The retinoblastoma tumor suppressor protein targets distinct general transcription factors to regulate RNA polymerase III gene expression.

The retinoblastoma protein (RB) represses RNA polymerase III transcription effectively both in vivo and in vitro. Here we demonstrate that the general transcription factors snRNA-activating protein complex (SNAP(c)) and TATA binding protein (TBP) are important for RB repression of human U6 snRNA gene transcription by RNA polymerase III. RB is associated with SNAP(c) as detected by both coimmunoprecipitation of endogenous RB with SNAP(c) and cofractionation of RB and SNAP(c) during chromatographic purification. RB also interacts with two SNAP(c) subunits, SNAP43 and SNAP50. TBP or a combination of TBP and SNAP(c) restores efficient U6 transcription from RB-treated extracts, indicating that TBP is also involved in RB regulation. In contrast, the TBP-containing complex TFIIIB restores adenovirus VAI but not human U6 transcription in RB-treated extracts, suggesting that TFIIIB is important for RB regulation of tRNA-like genes. These results suggest that different classes of RNA polymerase III-transcribed genes have distinct general transcription factor requirements for repression by RB.

[Episiotomy and its complications]. / Episiotomie und ihre Komplikationen.

1. Many benefits claimed for episiotomy are not sufficiently proven. In recent literature, some of them are questioned and some have been disproven. 2. Episiotomy, especially median episiotomy, has a higher risk of third-degree lacerations. Mediolateral episiotomy is more often followed by postpartum pain and impaired wound-healing. 3. Typical, albeit rare complications of episiotomy and third-degree lacerations are incontinence for stool and flatus, and-very seldom-fistula formation. 4. Complications of episiotomy as well as the failure to perform an episiotomy have had forensic consequences. 5. For good healing of an episiotomy or a perineal laceration suturing with an adequate technique and the use of non-reactive suture material is mandatory.

[Pelvic floor reconstruction after traumatic childbirth damage]. / Beckenbondenrekonstruktion nach geburtstraumatischen Schäden.

Vaginal delivery can result in direct injury to the anterior part of the anal sphincter. The goal of its repair is to restore the separated sphincters and connective tissue to their anatomical positions in order to achieve continence. The results are best when an anal sphincter rupture is repaired immediately following delivery, when the vascularity of the perineum and perivaginal tissues favours healing.

[Perforation of surgical gloves in gynecologic operations and abdominal Cesarean section]. / Handschuhperforationen bei gynäkologischen Operationen und abdominalen Schnittentbindungen.

The aim of the study was to determine the incidence of glove perforation during gynaecological operations and Caesarean section, and to assess the value of double gloving. For this purpose the surgical gloves used in 415 procedures were tested for perforations by the water leak test. For laparotomy and breast surgery, two pairs of gloves (brand A) were worn; for vaginal and other surgery, only a single pair of thicker gloves (brand B) was used. As controls, 75 pairs of unused gloves of each brand were tested. This revealed 6 (4%) perforations in brand A gloves and 2 (1.3%) perforations in brand B gloves. Most perforations (20-40%) occurred during hysterectomy, Caesarean section, and other types of laparotomy. The gloves worn by the scrub nurse or technician were most often perforated (43.5% after vaginal hysterectomy), followed by the surgeon's gloves (29% after laparotomy). Perforations were most often located at the tip of the index finger (16.8%) and thumb (16.2%) of the nondominant hand. If two pairs of gloves were worn, and perforation occurred, only 26.7% had perforations at identical sites on the outer and inner gloves. Consequently, since three quarters of the perforations were limited to the outer glove, double gloving reduced the risk of exposure to blood by a factor of 4. The results of this study support the recommendation that two pairs of gloves be worn, at least during major surgical procedures and Caesarean section. Furthermore, the alternative operative techniques and methods of handling surgical instruments proposed for reducing the incidence of glove perforation should be tested.

Delivery of very premature infants: does the caesarean section rate relate to mortality, morbidity, or long-term outcome?

A retrospective analysis of obstetric factors influencing mortality and morbidity of very premature infants (1500 g, less than or equal to 32 weeks' gestation) was undertaken. The study included 275 such infants born in the Department of Obstetrics of the University of Tübingen during the period January 1977 to June 1987. The caesarean section rate of very preterm infants increased from 28% during the period 1977-1982 to 87% during the period 1982-1987 (P less than 0.005), accompanied by an increase in survival rate from 63% to 70%. The improvement in survival rate was statistically significant for the group with birth weight 751-1000 g (P less than 0.01). The overall mortality rate was 31% after caesarean section and 36% after vaginal delivery. Amongst the causes of death of the non-survivors, acidosis was more frequent and amniotic infection syndrome less frequent in the infants delivered vaginally than in those delivered abdominally. The proportion of children with normal development at two years of age was significantly (P less than 0.02) greater amongst those born in 1982-1987 than in those born in 1977-1981. The interpretation of these findings is by no means clear but must include the hypothesis that the increased caesarean section rate may be incidental and in no way related to the improved outcome.

