RESUMO
Functionally asymmetric somatic hybrids possessing heterologous transposable element Spm/dSpm were obtained following intertribal somatic hybridization between Brassica napus and transgenic Arabidopsis thaliana. Mobile genetic elements actively transposed in the hybrid genomes. Complete elimination of A. thaliana genome was not observed.
Assuntos
Arabidopsis/genética , Brassica napus/genética , Elementos de DNA Transponíveis/genética , DNA de Plantas/genética , Hibridização Genética/genética , Zea mays/genética , Genes de Plantas , Plantas Geneticamente Modificadas , Transcrição GênicaRESUMO
Intertribal somatic hybridization between wild type Brassica juncea (L.) Czern. & Coss. and transgenic A. thaliana L. has been carried out. Genome of A. thaliana plants contained heterologous transposable element Spm/dSpm, reporter GUS gene, selective genes for kanamycin- (npt II) and phosphinothricin (bar) resistance. Hybrid nature of obtained plants was confirmed with their morphology, GUS hystochemical assay, PCR-RFLP, RAPD and isozyme analyses. It was determined that heterologous transposable element Spm/dSpm is able to function in hybrid plants. There was no complete elimination of A. thaliana genetic material in the hybrids and the transgenes were stably maintained.
Assuntos
Arabidopsis/genética , Elementos de DNA Transponíveis/genética , DNA de Plantas/genética , Hibridização Genética/genética , Mostardeira/genética , Plantas Geneticamente Modificadas , Amilases/metabolismo , Arabidopsis/enzimologia , Arabidopsis/crescimento & desenvolvimento , Primers do DNA , Eletroforese em Gel de Ágar , Esterases/metabolismo , Genes de Plantas/genética , Genes Reporter/genética , Vetores Genéticos , Mostardeira/enzimologia , Mostardeira/crescimento & desenvolvimento , Brotos de Planta/enzimologia , Brotos de Planta/genética , Brotos de Planta/crescimento & desenvolvimento , Protoplastos/enzimologia , Seleção GenéticaRESUMO
A method of Agrobacterium-mediated genetic transformation of sugarbeet (Beta vulgaris L.) by vacuum infiltration has been developed. Transgenic sugarbeet plants of Ukraine breeding were selected for their resistance either to the antibiotic kanamycin or to the herbicide glufosinate ammonium. Integration of transgenes was confirmed by PCR and GUS-assay.
