RESUMO
A structured role-playing method of teaching the biopsychosocial interview using hidden agendas and videotape feedback was developed and evaluated. The teaching method significantly improved medical student interviewing skills as reflected by pre-post scores of 83 third-year students on the Facilitating Response Index. The teaching method is described in detail and recommendations for future research are presented.
Assuntos
Medicina de Família e Comunidade/educação , Entrevistas como Assunto , Desempenho de Papéis , Ensino/métodos , Educação Médica/normas , Educação Médica/tendências , Retroalimentação , Humanos , Entrevistas como Assunto/métodos , Relações Médico-Paciente , Gravação de VideoteipeRESUMO
The synthesis of H1 histones was studied in nine mouse-human somatic cell hybrid clones containing reduced numbers of human chromosomes. The entire human genome could be accounted for karyologically and by the use of functional assays for specific enzyme markers encoded by human chromosomes. Chromatographic resolution and peptide mapping of species-specific H1 histones failed to reveal human H1 histones to a level of about 1% of total in the nine clones. In addition to the species-specific extinction of human H1 histones, effects were seen on the quantity of mouse H1 histone subtypes produced in four of the nine clones. The remaining five clones produced H1 histones qualitatively and quantitatively identical with those of the mouse parent, which was common to all nine clones. The results suggest at least two levels of control for H1 histone gene expression.
Assuntos
Regulação da Expressão Gênica , Histonas/genética , Animais , Resinas de Troca de Cátion , Cromatografia por Troca Iônica , Bandeamento Cromossômico , Células Clonais , Fibroblastos , Histonas/biossíntese , Humanos , Células Híbridas , Cariotipagem , Camundongos , Resinas Sintéticas , Especificidade da EspécieRESUMO
Methodology is described to enrich for heterokaryons after mammalian cell fusion. A heterogeneous cell mixture can be separated on a Sta-Put apparatus into fractions of uniform size cells by sedimentation through a 1% bovine serum albumin-5% Ficoll gradient. Unfused RAG and LM/TK- cells, differing by 10% in diameter, have been sorted by size; following fusion, larger and faster sedimenting cells were shown to be hybrids. This methodology can be utilized in genetic complementation studies of human genetic diseases where selection procedures for proliferating hybrids do not exist. When fibroblasts from individuals with Tay-Sachs disease [deficient in hexosaminidase A (HEX A-)] and Sandhoff-Jatzkewitz disease (HEX A- and HEX B-) are fused, HEX A is generated, demonstrating complementation of two different mutations. After Sta-Put fractionation, the HEX A complementation product was associated with the faster sedimenting multinuclear cells and not with the mononuclear parental cells. This methodology will facilitate detection of genetic differences in fibroblasts from related inherited disorders.
Assuntos
Teste de Complementação Genética , Doenças Genéticas Inatas/classificação , Células Híbridas/metabolismo , Animais , Fusão Celular , Linhagem Celular , Separação Celular , Centrifugação com Gradiente de Concentração , DNA/metabolismo , Eletroforese em Gel de Amido , Humanos , Leucemia Experimental , Métodos , Camundongos , Doença de Sandhoff/genética , Doença de Tay-Sachs/genéticaRESUMO
A new technique of replica plating for CHO cells has been developed in which the pattern of colonies growing in a tissue culture plate is reproduced on a nylon cloth. The efficiency of replica plating onto the nylon cloth is affected by the colony size and the time of cloth-to-plate contact. Maximum replica plating efficiency (80-90%) occurs using 5- to 6-day-old colonies and a contact time of 4 h. The method easily allows 10(4) colonies to be examined for mutant phenotypes. The effectiveness of the technique is demonstrated by isolation of a clone deficient in activity of glucose-6-phosphate dehydrogenase.
