RESUMO
Terrestrial anuran amphibians absorb water across specialized regions of skin on the posterioventral region of their bodies. Rapid water absorption is mediated by the insertion of aquaporins into the apical membrane of the outermost cell layer. Water moves out of the epithelium via aquaglyceroporins in the basolateral membrane and into the circulation in conjunction with increased capillary blood flow to the skin and aquaporins in the capillary endothelial cells. These physiological responses are activated by intrinsic stimuli relating to the animals' hydration status and extrinsic stimuli relating to the detection of osmotically available water. The integration of these processes has been studied using behavioural observations in conjunction with neurophysiological recordings and studies of epithelial transport. These studies have identified plasma volume and urinary bladder stores as intrinsic stimuli that activate the formation of angiotensin II (AII) to stimulate water absorption behaviour. The coordinated increase in water permeability and capillary blood flow appears to be mediated primarily by sympathetic stimulation of beta adrenergic receptors, although the neurohypopyseal hormone arginine vasotocin (AVT) may also play a role. Extrinsic stimuli relate primarily to the ionic and osmotic properties of hydration sources. Toads avoid NaCl solutions that have been shown to be harmful in acute exposure, approx. 200-250 mm. The avoidance is partially attenuated by amiloride raising the hypothesis that the mechanism for salt detection by toads resembles that for salt taste in mammals that take in water by mouth. In this model, depolarization of the basolateral membrane of taste cells is coupled to afferent neural stimulation. In toad skin we have identified innervation of skin epithelial cells by branches of spinal nerves and measured neural responses to NaCl solutions that elicit behavioural avoidance. These same concentrations produce depolarization of the basolateral membrane in isolated epithelial preparations. As with salt taste in mammals, the neural responses and depolarization of basolateral membrane potential are partially inhibited by amiloride. In addition, toads are more tolerant of sodium gluconate solution which is consistent with the phenomenon in mammalian taste physiology termed the anion paradox in which sodium salts with larger molecular weight anions produce a reduced intensity of salt taste. Finally, toads also avoid concentrated solutions of a non-electrolyte, mannitol, which differs from NaCl solutions in not affecting transepithelial conductance and requires a longer time to depolarize the basolateral membrane. Osmotic stimuli may mediate sensory processes for longer term detection of conditions with low water potential while ionic stimuli are more important for shorter term analysis of rehydration sources.
Assuntos
Anfíbios/anatomia & histologia , Transporte Biológico/fisiologia , Capilares/metabolismo , Células Quimiorreceptoras/metabolismo , Fluxo Sanguíneo Regional/fisiologia , Fenômenos Fisiológicos da Pele , Pele/irrigação sanguínea , Pele/citologia , Animais , Comportamento Animal/fisiologia , Epitélio/fisiologia , Humanos , Concentração Osmolar , Água/metabolismoRESUMO
Blood cell flux (BCF) in ventral pelvic skin capillaries was measured in toads, Bufo woodhouseii and Bufo punctatus, using a chamber that allowed hydration behavior and water absorption to be observed concurrently in unrestrained animals. Dehydrated B. woodhouseii and B. punctatus placed on a rehydration solution significantly increased BCF relative to that on a dry surface in less than 2 min. Skin contact with a rehydration solution rather than dehydration alone is the primary stimulus for increased seat patch blood flow. In B. woodhouseii, the water absorption response was initiated after the increase in BCF had started but before maximum BCF was reached. BCF and water uptake across the ventral skin of both species placed on deionized water were not different from those of toads placed on 50 mM NaCl. Similarly, no significant correlation between BCF and rate of water uptake could be observed in dehydrated toads of either species. Angiotensin II (AII) injection in hydrated B. punctatus had no effect on BCF, suggesting that factors other than AII are responsible for the increase in blood flow upon water contact in dehydrated toads.
