Using Intent-to-Attend to Predict Attendance in Community-Based Relationship Education.

Government-supported relationship education has provided resources for inclusion of economically vulnerable and ethnically diverse participants; however, many grantees and programs struggled to retain couples in longitudinal studies, which has likely influenced study effects and threatened internal validity. In the present study, we assessed 1,056 couples' baseline relationship satisfaction and intent-to-attend their next scheduled visit while participating in a randomized controlled trial of relationship education and evaluated the predictive ability of their responses to remain in the six-month study. We conducted actor-partner interdependence models for couples, using a probit cross-lagged regression with a structural equation modeling framework, to test the dyadic influence of intent-to-attend on future couple attendance. We also examined the influence of higher or lower baseline relationship satisfaction between partners and group assignment (treatment or wait-list control) on attendance. Intent-to-attend scores were associated with attendance for couples at the one-month follow-up, and early attendance was the biggest predictor of later attendance. Additionally, baseline intent-to-attend scores predicted later intent-to-attend scores for all follow-up time points. However, we found no partner effects, and no effects for the influence of baseline relationship satisfaction or group assignment. We discuss practical suggestions for including intent-to-attend in future studies, relationship education programming, and general therapy practice.

La capacitación en relaciones financiada por el gobierno ha facilitado recursos para la inclusión de participantes económicamente vulnerables y de distintas etnias; sin embargo, a muchos beneficiarios y programas les costó mantener a las parejas en los estudios longitudinales, lo cual probablemente haya influido en los efectos de los estudios y amenazado su validez interna. En el presente estudio, evaluamos la satisfacción con la relación en el momento basal de 1056 parejas y la intención de asistir a su próxima visita programada mientras participaban en un ensayo controlado aleatorizado de capacitación en relaciones, y evaluamos la capacidad predictiva de sus respuestas para permanecer en el estudio de seis meses. Implementamos modelos de interdependencia actor-pareja para las parejas usando un modelo Probit de regresión y retardo cruzado con un marco de modelos de ecuaciones estructurales con el fin de evaluar la influencia diádica de la intención de asistir en la asistencia futura de la pareja. También analizamos la influencia del nivel más bajo o más alto de satisfacción con la relación en el momento basal entre los integrantes de la pareja y la distribución a un grupo (de tratamiento o de control en lista de espera) en la asistencia. Los puntajes de la intención de asistir estuvieron asociados con la asistencia de las parejas en el seguimiento de un mes, y la asistencia inicial fue la mayor predictora de la asistencia posterior. Además, los puntajes de la intención de asistir en el momento basal predijeron los puntajes posteriores de la intención de asistir de todos los momentos de seguimiento. Sin embargo, no hallamos efectos de la pareja ni efectos de la influencia de la satisfacción con la relación o la distribución a un grupo en el momento basal. Comentamos sugerencias prácticas para incluir la intención de asistir en estudios futuros, en programas de capacitación en relaciones y en la práctica de la terapia general.

The Dyadic Association among Technoference and Relationship and Sexual Satisfaction of Young Adult Couples.

Technology use within relationships has been shown to produce potential rewards and costs that influence relationship development and/or sustainment; however, the negative influence of modern technology (i.e., smartphone, computer, tablet, and/or television) use in romantic relationships during quality time spent together (i.e., technoference) remains an understudied area. Even less is known about the effects of technoference on young adult couples' relationship satisfaction and sexual satisfaction. The current study utilized a descriptive correlational survey design with 158 young adult couples. We used actor-partner interdependence modeling to test the dyadic associations between technoference and relationship and sexual satisfaction among the young adult couple sample. Results indicated statistically significant negative actor effects between technoference and relationship and sexual satisfaction and statistically significant negative partner effects between technoference and relationship satisfaction.

The Relationship Between Social Support and Family Relationships Among Low-Income Couples Attending Relationship Education.

