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Biosci Biotechnol Biochem ; 83(2): 251-261, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30317922

RESUMO

Cucurbitaceae plants contain characteristic triterpenoids. Momordica charantia, known as a bitter melon, contains cucurbitacins and multiflorane type triterpenes, which confer bitter tasting and exhibit pharmacological activities. Their carbon skeletons are biosynthesized from 2,3-oxidosqualene by responsible oxidosqualene cyclase (OSC). In order to identify OSCs in M. charantia, RNA-seq analysis was carried out from ten different tissues. The functional analysis of the resulting four OSC genes revealed that they were cucurbitadienol synthase (McCBS), isomultiflorenol synthase (McIMS), ß-amyrin synthase (McBAS) and cycloartenol synthase (McCAS), respectively. Their distinct expression patterns based on RPKM values and quantitative RT-PCR suggested how the characteristic triterpenoids were biosynthesized in each tissue. Although cucurbitacins were finally accumulated in fruits, McCBS showed highest expression in leaves indicating that the early step of cucurbitacins biosynthesis takes place in leaves, but not in fruits. Abbreviations: OSC: oxidosqualene cyclase; RPKM: reads perkilobase of exon per million mapped reads.


Assuntos
Genes de Plantas , Transferases Intramoleculares/genética , Momordica charantia/genética , Análise de Sequência de RNA/métodos , Triterpenos/metabolismo , Sequência de Aminoácidos , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Transferases Intramoleculares/química , Transferases Intramoleculares/metabolismo , Momordica charantia/enzimologia , Reprodutibilidade dos Testes , Saccharomyces cerevisiae/genética , Homologia de Sequência de Aminoácidos
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