Assuntos
Água Corporal/metabolismo , Bufonidae/metabolismo , Desidratação/metabolismo , Pele/irrigação sanguínea , Análise de Variância , Angiotensina II , Animais , Transporte Biológico/fisiologia , Células Sanguíneas/metabolismo , Capilares/metabolismo , Lasers , Fluxo Sanguíneo Regional/fisiologia , Fatores de TempoRESUMO
Toads, Bufo marinus, allowed to maintain an ad libitum state of hydration were dehydrated by 10 15% of their standard weight and allowed to rehydrate from either deionized water or from 10 or 50 mmol l(-1) NaCl solutions. Toads rehydrating from the dilute salt solutions recovered a larger fraction of their standard weight than did toads rehydrating from deionized water despite there being a reduced osmotic gradient. Amiloride did not reduce water gain from these solutions. Water uptake from 100 mmol l(-1) sucrose and 50 mmol l(-1) Na gluconate was reduced relative to deionized water by a fraction predicted from the osmotic gradient. Thus, the presence of both Na+ and Cl- are required for the augmentation of water gain from dilute salt solutions. Toads allowed to rehydrate from 120 mmol l(-1) NaCl for 180 min recovered nearly as much water as toads rehydrating from deionized water for 120 min and the lymph osmolality was not reduced relative to the dehydrated condition. The recovery of water from the salt solution was greater than that predicted from the reduced osmotic gradient and amiloride partially inhibited the rehydration from 120 mmol l(-1) NaCl. Solute coupled water transport can therefore be demonstrated in living animals but only from a NaCl solution that is nearly isoosmotic with the lymph. The mechanism for enhanced water gain from dilute salt solutions remains unresolved.
Assuntos
Bufo marinus/fisiologia , Hidratação , Linfa/efeitos dos fármacos , Linfa/metabolismo , Cloreto de Sódio/farmacologia , Amilorida/farmacologia , Animais , Diuréticos/farmacologia , Concentração OsmolarRESUMO
Toads normally obtain water by absorption across their skin from osmotically dilute sources. When hyperosmotic salt solutions are presented as a hydration source to dehydrated desert toads, they place the ventral skin onto the source but soon afterwards escape to avoid dehydration. The escape behavior coincides with neural excitation of the spinal nerves that innervate putative chemosensory cells in the ventral skin. In the present study, fluorescent dye translocated through the spinal nerves to those receptor cells in the epidermis was photoconverted in the presence of 3, 3'-diaminobenzidine tetrahydrochloride for electron-microscopic observation of the cells and associated nerve terminals. Most of the photoconverted cells were located in the deepest layer of the epidermis, with some being in more intermediate layers. No labeled cell was seen in the outermost layer of living cells. In desert toads, flask cells and Merkel cells are occasionally seen in the epidermis. An association of nerve fibers with these epidermal cells has been reported in some species of the anurans. In the present study, however, the cytological features of the photoconverted cells are neither reminiscent of flask cells nor Merkel cells, but are similar to those of surrounding epithelial cells in each layer of the epidermis. We hypothesize a sensory function for these cells, because they have a close association with nerve fibers and participate in the transepithelial transport of salts that must pass through all cell layers of the skin.
Assuntos
Bufonidae/anatomia & histologia , Células Quimiorreceptoras/efeitos dos fármacos , Pele/inervação , Nervos Espinhais/citologia , Animais , Carbocianinas , Células Quimiorreceptoras/citologia , Células Quimiorreceptoras/ultraestrutura , Corantes Fluorescentes , Microscopia Eletrônica , Pele/citologia , Pele/ultraestrutura , Nervos Espinhais/ultraestrutura , Distribuição TecidualRESUMO
Amphibians absorb water osmotically across their skins and rely on chemosensory information from the skin to assess the suitability of hydration sources. The time spent with skin in contact with a moist surface provides a quantitative measure of their ability to perceive the ionic and osmotic properties of aqueous solutions. Dehydrated toads given hyperosmotic (250 mM) solutions of NaCl or Na-gluconate showed significantly longer periods of hydration behavior on the gluconate solution, but they lost water osmotically when immersed in either solution. Similarly, dehydrated toads given 250 mM solutions of NaCl, Na-acetate, Na-phosphate or Na-gluconate showed a progressively greater length of hydration time on solutions with the larger mol. wt anions. These results are consistent with the chemosensory phenomenon previously described in mammalian tongue as 'anion paradox'. On dilute (50 mM) solutions of NaCl or Na-gluconate, the hydration time was not different between anions, despite toads gaining water more rapidly when immersed in dilute NaCl than in Na-gluconate solutions. The differing behavioral results with hyperosmotic and hypoosmotic salt solutions suggest that chemosensory transduction through toad skin involves both transcellular and paracellular pathways.