Results are mixed for relationship education (RE) interventions with low-income couples. For couples who experienced positive changes, it is not clear what aspects of program models contributed to change. Many low-income couples attend government-funded RE with limited access to social and community resources. Program models often provide related resources complimentary to RE skill-building. We examined the relationship between income, social support, and family functioning for low-income, ethnically diverse couples (N = 856) who attended RE, as well as the mediating effects of social support on family functioning outcomes. Analyses included three separate dyadic models that examined associations among constructs at baseline and immediately following the RE intervention. Results demonstrated relationships between participants' reported social support and family functioning such that (a) social support was associated with baseline family functioning for both men and women; (b) men's baseline social support was influenced by women's baseline family functioning; and (c) men's and women's social support change score had a positive influence on their own family functioning change score. However, social support was not a significant mediator of change in family functioning. Implications for RE practice and research are also discussed.

Los resultados de las intervenciones sobre educación para las relaciones (ER) con parejas de bajos recursos son variados. En el caso de las parejas que tuvieron cambios positivos, no está claro qué aspectos de los modelos del programa contribuyeron al cambio. Muchas parejas de bajos recursos asisten a ER financiadas por el gobierno con acceso limitado a recursos sociales y comunitarios. Los modelos del programa generalmente proporcionan recursos relacionados complementarios al desarrollo de habilidades para la ER. Analizamos la relación entre los ingresos, el apoyo social y el funcionamiento familiar en las parejas de bajos recursos y étnicamente diversas (N = 856) que asistieron a la ER, así como los efectos mediadores del apoyo social en los resultados del funcionamiento familiar. Los análisis incluyeron tres modelos diádicos independientes que examinaron las asociaciones entre los constructos al inicio del estudio e inmediatamente después de la intervención sobre ER. Los resultados demostraron las relaciones entre el apoyo social informado por los participantes y el funcionamiento familiar, por lo que (a) el apoyo social estuvo asociado con el funcionamiento familiar al inicio del estudio tanto en los hombres como en las mujeres; (b) el apoyo social de los hombres al inicio del estudio estuvo influenciado por el funcionamiento familiar de las mujeres al inicio del estudio; y (c) el puntaje de cambio del apoyo social de los hombres y las mujeres tuvo una influencia positiva en su propio puntaje de cambio del funcionamiento familiar. Sin embargo, el apoyo social no fue un mediador de cambio significativo en el funcionamiento familiar. También se debaten las implicancias para la investigación y la práctica en RE.

Pooled peripheral blood mononuclear cells provide an optimized cellular substrate for human immunodeficiency virus Type 1 isolation during acute infection.

BACKGROUND: Cellular substrate (CS) composed of phytohemagglutinin (PHA)-stimulated PBMNCs is required for isolating primary human immunodeficiency virus Type 1 from plasma (PHIV) during early acute infection. The transmitted founder PHIV is neutralization-sensitive and uses chemokine-receptor 5 to infect CD4-positive PBMNCs. Therefore, PHIV cocultured with optimized CS would enable efficient virologic diagnosis, vaccine development, and tests for broadly neutralizing antibodies (bnAb). STUDY DESIGN AND METHODS: Fifteen leukapheresis donations were used to isolate CD4-enriched PBMNCs. Individual or three or four donors' pooled cells were stimulated with PHA, and both CSs were evaluated in parallel cocultures using five different PHIV isolates from nucleic acid testing-positive anti-HIV-negative donations. Feasibility of coculturing PHIV with pooled PBMNC-CS after dimethyl sulfoxide cryopreservation was investigated. RESULTS: The cell-free supernatants from individual CSs contained mean HIV-p24 antigen varying between 20.35 and 85.54 ng/mL, while that from pooled CSs ranged between 57.54 and 88.10 ng/mL. Thus, PBMNCs pooled from multiple donors provide an optimal CS for coculturing PHIV. Cryopreserved PBMNC-CS for PHIV cocultures is promising for systematic biosynthesis of PHIV. CONCLUSIONS: Pooled CSs after cryopreservation were functional for PHIV replication and enable on-demand diagnostic cocultures, preparing panels of PHIV stocks for bnAb testing and developing an envelope-subunit vaccine candidate analogous to hepatitis B surface antigen, proven successful in preventing hepatitis B virus infection.