RESUMO
Nutrient partitioning was investigated in cows with different genetic merits for milk production by measuring (13)C/(12)C ratios (reported by delta values δ(13)C) in milk components in response to C3 (grass silage) and C4 diets (corn silage). We hypothesised that changes of δ(13)C in milk differ between Holstein (HOL; high milk production) and Charolais × Holstein cows with medium (CHM) and low (CHL) milk production. Changes of δ(13)C (Δδ(13)C) in milk components were estimated by calculating differences of δ(13)C due to switch from C3 to C4 feeding. After switch to C4 feeding, Δδ(13)C of lactose was greater in HOL than in CHL. Immediate Δδ(13)C of milk fat was the lowest in CHL. The maximal Δδ(13)C of casein was the lowest in HOL. The proportion of carbon in milk derived from diet increased with milk yield, indicating the main impact of the milk production level, but minor impact of breed, on nutrient partitioning towards the mammary gland.