Assuntos
Bufonidae/fisiologia , Desidratação/tratamento farmacológico , Soluções para Reidratação/química , Soluções para Reidratação/farmacologia , Fenômenos Fisiológicos da Pele , Animais , Ânions , Transporte Biológico , Cloretos/metabolismo , Sódio/metabolismo , Sódio/fisiologia , Cloreto de Sódio/farmacologia , Paladar/fisiologia , Água , Aumento de PesoRESUMO
Toads, Bufo marinus, were placed on laboratory tissue saturated with water or with hyperosmotic (250 or 500 mM NaCl or KCl) solutions, and their behavior was observed for 5 min. Toads placed on water initially allowed their ventral skin to touch the surface without abducting the hind limbs. During this "seat patch down" (SPD) behavior toads appeared to be evaluating the suitability of a hydration source prior to initiating "water absorption response" (WR) behavior with the hind limbs fully abducted and the ventral skin pressed to the moist surface. Toads dehydrated by more than 10% showed significantly shorter periods of SPD behavior and initiated WR behavior more frequently than did hydrated toads. Dehydrated toads placed on 250 mM NaCl initiated WR behavior in only 18% of the trials, but spent significantly more time showing SPD behavior than they did on water, indicating that this concentration is marginally acceptable to them. Recordings from spinal nerve #6 showed an increase in activity when 250 mM NaCl or KCl solutions were perfused over the outer surface of the ventral skin. The response to KCl was significantly greater than NaCl. The addition of 10 microM amiloride to 250 mM NaCl resulted in a higher frequency of WR behavior and reversibly inhibited the neural response to 250 mM NaCl. These results suggest that epithelial Na+ channels in the skin serve a sensory function in this species. Neither the hydrated nor dehydrated toads initiated WR behavior on 250 or 500 mM KCl solutions, indicating that toads have a lower tolerance of K+ than of Na+ salts.
Assuntos
Comportamento Animal/efeitos dos fármacos , Células Quimiorreceptoras/efeitos dos fármacos , Cloreto de Potássio/farmacologia , Solução Salina Hipertônica/farmacologia , Amilorida/farmacologia , Animais , Bufo marinus , Desidratação/psicologia , Diuréticos/farmacologia , Concentração Osmolar , Transdução de Sinais/fisiologia , Nervos Espinhais/fisiologiaRESUMO
Amphibian water balance has been studied at many levels of biological order. Terrestrial species must react to environmental cues that relate to water availability while some arboreal species have cutaneous skin secretions that can reduce evaporative water loss. The Indian tree frog. Polypedates maculatus, uses cutaneous secretions and wiping behavior to lower evaporation but also relies on moist microclimates to endure prolonged survival away from water. The related species, P. leucomystax, inhabits wetter forest habitats. Preliminary studies with this species are unable to demonstrate the expression of wiping behavior, indicating that arid habitats may be a powerful selective force for this behavior. Laboratory experiments on rehydrating toads in the genus Bufo indicate that animals are able to detect changes in barometric pressure and humidity that might result in the availability of water under field situations. Experiments with Bufonid species and with spadefoot toads, Scaphiopus couchi, show that the peptide hormone, angiotensin II, stimulates cutaneous drinking in a similar manner seen for oral drinking by other vertebrate classes. Amphibian tissues have long been used as a model for the study of basic physiological principles of epithelial ion and water transport. Recent progress with tissue cultures has provided information on the molecular structure of ion and water channels that can be applied to obtain a better understanding, at the molecular level, of ion and water balance strategies used by the wide variety of amphibian species. Terrestrial amphibians are more tolerant of dehydration than are other vertebrates and are able to store dilute urine in their urinary bladder. Toads appear to be able to detect the presence of water in their bladders in addition to the availability of water in their environment. Dehydrated toads are able to rehydrate very rapidly by the coordination of behavioral and physiological mechanisms to enhance cutaneous water absorption. The integration of behavior with cutaneous water gain, renal handling of ions and water and the role of the lymphatic system in overall water balance involves complex interactions between neural and hormonal factors. Experiments are summarized that describe the contribution of individual factors however much more information is needed before the nature of these interactions are fully understood.
