RESUMO
Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.
Assuntos
Anticorpos/imunologia , Ácidos Graxos/química , Glicoproteínas/isolamento & purificação , Proteínas de Insetos/isolamento & purificação , Animais , Anexina A2/química , Cromatografia Líquida de Alta Pressão , Ensaio de Imunoadsorção Enzimática , Ácidos Graxos/metabolismo , Glicoproteínas/química , Glicoproteínas/imunologia , Proteínas de Insetos/química , Proteínas de Insetos/imunologiaRESUMO
To prove the pharmacological actions of honeybee royal jelly (RJ) on the nervous system, we examined the effects of RJ on CRE-mediated transcription. RJ increased CRE-mediated transcription in PC12D cells. Moreover, CRE-mediated transcriptional activity by RJ was enhanced by nobiletin. U0126, a MEK inhibitor, inhibited CRE-mediated transcription by combining RJ plus nobiletin without affecting transcription by RJ alone. These results suggest that RJ stimulates CRE-mediated transcription via an ERK-independent cascade, whereas the increasing CRE-mediated transcriptional effect by nobiletin is dependent on ERK phosphorylation. Combining RJ plus nobiletin may activate effectively neuronal functions via enhancement of CRE-mediated transcription.
