RESUMO
Two novel HLA class II alleles have been identified in routine typing of a kidney transplant patient and a cord blood unit from the Australian Cord Blood Bank in Sydney. Sequence analysis of exon 2 of the DQB1 genes revealed the novel polymorphism. A substitution of A to C at nucleotide position 136 has been identified for the DQB1*0311 allele when compared to the closest-matched allele, DQB1*030201. An identical substitution has also been identified for the DQB1*0620 allele when compared to the closest-matched allele, DQB1*0602. The substitution results in an amino acid change from methionine to leucine at position 46 implicating different specificity and affinity of antigen binding.
Assuntos
Alelos , Substituição de Aminoácidos/genética , Éxons/genética , Antígenos HLA/genética , Polimorfismo Conformacional de Fita Simples , Sequência de Bases , Primers do DNA , Variação Genética , Humanos , Dados de Sequência MolecularRESUMO
Anew human leukocyte antigen-B allele, B*1565, has been identified during routine typing of cord blood samples. Subsequently, two individuals from the same family as the first cord blood sample plus two unrelated Australian Bone Marrow Donor Registry samples have been found to carry this novel allele.
Assuntos
Antígenos HLA-B/genética , Sequência de Aminoácidos , Sequência de Bases , Éxons , Antígeno HLA-B15 , Humanos , Dados de Sequência MolecularRESUMO
Two novel human leukocyte antigen (HLA) class II alleles have been identified in routine typing of bone marrow donors for the Australian Bone Marrow Donor Registry in Sydney, Australia. Sequence analysis of exon 2 of both the DQB1 and DRB1 genes revealed the novel polymorphism. A silent substitution of G to A at nucleotide position 210 has been identified for the DQB1*030503 allele when compared to the closest matched allele, DQB1*030501. There is no associated amino acid difference between the translated products of the two alleles. The second new allele is a variant of the DRB1 gene. The DRB1*0447 allele was identified with three nucleotide substitutions compared to the closest matched allele DRB1*0436. There is a silent mutation at nucleotide position 303, G to C and two substitutions at adjacent nucleotide positions 344 and 345, T to G and G to T, respectively. The latter two substitutions result in an amino acid change from valine to glycine at position 86, implicating a different specificity and affinity of antigen binding.
Assuntos
Alelos , Antígenos HLA/genética , Teste de Histocompatibilidade , Polimorfismo Conformacional de Fita Simples , Austrália , Sequência de Bases , Transplante de Medula Óssea , Primers do DNA/genética , Éxons/genética , Antígenos HLA/imunologia , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Alinhamento de SequênciaRESUMO
We report the discovery of two HLA-DRB1 alleles by sequencing based typing (SBT). DRB1*04053 differs from previously reported DRB1 alleles by a single synonymous nucleotide substitution, resulting in a unique polymorphism at codon 93. DRB1*1143 differs from previously identified DRB1 alleles by a single non-synonymous nucleotide substitution, resulting in a polymorphism observed in other DRB1 and DRB3 alleles1.
Assuntos
Antígenos HLA-DR/genética , Alelos , Sequência de Bases , Cadeias HLA-DRB1 , Humanos , Dados de Sequência MolecularRESUMO
We report the discovery of four HLA-DRB1 alleles during routine sequencing based typing (SBT). These alleles--DRB1*03052, DRB1*04032, DRB1*1139 and DRB1*1346--differ from previously identified DRB1 alleles by known nucleotide polymorphisms.
Assuntos
Antígenos HLA-DR/genética , Alelos , Sequência de Bases , Sangue Fetal/imunologia , Antígenos HLA-DR/imunologia , Cadeias HLA-DRB1 , Teste de Histocompatibilidade/métodos , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
A new DRB1 allele has been identified during routine HLA-DRB1 generic typing with sequence specific oligonucleotides. The new allele was confirmed by PCR-restriction fragment length polymorphism analysis and subsequently sequenced. This showed the novel allele, named HLA-DRB1*1207, was similar in sequence to DRB1*12011, except for codon 69 of exon 2.