Assuntos
Anuros/fisiologia , Desidratação , Equilíbrio Hidroeletrolítico , Adaptação Fisiológica , Animais , Meio AmbienteRESUMO
Toads obtain water by absorption across their skin. When dehydrated, desert toads exhibit stereotyped hydration behavior in which they press their ventral skin onto a moist surface. However, dehydrated toads avoid surfaces moistened with hyperosmotic NaCl and KCl solutions (Hoff KvS, Hillyard SD. 1993. J. Exp. Biol. 183:347-351). We have studied neural mechanisms for this avoidance with physiologic, behavioral, and morphologic approaches. Spinal nerves innervating the ventral skin could be stimulated by exposure to a hyperosmotic NaCl solution applied to the outer surface of the skin. This neural response occurred with much longer latency than to mechanical stimulation and could be reduced by amiloride, a blocker for Na+ channels known to be responsible for epithelial ion transport and salt taste transduction. In behavioral experiments, avoidance of a NaCl solution was also reduced by adding amiloride to the solution, suggesting involvement of amiloride-sensitive Na+ channels for detecting the hyperosmotic salt solution. Neural tracing with fluorescent dye revealed spinal nerve endings and connections to putative receptor cells, both located in the deeper layer of the epidermis. Either of these or both may be associated with the transduction of Na+ flowing into the skin. The ability of toads to detect hyperosmotic salt solutions in their environment reveals a previously unknown chemosensory function for spinal nerves in anuran amphibians.
Assuntos
Bufonidae/fisiologia , Células Quimiorreceptoras/fisiologia , Absorção Cutânea , Nervos Espinhais/fisiologia , Água , Potenciais de Ação/efeitos dos fármacos , Animais , Células Quimiorreceptoras/efeitos dos fármacos , Desidratação , Sais/farmacologia , Canais de Sódio/efeitos dos fármacos , Nervos Espinhais/citologia , Nervos Espinhais/ultraestruturaRESUMO
Terrestrial amphibians take up water by abducting the hind limbs and pressing a specialized portion of the ventral skin to a moist surface, using a characteristic behavior called the water absorption response. An assay of the water absorption response was used to quantify physiological factors associated with thirst and water uptake. Dramatic changes in the water absorption response resulted from subtle changes in hydration state and from altering the reserve water supply in the urinary bladder. The water absorption response could be induced by intraperitoneal and intracerebroventricular injection of angiotensin II, demonstrating that components of the renin-angiotensin system on both sides of the blood-brain barrier have a dipsogenic function in amphibians. These experiments also demonstrated that the water absorption response could be influenced by changes in barometric pressure. Toads avoided the water absorption response on hyperosmotic substrates, and behavioral experiments showed that the amphibian skin served a sensory function similar to that of the lingual epithelium of mammals. The water absorption response assay has enormous potential as a tool for the investigation of physiological processes and sensory capabilities of amphibians.
