S. galapagense seems to inherit relatively simple compared to whitefly resistance from other sources and this offers great prospects for resistance breeding as well as elucidating the underlying molecular mechanism(s) of the resistance.