RESUMO
The fine structure of the normal mucous membrane in the human maxillary sinus was investigated by means of the freeze-fracture technique. Special interest was directed to membrane structures in the epithelial cells. The morphology of tight junctions could be analysed. According to morphological criteria these junctions might be classified as "very tight". So called ciliary necklaces were well distinguishable. Their morphology seemed to be in concordance with the structure reported in other mammalian respiratory epithelia. Abluminally the epithelial cells frequently displayed abundant caveolae or micropinocytotic vesicles. The present investigation was performed as a preface to later studies on pathologically altered antral mucosa.
Assuntos
Mucosa Gástrica/ultraestrutura , Antro Pilórico/ultraestrutura , Cílios/ultraestrutura , Técnica de Fratura por Congelamento , Mucosa Gástrica/citologia , Humanos , Junções Intercelulares/ultraestrutura , Microscopia Eletrônica , PinocitoseRESUMO
A modification of the conventional method of freeze-fracturing is described. By the use of polyvinyl-alcohol, a high viscous fluid, the organ of Corti is better stabilized in the specimen holder. This fluid also facilitates a clean fracture through the organ of Corti proper and in a high number of cases gives a large replica void of contaminating biological tissue and free of folds. Examples of results produced with this technique are demonstrated.