Assuntos
Anfíbios/fisiologia , Comportamento Animal/fisiologia , Movimento , Equilíbrio Hidroeletrolítico , Água/metabolismo , Absorção , Angiotensina II/administração & dosagem , Animais , Desidratação , Osmose , Sistema Renina-Angiotensina/fisiologia , PeleRESUMO
Skin from larval bullfrogs was mounted in an Ussing-type chamber in which the apical surface was bathed with a Ringer solution containing 115 mM K+ and the basolateral surface was bathed with a Ringer solution containing 115 mM Na+. Ion transport was measured as the short-circuit current (Isc) with a low-noise voltage clamp, and skin resistance (Rm) was measured by applying a direct current voltage pulse. Membrane impedance was calculated by applying a voltage signal consisting of 53 sine waves to the command stage of the voltage clamp. From the ratio of the Fourier-transformed voltage and current signals, it was possible to calculate the resistance and capacitance of the apical and basolateral membranes of the epithelium (Ra and Rb, Ca and Cb, respectively). With SO4(2-) as the anion, Rm decreased rapidly within 5 min following the addition of 150 U/ml nystatin to the apical solution, whereas Isc increased from 0.66 to 52.03 microA/cm2 over a 60-min period. These results indicate that nystatin becomes rapidly incorporated into the apical membrane and that the increase in basolateral K+ permeability requires a more prolonged time course. Intermediate levels of Isc were obtained by adding 50, 100, and 150 U/ml nystatin to the apical solution. This produced a progressive decrease in Ra and Rb while Ca and Cb remained constant. With Cl- as the anion, Isc values increased from 2.03 to 89.57 microA/cm2 following treatment with 150 U/ml nystatin, whereas with gluconate as the anion Isc was only increased from 0.63 to 11.64 microA/cm2. This suggests that the increase in basolateral K+ permeability produced by nystatin treatment, in the presence of more permeable anions, is due to swelling of the epithelial cells of the tissue rather than the gradient for apical K+ entry. Finally, Cb was not different among skins exposed to Cl-, SO4(2-), or gluconate, despite the large differences in Isc, nor did inhibition of Isc by treatment with hyperosmotic dextrose cause significant changes in Cb. These results support the hypothesis that increases in cell volume activate K+ channels that are already present in the basolateral membrane of epithelial cells.
Assuntos
Membrana Celular/fisiologia , Potássio/metabolismo , Fenômenos Fisiológicos da Pele , Animais , Ânions/farmacologia , Membrana Celular/efeitos dos fármacos , Técnicas In Vitro , Larva , Potenciais da Membrana/efeitos dos fármacos , Modelos Biológicos , Mucosa/fisiologia , Nistatina/farmacologia , Técnicas de Patch-Clamp , Rana catesbeianaRESUMO
Angiotensin II (A-II), a potent inducer of thirst-related behavior in many vertebrate species, was injected into the third ventricle of the brain of the spadefoot toad, Scaphiopus couchii. Following injection of 10 ng A-II the animals demonstrated a significant increase in water absorption response (WR) behavior, in which toads press their ventral skin to a moist surface and absorb water by osmosis. This increase in the frequency of WR behavior was positively correlated with an increase in water gain during a 2-hr period indicating that centrally injected A-II stimulates water intake by this amphibian species. We have previously demonstrated that WR behavior is also induced by intraperitoneal (i.p.) injections of A-II in several anuran species, including S. couchii. Thus, amphibians, like other vertebrates, demonstrate an increase in water intake in response to either centrally administered or circulating A-II. A second series of experiments was conducted to determine whether the above response to A-II might be secondary to increases in the circulating levels of aldosterone (ALDO) or antidiuretic hormone because the release of both of these hormones has been shown by others to be stimulated by A-II. Scaphiopus couchii injected i.p. with either ALDO or arginine vasotocin in dosages of 1, 10, and 100 micrograms/100 g body weight showed no increase in WR behavior relative to toads injected with saline alone. These results suggest that A-II acts directly on the brain of S. couchii to induce WR behavior.
Assuntos
Angiotensina II/farmacologia , Encéfalo/efeitos dos fármacos , Sede/efeitos dos fármacos , Equilíbrio Hidroeletrolítico/efeitos dos fármacos , Aldosterona/farmacologia , Animais , Anuros , Arginina Vasopressina/farmacologia , Ingestão de Líquidos/efeitos dos fármacos , Injeções Intraventriculares , Absorção Cutânea/efeitos dos fármacosRESUMO
1. Water absorption response (WR) behavior and water weight gain were examined in hydrated toads, Bufo woodhousei, treated with angiotensin II (AII) or with a control Ringer's solution. The effects of urinary bladder condition (ad lib. bladder urine or empty bladder) were examined concurrently. 2. Toads treated with AII (100 micrograms/100 g body weight), spent more time in WR posture and absorbed more water than Ringer's-injected toads. 3. Toads with empty bladders maintained WR posture for longer periods of time and gained more weight than toads whose bladders were not emptied. 4. The effects of AII and bladder urine on water absorption by B. woodhousei appear to be separate and additive.