[Prevention of vaginal prolapse in hysterectomy by suspension of the vaginal stump]. / Prophylaxe des Scheidenvorfalls bei der Hysterektomie durch Suspension des Vaginalstumpfes.

For prophylaxis of enterocele and of prolapse of the vagina following hysterectomy, the vaginal stump is fixed in at-risk patients to the sacro-uterine ligaments (known as McCall's suture) or to the sacro-spinal ligament (Amreich-Richter method). We report on the indications and results obtained in 101 sacro-spinal fixations and 211 McCall sutures in vaginal hysterectomy and 118 McCall sutures in abdominal hysterectomy. From 1975 to 1981 sacro-spinal fixation was only occasionally employed in prophylaxis of enterocele. After introduction of the McCall suture in 1982, the use of this method has been steadily increasing and has largely replaced sacrospinal fixation for prophylactic purposes. Nevertheless we are still using this often in cases of total prolapse, since in that situation, the fixation of the vaginal stump to the sacrouterine ligaments (in most cases weakly developed) is insufficient and does not offer enough support. Of a total of 350 McCall sutures performed to date, postrenal anuria occurred twice after kinking of the ureters, a typical complication that requires removal of the McCall suture. In a total of 174 sacro-spinal fixations of the vaginal stump for prophylactic or therapeutic indications, pronounced intraoperative haemorrhage took place in about 5% of the cases, whereas in one case, there was an abscess formation due to an infected haematoma. Technical details on both methods and on avoiding complications are discussed.

[Prevention of HIV infections in surgical gynecology and obstetrics]. / Schutz vor HIV-Infektionen in der operativen Gynäkologie und Geburtshilfe.

In professionally conditioned HIV infections of medical personnel, blood has so far been the only source of infection that is of any importance. Hence, measures to prevent such infections must primarily aim at avoiding any contact with blood from HIV-infected patients. Since the HIV status of the patients and possibly alos other infections transmitted by blood (hepatitis) are often unknown, the following protective measures must be applied in all patients as a matter of routine. Fundamentally, gloves must always be worn when coming into contact with blood, body fluids, mucosa and non-intact skin. In operative procedures and deliveries, barrier methods must be employed to protect the eyes, nose, mouth and skin, as well as technical procedures during surgery and organisational measures to prevent any possible injuries or lesions caused by operative interventions and to reduce the number of persons exposed to risk. So far, needle pricks have been the most frequent mode of infection. Hence, used cannules should be treated with utmost care; do not return them into their protective cover but throw them directly into solid waste containers. After contamination, blood and other body fluids should be eliminated immediately. In case of massive contamination with material containing HIV, chemoprophylaxis should be given careful consideration.

[Fertility following tubal pregnancy: comparison of tube-saving surgery and salpingectomy]. / Fertilität nach Tubargravidität: Vergleich von tubenerhaltenden Operationen und Salpingektomien.

391 patients were operated during 1980 to 1987 at the Department of Gynaecology of the University of Tübingen for extrauterine pregnancy. A questionnaire was circulated to inquire, how many of these women wanted to become pregnant again. 176 answered positively, the return quota being 82%. After surgery, performed to preserve the Fallopian tubes, 64% of these patients had an intrauterine pregnancy, compared with only 41% after salpingectomy or segmental resection without anastomosis. In women without the characteristic factors which reduce fertility, such as primary sterility, surgery concerning sterility or refertilization, or other kinds of abdominal surgery, a greater proportion of intrauterine pregnancies was seen after surgery preserving the Fallopian tubes (79%), than in women with the above mentioned risk factors (53%). The incidence of repeat extrauterine pregnancies was approximately equal after Fallopian tube-preserving surgery and after salpingectomy (20% and 18%, respectively).

[Late complications of episiotomy]. / Spätkomplikationen nach Episiotomie.

By means of a questionnaire and partly by phone calls 467 women who delivered at the Universitäts-Frauenklinik Tübingen 1-5 years ago were interviewed regarding perineal discomfort, wound healing and anal incontinence. 106 women have had a mediolateral episiotomy, 250 a median episiotomy (including 115 complete perineotomies) and 111 women have had no episiotomy. Dyspareunia was reported in 47% of the primiparous woman and by 22% of multiparous women with episiotomy; by contrast without episiotomy, this problem occurred only in 7% and 8% of primiparous and multiparous patients respectively (p less than 0.01). Perineal pain while sitting on a chair was reported by 30% of women with mediolateral episiotomy as compared to 19% of those with median episiotomy or complete perineotomy and 4.5% without episiotomy (median episiotomy vs. mediolateral episiotomy: p less than 0.05, episiotomy vs. no episiotomy: p less than 0.001). Occasional involuntary passage of flatus occurred in 18%, 22% and 14% respectively (differences not significant); involuntary passage of faeces in 7%, 9% and 1% respectively (episiotomy vs. no episiotomy: p less than 0.01). The occasional involuntary passage of faeces persisted for more than 6 months in 2 of 106 women with mediolateral episiotomy and in 3 of 250 women with median episiotomy. Because of the good healing of adequately reconstructed perineal tears and the better outcome of median episiotomy as compared to mediolateral episiotomy the authors advocate to use episiotomy restrictively and, if enlargement of the vaginal outlet is indicated, to use median rather than mediolateral episiotomy. If necessary, median episiotomy may be extended to complete perineotomy.

