Parametric investigation of static and dynamic cell culture conditions and their impact on hCMEC/D3 barrier properties.

In brain research, the hCMEC/D3 cell line is widely used for the establishment of a human in vitro blood-brain barrier (BBB) model. However, its barrier integrity seems to be insufficient for drug permeability studies, represented by rather low transendothelial electrical resistance (TEER) and high permeability of small molecules. Therefore, this study covers a parametric investigation of static and dynamic cell culture conditions to improve barrier functionality of hCMEC/D3. The effect of basal media was investigated by analyzing changes in proliferation rate, barrier integrity and gene expression of cellular junction proteins. The cells were able to grow in different cell culture media, including serum-free media. However, none of these media enhanced strongly the growth rate or barrier integrity compared to the microvascular endothelial cell growth medium-2 (EGM™-2 MV). Furthermore, hCMEC/D3 cells did not respond positively regarding TEER to any tested parameter neither supplements, coating materials nor co-cultures with the human immortalized astrocyte cell line SVGmm. Furthermore, the impact of dynamic conditions was examined by using the Dynamic Micro Tissue Engineering System (DynaMiTES). Cultivation conditions were successfully adapted to the DynaMiTES design and no negative effect was detected by analyzing cell viability and cell count, albeit TEER remained also unchanged. Consequently, the hCMEC/D3 model has considerable limitations and further improvements or alternative cell lines are required.

Dietary, serum and urine ascorbic acid status in male athletes.

The ascorbic acid (AA)-status of 14 marathon runners, 12 soccer players, 9 wrestlers, 9 basketball players and 16 controls was determined. A 7-day food weighed record was kept to quantify the AA-intake. In addition, the AA-serum concentrations and urinary ascorbate excretion were measured. The AA-intake of all 44 athletes (median, 26th-75th percentile) was 180.7 (188-239) mg/d, the serum concentration 70.6 (65.7-80.2 mumol/l) and the urine ascorbate excretion 1531 (391-2934) mumol/g creatine. No significant differences could be observed between the various sport groups, or between the sport groups and controls with respect to absolute (mg/d) and relative (mg/g body weight) AA-intake, serum and urine concentrations. Only a few of the athletes had AA-intake below the RDA or serum- or urine levels smaller than the decision limit. The absolute AA-intake (n = 44) from the 7-day record (r = 0.49, p < 0.0009) and the AA-intake on the last day (1-day) prior to urine collection (r = 0.90, p < 0.0000) correlate moderately/strongly with the urinary excretion. Between AA-intake (7-day) and serum concentration there is a correlation of r = 0.59, p < 0.0000. The AA-status of highly trained athletes does not differ significantly from the control group in spite of intensive daily training. Thus, AA-supplementation beyond the normal daily intake does not appear necessary.