Assuntos
Angiotensina II/farmacologia , Bufonidae/metabolismo , Absorção Cutânea/efeitos dos fármacos , Bexiga Urinária/efeitos dos fármacos , Água/metabolismo , Animais , Bufonidae/urinaRESUMO
The short-circuit current (Isc) across isolated skin from larval frogs (Rana catesbeiana) was measured when the tissue was bathed with Na2SO4 Ringer solution on the serosal side and with a Ringer solution containing K+ as the primary cation on the mucosal side. When 150 U/ml nystatin was added to the mucosal solution, the Isc increased from 1.4 +/- 0.1 to 35.4 +/- 4.8 microA/cm2. When verapamil was added to the mucosal and serosal Ringer solutions in concentrations between 2.5 and 80 microM, Isc was inhibited in a stepwise manner. At 80 microM, Isc was reduced by 75.3% to 8.74 +/- 1.14 microA/cm2. Analysis of the inhibition of Isc with the direct linear plot method showed that the blockage of Isc could be described by pseudo-first-order kinetics with a Michaelis constant (Km) of 9.59 +/- 2.20 microM. Fluctuation analysis revealed a Lorentzian component in power spectra obtained from preparations treated with 10-80 microM verapamil. The corner frequency of these Lorentzian components increased in a linear manner over this range of verapamil concentrations. The Km calculated from the ratio of the dissociation and association rate constants (k10/k'01) was 39.5 microM. The single-channel currents (i) calculated from the fluctuation analysis parameters decreased significantly between verapamil concentrations of 10 and 80 microM. It appears that the inhibition of K+ channels in the basolateral membrane of this tissue has at least two components.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Canais de Potássio/efeitos dos fármacos , Rana catesbeiana/metabolismo , Pele/metabolismo , Verapamil/farmacologia , Animais , Eletrofisiologia , Larva , Nistatina/farmacologia , Concentração Osmolar , Canais de Potássio/metabolismo , Canais de Potássio/fisiologia , Rana catesbeiana/fisiologia , Pele/efeitos dos fármacos , Fenômenos Fisiológicos da PeleRESUMO
The amphibian antidiuretic hormone, arginine vasotocin, stimulated osmotic water flow across isolated skin from the pelvic but not the pectoral skin of the toad, Bufo woodhouseii. Changes in the apical membrane capacitance were not observed for either region of the skin following treatment with arginine vasotocin when there was an osmotic gradient across the tissue. In the absence of an osmotic pressure gradient, the apical membrane capacitance of the pelvic skin increased from 2.8 +/- 0.5 to 3.3 +/- 0.6 microF.cm-2 after treatment with 5 x 10(-8) M arginine vasotocin. Under these conditions, apical membrane capacitance of the pectoral skin was 1.8 +/- 0.1 microF.cm-2 and did not change significantly after arginine vasotocin treatment. The amiloride-sensitive short-circuit current across the pelvic skin was stimulated by arginine vasotocin as was the density of channels in the apical membrane as determined by fluctuation analysis. Values for channel density in the pelvic skin also correlated with apical membrane capacitance and increased from 90 to 273 channels per micron2 of estimated membrane area following arginine vasotocin treatment. In the pectoral skin the stimulation of short-circuit current following arginine vasotocin treatment was small and an increase in channel density could not be demonstrated. The current through single Na+ channels in both regions of the skin did not different either before or after arginine vasotocin treatment.