Clinical evaluation of terconazole. European experience.

Terconazole is a new topical antifungal agent that differs structurally and functionally from the imidazoles. European clinical trials were conducted to determine (1) the lowest effective dose for a given treatment period, (2) which formulation should be tested further, and (3) how terconazole compares with other topical antifungal agents in terms of safety and efficacy. The results of dose-response studies demonstrated that 80- and 240-mg suppositories and 0.4% cream were the most effective formulations. Data from multicenter studies of pregnant and nonpregnant women in Belgium and Luxembourg indicate that the efficacy of terconazole cream is superior to that of miconazole nitrate cream and clotrimazole cream. Terconazole cream is also more effective than clotrimazole cream in terms of lower relapse rates.

[Comparison of the results of preoperative studies with imaging procedures with the surgical status of ovarian cancer]. / Vergleich der präoperativen Untersuchungsergebnisse der bildgebenden Verfahren mit dem operativen Situs beim Ovarialkarzinom.

Optimal cytoreductive surgery of ovarian cancer is based on the preoperative diagnosis and assessment of tumour spread. Of 147 patients who underwent staging laparotomy at the Department of Gynaecology of the University of Tübingen, intraoperative staging was compared retrospectively with the results of sonography, computed tomography, double-contrast enema and urography. Ultrasound and computed tomography were comparable concerning accuracy of the diagnosis in 86 and 79% of the cases, respectively. Combined application of both methods resulted in an accuracy of 90%. Involvement of colon was diagnosed by double-contrast enema in only 41% of the cases in which enterotomy had to be performed. Involvement of bladder and ureter was observed in 80% of the cases by intravenous urography. According to our results abdominal ultrasound and urography should be performed in patients with palpable pelvic masses. The application of computed tomography as an additional method is indicated in patients with tumour classified as benign by sonographic examination. Double-contrast enema is of limited value in the diagnosis of colon involvement.

[Results of organ-saving therapy in tubal pregnancy]. / Ergebnisse der organerhaltenden Therapie der Eileiterschwangerschaft.

Worldwide, the incidence of nonruptured tubal pregnancy has increased, and so has the feasibility of conservative management of this condition. Following conservative surgery the rate of intrauterine pregnancy is significantly higher than after salpingectomy. The rate of ectopic pregnancy has not (or hardly) increased. For a surgeon skilled in this technique, the laparoscopic approach has advantages because it avoids laparotomy. For the time being, medical treatment of ectopic pregnancy with methotrexate, prostaglandins, and antiprogesterone should be confined to clinical studies. For nonviable, nonruptured tubal pregnancy with decreasing HCG titers expectant management seems possible; following conservative treatment, monitoring of HCG until it becomes undetectable is mandatory.

Effect of cesarean section on outcome in high- and low-risk very preterm infants.

The effect of mode of delivery on the survival and morbidity of 24- to 32-week infants (500-1500 g) was studied in 262 consecutive deliveries. The study population was divided into high-risk (e.g., hypertension) and low-risk (e.g., incompetence of the cervix) groups by evaluation of risk factors. 194 very preterm newborn were classified as high risk and 68 as low risk. In both groups the perinatal outcome of vaginal delivery and cesarean section delivery was compared. Cesarean section was associated with a highly significantly improved survival rate in the high-risk group, but was not associated with differences in fetal outcome in the low-risk group. The results of this study do not support primary cesarean section as the method of delivery for all very preterm fetuses.

[Incidence and significance of pathogen detection in Douglas fluid in non-inflammatory genital diseases]. / Häufigkeit und Bedeutung des Keimnachweises in der Douglasflüssigkeit bei nichtentzündlichen Genitalerkrankungen.

Between 1976 and 1986 the secretions from the pouch of Douglas of 1,219 patients with non-inflammatory genital diseases were microbiologically studied. Microorganisms were detected in 3.4%. In the majority of cases they were physiological skin organisms, with a count of less than 10 per ml of Douglas fluid. These findings support the view, that secondary contamination of the specimens occurred during removal, transportation, or laboratory processing.

[Laparoscopic findings in suspected adnexitis]. / Laparoskopische Befunde bei Verdacht auf Adnexitis.

Between 1976 and 1985 laparoscopy was performed on a total of 768 patients in whom adnexitis was suspected. In accordance with conventional usage they were initially designated as "acute" and "chronic" cases of adnexitis on the basis of clinical criteria. The tentative diagnosis of an active infection was confirmed by laparoscopy in 62.7% of the patients with the clinically acute form and 14.5% of those with "chronic" adnexitis. Although high temperature, leukocytosis, and an increased ESR were more common among patients with laparoscopically confirmed adnexitis, high levels of inflammatory reaction were also found in patients with other conditions. In 164 patients (21%) no pathological findings were found in the genital and abdominal regions. The results confirm the importance of laparoscopy in the diagnosis of adnexitis.