RESUMO
A total of 4,111 chicken sera collected from 1985 to 1995 at 137 farms in 36 prefectures were subjected to an enzyme-linked immunosorbent assay (ELISA) to test for antibodies against turkey rhinotracheitis (TRT) virus. The antibodies to TRT virus were detected in the chicken sera collected from 1988 to 1995, but the antibodies to TRT virus were undetectable in the sera of chickens collected before 1987. The antibody positive rates each year ranged from 2.2% (1988) to 54.2% (1995). Recently, the TRT virus has spread over the flocks of commercial broiler and layer chicken throughout the country. The serological findings indicated that the TRT virus had invaded the chickens before 1988, and has been widespread thereafter in Japan.
Assuntos
Anticorpos Antivirais/sangue , Infecções por Pneumovirus/veterinária , Doenças das Aves Domésticas/epidemiologia , Animais , Galinhas , Ensaio de Imunoadsorção Enzimática , Reações Falso-Negativas , Reações Falso-Positivas , Geografia , Japão/epidemiologia , Testes de Neutralização , Pneumovirus/imunologia , Infecções por Pneumovirus/epidemiologia , Reprodutibilidade dos TestesRESUMO
Two monoclonal antibodies (mAbs) with neutralizing activities directed to the fusion (F) 1 glycoprotein of the 8597/CV94 strain of turkey rhinotracheitis (TRT) virus inhibited cell fusion by TRT virus in vitro. In contrast, F1-specific mAbs with no neutralizing activity did not inhibit the cell fusion participating in syncytium formation. These findings indicate that the neutralization epitope is the same as or overlaps the fusion active site.