Assuntos
Eletrofisiologia , Osmose/efeitos dos fármacos , Fenômenos Fisiológicos da Pele , Sódio/metabolismo , Vasotocina/farmacologia , Amilorida/farmacologia , Animais , Bufonidae , Transporte de Íons/efeitos dos fármacos , Pele/efeitos dos fármacosRESUMO
The short-circuit current (SCC) across isolated skin from bullfrog larvae in developmental stage XXI was small and insensitive to amiloride. Overnight incubation of this tissue with 10(-6) M aldosterone stimulated the SCC from 1.35 +/- 0.55 to 14.55 +/- 4.12 microA.cm-2 with 11.18 +/- 4.46 microA.cm-2 being blocked by 100 microM amiloride. Histologic examination of aldosterone-treated skins revealed a separation of the apical cell layer from the underlying epidermis that was not seen in untreated preparations. The onset of amiloride-sensitive Na+ transport thus coincided with the exposure of the apical surface of newly differentiated epithelial cells. Similar results were obtained with skin from stage XXI larvae whose rate of metamorphosis had been stimulated by 10 micrograms.1-1 thyroxine (T4) but not with skin from T4-treated larvae in stages XIX and XX. Fluctuation analysis of the amiloride-sensitive SCC of the above preparations failed to show a consistent Lorentzian component in the power-density spectrum. Fluctuation analysis was possible on skins from larvae whose development had been accelerated by 7-9 days treatment with 10 micrograms.l-1 triiodothyronine (T3). Aldosterone treatment of these tissues resulted in a significant increase in Na+ channel density.
Assuntos
Aldosterona/farmacologia , Rana catesbeiana/fisiologia , Pele/efeitos dos fármacos , Amilorida/farmacologia , Animais , Eletrofisiologia , Larva , Metamorfose Biológica/efeitos dos fármacos , Pele/anatomia & histologia , Fenômenos Fisiológicos da Pele , Canais de Sódio/efeitos dos fármacos , Canais de Sódio/metabolismo , Tiroxina/farmacologiaRESUMO
A small, inward-directed, short-circuit current (SCC) was measured across the isolated skin of larval bullfrogs (Rana catesbeiana) when either NaCl or KCl Ringer solution bathed the mucosal surface. The addition of amiloride, in concentrations of 1-100 microM, produced a stepwise increase in SCC. As SCC values became maximally elevated by amiloride, the plateau value (So) of the Lorentzian component in the power-density spectrum increased, whereas the corner frequency (fc) decreased. This agonist effect of amiloride can be explained by an increase in the open probability and possibly the single-channel current of the larval channel. When the amiloride concentration was increased above 100 microM, the SCC values declined progressively but usually remained above pretreatment values. This suggests an antagonist effect of amiloride that is concurrent with the agonist effect. The removal of Ca2+ from the mucosal Ringers increased SCC in conjunction with an increase in So and a decrease in fc. Under these conditions, the maximal agonist effect of amiloride was observed at concentrations of 10-20 microM. Ca2+ thus exerts an inhibitory effect on the larval cation channel that interferes with the agonist effect of amiloride. The addition of Ba2+ to Ca2+-free preparations lowered SCC and reduced the agonist effect of amiloride.
Assuntos
Amilorida/farmacologia , Canais Iônicos/fisiologia , Fenômenos Fisiológicos da Pele , Animais , Bário/farmacologia , Cálcio/farmacologia , Cálcio/fisiologia , Cátions , Condutividade Elétrica , Canais Iônicos/efeitos dos fármacos , Larva/fisiologia , Potássio/farmacologia , Rana catesbeiana , Sódio/farmacologiaRESUMO
The skin of frog larvae (Rana catesbeiana) was used to study the characteristics of basolateral K+ channels with fluctuation analysis. K2SO4 and Na2SO4 Ringer's were used as mucosal and serosal solution, respectively. After addition of Nystatin (138 U/ml) the transepithelial conductance and short-circuit current (Isc) increased considerably. Most of Isc was carried by K+, moving from the mucosal to the serosal side. This current could be depressed by quinidine, added to both compartments or to mucosal side only. Fluctuation analysis showed that quinidine induced a Lorentzian component in the power density spectrum. Assuming pseudo-first order kinetics for the channel occlusion by quinidine the current through the open K+ channel and channel density were calculated: iK = 0.22 pA, M = 7.7 channels/microns2.
