RESUMO
The complete nucleotide sequence of human papillomavirus type 41 (HPV-41) has been determined. HPV-41 was originally isolated from a facial wart, but its DNA has subsequently been detected in some skin carcinomas and premalignant keratoses (Grimmel et al., Int. J. Cancer, 1988, 41, 5-9; de Villiers, Grimmel and Neumann, unpublished results). The analysis of the cloned HPV-41 nucleic acid reveals that its genome organisation is characteristic as for other papillomavirus types. Yet, the analysis indicates at the same time that this virus is most distantly related to all other types of human-pathogenic papillomaviruses sequenced thus far and appears to identify HPV-41 as the first member of a new subgroup of HPV. The overall nucleotide homology to other sequenced HPV types is below 50%. The closest other HPV type is represented by HPV-18, sharing 49% identical nucleotides. The typical E2 binding sequence ACCN6GGT, found in all papillomaviruses analyzed to date, does not occur in the URR of the HPV-41 genome. Modified E2 binding sequences, as described for BPV 1 (Li et al., Genes Dev. 1989, 3, 510-526), are located in the domain proximal to the E6 ORF. These are ACCN6GTT, AACN6GGT and the two perfect palindromic sequences AACGAATTCGTT.
Assuntos
Genes Virais , Papillomaviridae/genética , Sequência de Aminoácidos , Sequência de Bases , Sítios de Ligação , Clonagem Molecular , Códon/genética , Humanos , Dados de Sequência Molecular , Fases de Leitura Aberta , Regiões Promotoras Genéticas , Sequências Repetitivas de Ácido Nucleico/genética , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica , Proteínas Virais/genéticaRESUMO
HPV 2a is commonly associated with verrucae vulgares, whereas HPV 57 was detected in mucosal lesions of the maxillary sinus and the genital tract, as well as in cutaneous lesions. The complete DNA sequences of HPV 2a and HPV 57 were determined. The HPV 2a genome consists of 7860 base pairs and the HPV 57 genome contains 7861 base pairs. On the nucleotide level an 83% homology between the two sequences could be ascertained. Compared to other HPVs they have a high G/C-content (HPV 2a: 48.8%, HPV 57: 50.1%). The genomic organization of both viruses complies with that of other sequenced HPVs. Significant sequence divergence between the HPV 2a and HPV 57 genomes was found in the long control region (LCR), as well as in the early-late-region (ELR). The latter varies in size between the cutaneous (72 to 103 nucleotides) and the mucosal HPVs (252 to 584 nucleotides). According to the sizes of the ELRs of HPV 2a (377 nucleotides) and HPV 57 (478 nucleotides), as well as DNA sequence comparisons, these two viruses could be grouped with the so-called mucosal HPVs. In a search for possible tissue-specific elements, a common amino acid motif, thr-thr/asp-pro-ala-ile/valile/leu was found in the L2 of all mucosal HPVs, as well as in HPV 2a and 57. The L2 of the cutaneous types contain the motif val-ser/thr-arg-thr-gln-tyr.
Assuntos
DNA Viral/química , Papillomaviridae/genética , Homologia de Sequência do Ácido Nucleico , Sequência de Aminoácidos , Composição de Bases , Sequência de Bases , Humanos , Dados de Sequência Molecular , Fases de Leitura AbertaRESUMO
Two new papillomaviruses, HPV 40 and HPV 57, were isolated from a PIN lesion and an inverted papilloma of the maxillary sinus, respectively. HPV 40 showed a 13% homology to HPV 7 by reassociation kinetics and HPV 57 showed a 17% homology to HPV 2 and 25% homology to HPV 27. Hybridization of the DNA of these papillomaviruses to a wide variety of different tumor biopsies revealed that HPV 40 was present in a few genital condylomata acuminata as well as in bowenoid lesions. HPV 57 DNA was present in an oral wart, a genital condyloma acuminatum, and verrucae vulgares lesions from two immunosuppressed patients.
