Metrics for Success: Strategies for Enabling Core Facility Performance and Assessing Outcomes.

Core Facilities are key elements in the research portfolio of academic and private research institutions. Administrators overseeing core facilities (core administrators) require assessment tools for evaluating the need and effectiveness of these facilities at their institutions. This article discusses ways to promote best practices in core facilities as well as ways to evaluate their performance across 8 of the following categories: general management, research and technical staff, financial management, customer base and satisfaction, resource management, communications, institutional impact, and strategic planning. For each category, we provide lessons learned that we believe contribute to the effective and efficient overall management of core facilities. If done well, we believe that encouraging best practices and evaluating performance in core facilities will demonstrate and reinforce the importance of core facilities in the research and educational mission of institutions. It will also increase job satisfaction of those working in core facilities and improve the likelihood of sustainability of both facilities and personnel.

Subventricular zone cell migration: lessons from quantitative two-photon microscopy.

Neuroblasts born in the adult subventricular zone (SVZ) migrate long distances in the rostral migratory stream (RMS) to the olfactory bulbs where they integrate into circuitry as functional interneurons. As very little was known about the dynamic parameters of SVZ neuroblast migration, we used two-photon time-lapse microscopy to analyze migration in acute slices. This involved analyzing 3D stacks of images over time and uncovered several novel aspects of SVZ migration: chains remain stable, cells can be immotile for extensive periods, morphology does not necessarily correlate with motility, neuroblasts exhibit local exploratory motility, dorsoventral migration occurs throughout the striatal SVZ, and neuroblasts turn at distinctive angles. We investigated these novel findings in the SVZ and RMS from the population to the single cell level. In this review we also discuss some technical considerations when setting up a two-photon microscope imaging system. Throughout the review we identify several unsolved questions about SVZ neuroblast migration that might be addressed with current or emerging techniques.

MEF-2 regulates activity-dependent spine loss in striatopallidal medium spiny neurons.

Striatal dopamine depletion profoundly reduces the density of spines and corticostriatal glutamatergic synapses formed on D(2) dopamine receptor expressing striatopallidal medium spiny neurons, leaving D(1) receptor expressing striatonigral medium spiny neurons relatively intact. Because D(2) dopamine receptors diminish the excitability of striatopallidal MSNs, the pruning of synapses could be a form of homeostatic plasticity aimed at restoring activity into a preferred range. To characterize the homeostatic mechanisms controlling synapse density in striatal medium spiny neurons, striatum from transgenic mice expressing a D(2) receptor reporter construct was co-cultured with wild-type cerebral cortex. Sustained depolarization of these co-cultures induced a profound pruning of glutamatergic synapses and spines in striatopallidal medium spiny neurons. This pruning was dependent upon Ca(2+) entry through Cav1.2 L-type Ca(2+) channels, activation of the Ca(2+)-dependent protein phosphatase calcineurin and up-regulation of myocyte enhancer factor 2 (MEF2) transcriptional activity. Depolarization and MEF2 up-regulation increased the expression of two genes linked to synaptic remodeling-Nur77 and Arc. Taken together, these studies establish a translational framework within which striatal adaptations linked to the symptoms of Parkinson's disease can be explored.

A Rationale and Outline for an Undergraduate Course on the Philosophy and History of Science for Life Science Students.

There are compelling reasons for teaching a philosophy of science course to undergraduate life science students. The main reason is to help them understand that modern science is not based upon a single, consistent philosophical system; nor is it based upon common sense, or a method, set of rules or formulas that can be used to make unerring predictions. Rather, science is a dynamic process that is constantly being modified and refined to reflect and encompass an ever-expanding set of hypotheses, observations, and theories. To illustrate these points, we developed a course that examined the history and philosophical underpinnings of modern science, and we discussed famous experiments that challenged the prevailing norm and led to Kuhnian revolutions in scientific thought. Building upon this knowledge, students investigated how different philosophical systems address controversial social issues in the biological sciences. They examined the teaching of intelligent design and creationism in public schools, the implications of legalized abortion and physician-assisted suicide, the potential impact of DNA fingerprinting on human rights and racism, the promise and pitfalls of stem cell research, and the neurobiological basis of consciousness and its relevance to mental health therapies and the animal rights movement. We believe undergraduate life science students should be exposed to these issues and have an opportunity to develop informed opinions about them before they graduate from college. Exploration of such topics will help them become better prepared for the inevitable public debates that they will face as science educators, researchers, and leaders of society.