Assuntos
Canais Iônicos/fisiologia , Quinidina/farmacologia , Fenômenos Fisiológicos da Pele , Animais , Membrana Celular/fisiologia , Condutividade Elétrica/efeitos dos fármacos , Canais Iônicos/efeitos dos fármacos , Cinética , Mucosa/efeitos dos fármacos , Mucosa/fisiologia , Rana catesbeiana , Pele/efeitos dos fármacosRESUMO
Arginine vasopressin (AVP) stimulated active Na+ transport (JNa+) and osmotic water flow (JH2O) across the pelvic skin but only JNa+ across the pectoral skin of the toad, Bufo woodhouseii. Isolated epithelial cells from the pelvic skin had a maximal c-AMP level of 11.16 pmoles/mg protein after 5 min of AVP treatment while that of pectoral skin was 3.64 pmoles/mg protein. The c-AMP level of both skin areas fell to unstimulated values after 20 min of AVP treatment; however, JH2O (pelvic skin) and JNa+ (pelvic and pectoral skin) remained elevated during 3 hr of treatment. Dibutyryl c-AMP and theophylline stimulated JH2O across the pelvic but not the pectoral skin. Maintaining toads in water for 12-24 hr resulted in a substantial lowering of JH2O across the pectoral skin which was not reversible by treatment with c-AMP and theophylline.
Assuntos
Bufonidae/metabolismo , AMP Cíclico/metabolismo , Animais , Epitélio/metabolismo , Permeabilidade , Pele/metabolismo , Sódio/metabolismo , Teofilina/farmacologia , Equilíbrio HidroeletrolíticoRESUMO
Sixty-nine whites (38 men and 31 women) aged 17 to 88 years and 48 blacks (19 men and 29 women) aged 17 to 61 years were studied. Each person walked in desert heat for 1 hour at a rate requiring 40% of aerobic capacity. Observations were recorded on their rectal temperature (Tre), skin temperature (Tsk), heart rate (HR), blood pressure, and sweat rate (SR). Older men and women of both races were able to complete their walks without any ill effects. Age, per se, did not significantly reduce elderly individuals' ability to tolerate the combined stress of dry heat and exercise. Men of both races had higher sweat rate and lower heart rate and rectal and skin temperature than women working at the same percentage of aerobic capacity. Success of thermoregulation at 40% of aerobic capacity of blacks and whites was equal, but in both races men thermoregulated more successful than women. Our data suggest that thermoregulatory capacity of humans under desert conditions differs between sexes and is not influenced significantly by age or race except for differences in aerobic capacity.
Assuntos
Regulação da Temperatura Corporal , Clima Desértico , Temperatura Alta , Adolescente , Adulto , Fatores Etários , Idoso , População Negra , Pressão Sanguínea , Feminino , Frequência Cardíaca , Humanos , Masculino , Pessoa de Meia-Idade , Consumo de Oxigênio , Esforço Físico , Fatores Sexuais , Temperatura Cutânea , Sudorese , População BrancaRESUMO
Many investigators have sought, but failed to find, ethnic differences in the number and regional distribution of active sweat glands. In this study measurements have been made of sweat secreted on one hand and also on the whole body of Whites and Blacks walking in desert heat. Whites numbered 31 men and 27 women, ages 30 to 88 years; there were 21 Black men and 31 Black women, ages 16 to 61 years. Each walked on three occasions for 1 hour at a rate that required an oxygen consumption of about 40% of aerobic capacity. Ambient temperature ranged from 32 to 44 degrees C in 1979 and 1980; means were 38.4 degrees C in 1979 and 36.7 degrees C in 1980. There was no sweat in the gloves of many Blacks; this was true of only a few Whites. Volume of body sweat increased in both races with rate of walking; volume of hand sweat increased more in Whites than in Blacks. The Mann-Whitney test revealed that volumes of hand sweat were significantly greater for Whites than for Blacks. It was concluded that in desert walks most Whites and few Blacks sweat freely on their hands. In samples of hand sweat, Na+, K+, and Cl- were determined. Concentrations of each ion varied widely in both races, and were unrelated to race. Concentrations of Na+ and Cl- generally are somewhat higher in hand sweat than in body sweat; concentrations of K+ are much higher. It follows that the values for concentration of Na+ and Cl- reported in Table 3 probably are somewhat higher than would have been found in body sweat, and concentrations of K+ are probably much higher.